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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2003 Volume 23 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2003 Volume 23 Issue 3

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Article

Detection of circulating cancer cells in human oral squamous cell carcinoma

  • Authors:
    • Hirofumi Zen
    • Koh-Ichi Nakashiro
    • Satoru Shintani
    • Tomoki Sumida
    • Takayoshi Aramoto
    • Hiroyuki Hamakawa

  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Ehime University School of Medicine, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan
  • Pages: 605-610
    |
    Published online on: September 1, 2003
       https://doi.org/10.3892/ijo.23.3.605
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Abstract

Oral squamous cell carcinoma (OSCC) cells can invade adjacent tissues and the vascular system at an early stage of tumor progression. In the present study, we have attempted to detect circulating cancer cells. We used human OSCC cells expressing green fluorescent protein (GFP) in an orthotopic nude mouse model and were able to detect GFP-expressing cells using a fluorescence activated cell sorter and fluorescence microscopy. We also detected the expression of GFP, squamous cell carcinoma antigen (SCCA), or epidermal growth factor receptor (EGFR) mRNA in the blood of tumor-bearing mice by conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR (TaqMan RT-PCR). TaqMan RT-PCR was the more sensitive method to detect the circulating cancer cells. Furthermore, we examined the expression of SCCA and EGFR mRNA in the peripheral blood of patients with OSCC by conventional RT-PCR and TaqMan RT-PCR. We detected SCCA and EGFR mRNA in few cases by conventional RT-PCR, whereas TaqMan RT-PCR showed their expression in about 50% of cases. However, there was no correlation between detection of circulating viable cancer cells and metastasis to lymph nodes and distant organs. These results suggest that TaqMan RT-PCR is a very useful method and both SCCA and EGFR mRNA may be available as a marker for detection of circulating cancer cells.

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Copy and paste a formatted citation
Spandidos Publications style
Zen H, Nakashiro K, Shintani S, Sumida T, Aramoto T and Hamakawa H: Detection of circulating cancer cells in human oral squamous cell carcinoma. Int J Oncol 23: 605-610, 2003.
APA
Zen, H., Nakashiro, K., Shintani, S., Sumida, T., Aramoto, T., & Hamakawa, H. (2003). Detection of circulating cancer cells in human oral squamous cell carcinoma. International Journal of Oncology, 23, 605-610. https://doi.org/10.3892/ijo.23.3.605
MLA
Zen, H., Nakashiro, K., Shintani, S., Sumida, T., Aramoto, T., Hamakawa, H."Detection of circulating cancer cells in human oral squamous cell carcinoma". International Journal of Oncology 23.3 (2003): 605-610.
Chicago
Zen, H., Nakashiro, K., Shintani, S., Sumida, T., Aramoto, T., Hamakawa, H."Detection of circulating cancer cells in human oral squamous cell carcinoma". International Journal of Oncology 23, no. 3 (2003): 605-610. https://doi.org/10.3892/ijo.23.3.605
Copy and paste a formatted citation
x
Spandidos Publications style
Zen H, Nakashiro K, Shintani S, Sumida T, Aramoto T and Hamakawa H: Detection of circulating cancer cells in human oral squamous cell carcinoma. Int J Oncol 23: 605-610, 2003.
APA
Zen, H., Nakashiro, K., Shintani, S., Sumida, T., Aramoto, T., & Hamakawa, H. (2003). Detection of circulating cancer cells in human oral squamous cell carcinoma. International Journal of Oncology, 23, 605-610. https://doi.org/10.3892/ijo.23.3.605
MLA
Zen, H., Nakashiro, K., Shintani, S., Sumida, T., Aramoto, T., Hamakawa, H."Detection of circulating cancer cells in human oral squamous cell carcinoma". International Journal of Oncology 23.3 (2003): 605-610.
Chicago
Zen, H., Nakashiro, K., Shintani, S., Sumida, T., Aramoto, T., Hamakawa, H."Detection of circulating cancer cells in human oral squamous cell carcinoma". International Journal of Oncology 23, no. 3 (2003): 605-610. https://doi.org/10.3892/ijo.23.3.605
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