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FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma

  • Authors:
    • Yulin Yuan
    • Zimeng Liu
    • Bohan Li
    • Zheng Gong
    • Chiyuan Piao
    • Zhuonan Liu
    • Zhe Zhang
    • Xiao Dong
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Hospital of China Medical University, Shenyang, Liaoning 110002, P.R. China, Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, Liaoning 110002, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 40
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    Published online on: February 15, 2023
       https://doi.org/10.3892/ijo.2023.5488
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Abstract

Studies on clear cell renal cell carcinoma (ccRCC) are gaining momentum due to its high malignancy and potential to metastasize. F‑box protein 30 (FBXO30) is a member of the F‑box protein family; however, its role and mechanism in cancer remains to be fully elucidated. Western blotting, reverse transcription‑quantitative PCR and immunohistochemsitry were performed to detect the expression levels of FBXO30 in ccRCC tissues and adjacent normal tissues. Tumor biological function assays and animal experiments were conducted to clarify the inhibitory effect of FBXO30 on the progression and metastasis of ccRCC. Protein half‑life assay, MG132 inhibition assay, immunofluorescence assay and co‑immunoprecipitation assay were performed to explore the ubiquitination mechanism of FBXO30 and HIF‑1α. Zinc supplementation assay was used to verify the regulatory relationship between human ZRT, IRT‑like protein 1 (hZIP1), FBXO30 and HIF‑1α. The present study revealed that the expression levels of FBXO30 were lower in ccRCC tissues compared with those in normal adjacent tissues. In addition, FBXO30 inhibited the tumorigenesis and metastatic capacity of ccRCC cells in vivo and in vitro. FBXO30 mediated the ubiquitination and degradation of hypoxia‑inducible factor‑1α (HIF‑1α) in ccRCC cells under normoxia, thereby inhibiting the oncogenic effect of HIF‑1α. Notably, hZIP1 served as an upstream regulator of FBXO30, regulating the expression of FBXO30 and HIF‑1α by recruiting Zn2+. In conclusion, the present data suggested that FBXO30 is a novel E3 ubiquitination ligase that can function as a tumor suppressor in ccRCC, and the hZIP1/Zn2+/FBXO30/HIF‑1α axis may provide potential biomarkers or therapeutic targets for ccRCC.
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Copy and paste a formatted citation
Spandidos Publications style
Yuan Y, Liu Z, Li B, Gong Z, Piao C, Liu Z, Zhang Z and Dong X: FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma. Int J Oncol 62: 40, 2023.
APA
Yuan, Y., Liu, Z., Li, B., Gong, Z., Piao, C., Liu, Z. ... Dong, X. (2023). FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma. International Journal of Oncology, 62, 40. https://doi.org/10.3892/ijo.2023.5488
MLA
Yuan, Y., Liu, Z., Li, B., Gong, Z., Piao, C., Liu, Z., Zhang, Z., Dong, X."FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma". International Journal of Oncology 62.3 (2023): 40.
Chicago
Yuan, Y., Liu, Z., Li, B., Gong, Z., Piao, C., Liu, Z., Zhang, Z., Dong, X."FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma". International Journal of Oncology 62, no. 3 (2023): 40. https://doi.org/10.3892/ijo.2023.5488
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Y, Liu Z, Li B, Gong Z, Piao C, Liu Z, Zhang Z and Dong X: FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma. Int J Oncol 62: 40, 2023.
APA
Yuan, Y., Liu, Z., Li, B., Gong, Z., Piao, C., Liu, Z. ... Dong, X. (2023). FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma. International Journal of Oncology, 62, 40. https://doi.org/10.3892/ijo.2023.5488
MLA
Yuan, Y., Liu, Z., Li, B., Gong, Z., Piao, C., Liu, Z., Zhang, Z., Dong, X."FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma". International Journal of Oncology 62.3 (2023): 40.
Chicago
Yuan, Y., Liu, Z., Li, B., Gong, Z., Piao, C., Liu, Z., Zhang, Z., Dong, X."FBXO30 functions as a tumor suppressor and an E3 ubiquitin ligase for hZIP1‑mediated HIF‑1α degradation in renal cell carcinoma". International Journal of Oncology 62, no. 3 (2023): 40. https://doi.org/10.3892/ijo.2023.5488
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