Downregulation of miR‑7 and miR‑153 is involved in Helicobacter pylori CagA induced gastric carcinogenesis and progression
Affiliations: Department of Central Lab, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, Shandong 264200, P.R. China, Department of Gastroenterology, Weihai Municipal Hospital, Weihai, Shandong 264200, P.R. China, Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, SAR 999077, P.R. China, Marie College, Shandong University, Weihai, Shandong 264209, P.R. China
- Published online on: May 24, 2023 https://doi.org/10.3892/ijo.2023.5527
- Article Number: 79
Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Helicobacter pylori (H. pylori) infection plays a pivotal role in the development of gastric cancer (GC). However, the association between aberrant microRNAs (miRNAs/miRs) expression and H. pylori‑induced GC remains poorly understood. The present study reported that repeated infection of H. pylori caused the oncogenicity of GES‑1 cells in BALB/c Nude mice. miRNA sequencing revealed that both miR‑7 and miR‑153 were significantly decreased in the cytotoxin‑associated gene A (CagA) positive GC tissues and this was further confirmed in a chronic infection model of GES‑1/HP cells. Further biological function experiments and in vivo experiments validated that miR‑7 and miR‑153 can promote apoptosis and autophagy, inhibit proliferation and inflammatory response in GES‑1/HP cells. All the associations between miR‑7/miR‑153 and their potential targets were revealed via bioinformatics prediction and dual‑luciferase reporter assay. Particularly, downregulation of both miR‑7 and miR‑153 obtained an improved sensitivity and specificity in diagnosing H. pylori (CagA+)‑induced GC. The present study identified that the combination of miR‑7 and miR‑153 may be regarded as novel therapeutic targets in H. pylori CagA (+)‑associated GC.