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Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure

  • Authors:
    • Tobias R. Spuldaro
    • Vivian P. Wagner
    • Felipe Nör
    • Eduardo J. Gaio
    • Cristiane H. Squarize
    • Vinicius C. Carrard
    • Cassiano K. Rösing
    • Rogerio M. Castilho
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology, Federal University of Rio Grande do Sul, Porto Alegre, RS 90010‑150, Brazil, Department of Oral Pathology, Federal University of Rio Grande do Sul, Porto Alegre, RS 90010‑150, Brazil, Department of Periodontics and Oral Medicine, University of Michigan, Ann Arbor, MI 48109‑1078, USA
    Copyright: © Spuldaro et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 77
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    Published online on: May 5, 2022
       https://doi.org/10.3892/ijo.2022.5367
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Abstract

For decades, the link between poor oral hygiene and the increased prevalence of oral cancer has been suggested. Most recently, emerging evidence has suggested that chronic inflammatory diseases from the oral cavity (e.g., periodontal disease), to some extent, play a role in the development of oral squamous cell carcinoma (OSCC). The present study aimed to explore the direct impact of biofilm‑induced periodontitis in the carcinogenesis process using a tobacco surrogate animal model for oral cancer. A total of 42 Wistar rats were distributed into four experimental groups: Control group, periodontitis (Perio) group, 4‑nitroquinoline 1‑oxide (4‑NQO) group and 4NQO/Perio group. Periodontitis was stimulated by placing a ligature subgingivally, while oral carcinogenesis was induced by systemic administration of 4NQO in the drinking water for 20 weeks. It was observed that the Perio, 4NQO and 4NQO/Perio groups presented with significantly higher alveolar bone loss compared with that in the control group. Furthermore, all groups receiving 4NQO developed lesions on the dorsal surface of the tongue; however, the 4NQO/Perio group presented larger lesions compared with the 4NQO group. There was also a modest overall increase in the number of epithelial dysplasia and OSCC lesions in the 4NQO/Perio group. Notably, abnormal focal activation of cellular differentiation (cytokeratin 10‑positive cells) that extended near the basal cell layer of the mucosa was observed in rats receiving 4NQO alone, but was absent in rats receiving 4NQO and presenting with periodontal disease. Altogether, the presence of periodontitis combined with 4NQO administration augmented tumor size in the current rat model and tampered with the protective mechanisms of the cellular differentiation of epithelial cells.
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Copy and paste a formatted citation
Spandidos Publications style
Spuldaro TR, Wagner VP, Nör F, Gaio EJ, Squarize CH, Carrard VC, Rösing CK and Castilho RM: Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure. Int J Oncol 60: 77, 2022.
APA
Spuldaro, T.R., Wagner, V.P., Nör, F., Gaio, E.J., Squarize, C.H., Carrard, V.C. ... Castilho, R.M. (2022). Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure. International Journal of Oncology, 60, 77. https://doi.org/10.3892/ijo.2022.5367
MLA
Spuldaro, T. R., Wagner, V. P., Nör, F., Gaio, E. J., Squarize, C. H., Carrard, V. C., Rösing, C. K., Castilho, R. M."Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure". International Journal of Oncology 60.6 (2022): 77.
Chicago
Spuldaro, T. R., Wagner, V. P., Nör, F., Gaio, E. J., Squarize, C. H., Carrard, V. C., Rösing, C. K., Castilho, R. M."Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure". International Journal of Oncology 60, no. 6 (2022): 77. https://doi.org/10.3892/ijo.2022.5367
Copy and paste a formatted citation
x
Spandidos Publications style
Spuldaro TR, Wagner VP, Nör F, Gaio EJ, Squarize CH, Carrard VC, Rösing CK and Castilho RM: Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure. Int J Oncol 60: 77, 2022.
APA
Spuldaro, T.R., Wagner, V.P., Nör, F., Gaio, E.J., Squarize, C.H., Carrard, V.C. ... Castilho, R.M. (2022). Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure. International Journal of Oncology, 60, 77. https://doi.org/10.3892/ijo.2022.5367
MLA
Spuldaro, T. R., Wagner, V. P., Nör, F., Gaio, E. J., Squarize, C. H., Carrard, V. C., Rösing, C. K., Castilho, R. M."Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure". International Journal of Oncology 60.6 (2022): 77.
Chicago
Spuldaro, T. R., Wagner, V. P., Nör, F., Gaio, E. J., Squarize, C. H., Carrard, V. C., Rösing, C. K., Castilho, R. M."Periodontal disease affects oral cancer progression in a surrogate animal model for tobacco exposure". International Journal of Oncology 60, no. 6 (2022): 77. https://doi.org/10.3892/ijo.2022.5367
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