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Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth

  • Authors:
    • Ruijie Zhang
    • Dana M. Roque
    • Jocelyn Reader
    • Jiayuh Lin
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430000, P.R. China, Division of Gynecologic Oncology, Marlene and Stewart Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 50
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    Published online on: March 21, 2022
       https://doi.org/10.3892/ijo.2022.5340
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Abstract

Ovarian cancer is the most lethal gynecological cancer type in the United States. The success of current chemotherapies is limited by chemoresistance and side effects. Targeted therapy is a promising future direction for cancer therapy. In the present study, the efficacy of co‑targeting IL‑6 and IL‑8 in human ovarian cancer cells by bazedoxifene (Baze) + SCH527123 (SCH) treatment was examined. ELISA, cell viability, cell proliferation, cell migration, cell invasion, western blotting and peritoneal ovarian tumor mouse model analyses were performed to analyze the expression levels of IL‑6 and IL‑8, tumor growth, tumor migration and invasion, and the possible pathways of human ovarian cancer cell lines (SKOV3, CAOV3 and OVCAR3) and patient‑derived OV75 ovarian cancer cells. Each cell line was treated by monotherapy or combination therapy. The results demonstrated that IL‑6 and IL‑8 were secreted by human ovarian cancer cell lines. Compared with the DMSO control, the combination of IL‑6/glycoprotein 130 inhibitor Baze and IL‑8 inhibitor SCH synergistically inhibited cell viability in ovarian cancer cells. Baze + SCH also inhibited cell migration and invasion, suppressed ovarian tumor growth and inhibited STAT3 and AKT phosphorylation, as well as survivin expression. Therefore, co‑targeting the IL‑6 and IL‑8 signaling pathways may be an effective approach for ovarian cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang R, Roque DM, Reader J and Lin J: Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth. Int J Oncol 60: 50, 2022.
APA
Zhang, R., Roque, D.M., Reader, J., & Lin, J. (2022). Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth. International Journal of Oncology, 60, 50. https://doi.org/10.3892/ijo.2022.5340
MLA
Zhang, R., Roque, D. M., Reader, J., Lin, J."Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth". International Journal of Oncology 60.5 (2022): 50.
Chicago
Zhang, R., Roque, D. M., Reader, J., Lin, J."Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth". International Journal of Oncology 60, no. 5 (2022): 50. https://doi.org/10.3892/ijo.2022.5340
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang R, Roque DM, Reader J and Lin J: Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth. Int J Oncol 60: 50, 2022.
APA
Zhang, R., Roque, D.M., Reader, J., & Lin, J. (2022). Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth. International Journal of Oncology, 60, 50. https://doi.org/10.3892/ijo.2022.5340
MLA
Zhang, R., Roque, D. M., Reader, J., Lin, J."Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth". International Journal of Oncology 60.5 (2022): 50.
Chicago
Zhang, R., Roque, D. M., Reader, J., Lin, J."Combined inhibition of IL‑6 and IL‑8 pathways suppresses ovarian cancer cell viability and migration and tumor growth". International Journal of Oncology 60, no. 5 (2022): 50. https://doi.org/10.3892/ijo.2022.5340
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