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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2003 Volume 23 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2003 Volume 23 Issue 3

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Article

N-glycosylation is required for the surface localization of MUC17 mucin

  • Authors:
    • Jenny J.L. Ho
    • Reena S. Jaituni
    • Suzanne C. Crawley
    • Stacey C. Yang
    • James R. Gum
    • Young S. Kim

  • View Affiliations / Copyright

    Affiliations: Gastrointestinal Research Laboratory (151 M2), Veterans Affairs Medical Center, San Francisco, CA 94121, USA. scinthesis@yahoo.com
  • Pages: 585-592
    |
    Published online on: September 1, 2003
       https://doi.org/10.3892/ijo.23.3.585
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Abstract

The nucleic acid sequence of the human gene, MUC17, indicates that this mucin contains an SEA domain, a transmembrane domain, and putative N-glycosylation sites in the carboxyl terminus. Mucins that possess an SEA domain are usually proteolytically cleaved within that domain to yield two subunits, the smaller of which is associated with the surface membrane. Homogenates of ASPC-1 pancreatic cancer cells showed three main bands of immunoreactivity with α-SEA (a polyclonal antibody directed against a site downstream of the postulated cleavage site) after SDS-PAGE and Western blotting (38, 45, and 49 kDa). Experiments utilizing N-glycan specific hydrolases showed that the 38 kDa band contained high mannose glycans whereas the 45 and 49 kDa bands contained complex-type glycans. Only two smaller α-SEA reactive bands (30 and 32 kDa) were present after cells had been treated with the N-glycosylation inhibitor tunicamycin. Surface biotinylation studies showed that only the forms possessing complex-type N-glycans were localized to the cell surface. Both tunicamycin and brefeldin A, an inhibitor of protein transport, reduced surface localization. In summary, our results indicate that the surface localization of the smaller subunit of MUC17 is dependent on its N-glycosylation status.

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Copy and paste a formatted citation
Spandidos Publications style
Ho JJ, Jaituni RS, Crawley SC, Yang SC, Gum JR and Kim YS: N-glycosylation is required for the surface localization of MUC17 mucin. Int J Oncol 23: 585-592, 2003.
APA
Ho, J.J., Jaituni, R.S., Crawley, S.C., Yang, S.C., Gum, J.R., & Kim, Y.S. (2003). N-glycosylation is required for the surface localization of MUC17 mucin. International Journal of Oncology, 23, 585-592. https://doi.org/10.3892/ijo.23.3.585
MLA
Ho, J. J., Jaituni, R. S., Crawley, S. C., Yang, S. C., Gum, J. R., Kim, Y. S."N-glycosylation is required for the surface localization of MUC17 mucin". International Journal of Oncology 23.3 (2003): 585-592.
Chicago
Ho, J. J., Jaituni, R. S., Crawley, S. C., Yang, S. C., Gum, J. R., Kim, Y. S."N-glycosylation is required for the surface localization of MUC17 mucin". International Journal of Oncology 23, no. 3 (2003): 585-592. https://doi.org/10.3892/ijo.23.3.585
Copy and paste a formatted citation
x
Spandidos Publications style
Ho JJ, Jaituni RS, Crawley SC, Yang SC, Gum JR and Kim YS: N-glycosylation is required for the surface localization of MUC17 mucin. Int J Oncol 23: 585-592, 2003.
APA
Ho, J.J., Jaituni, R.S., Crawley, S.C., Yang, S.C., Gum, J.R., & Kim, Y.S. (2003). N-glycosylation is required for the surface localization of MUC17 mucin. International Journal of Oncology, 23, 585-592. https://doi.org/10.3892/ijo.23.3.585
MLA
Ho, J. J., Jaituni, R. S., Crawley, S. C., Yang, S. C., Gum, J. R., Kim, Y. S."N-glycosylation is required for the surface localization of MUC17 mucin". International Journal of Oncology 23.3 (2003): 585-592.
Chicago
Ho, J. J., Jaituni, R. S., Crawley, S. C., Yang, S. C., Gum, J. R., Kim, Y. S."N-glycosylation is required for the surface localization of MUC17 mucin". International Journal of Oncology 23, no. 3 (2003): 585-592. https://doi.org/10.3892/ijo.23.3.585
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