Expression of HER family receptors and effects of anti-HER2-antibody on human myeloma cell lines

  • Authors:
    • Takemi Otsuki
    • Junichi Kurebayashi
    • Sumiko Ohkubo
    • Masako Uno
    • Tomohiro Fujii
    • Haruko Sakaguchi
    • Tamayo Hatayama
    • Akiko Takata
    • Takayuki Tsujioka
    • Takashi Sugihara
    • Fuminori Hyodoh
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.1135
  • Pages: 1135-1141
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Abstract

We have recently studied expression of estrogen receptors and the growth inhibitory effects of antiestrogens on human myeloma cells. In myeloma chemotherapy, Antiestrogens in combination with other chemotherapeutic agents, may have applications in which melphalan/predonisolone still remains the standard treatment. In this study, we examined expression of HER family molecules in myeloma cells to clarify the possible usage of anti-HER2-monoclonal antibody in the treatment of myeloma. Although the mRNA levels of HER family genes analyzed by RT-PCR were significantly lower in myeloma cells than breast cancer cells, some cell lines expressed a certain amount of HER2 and HER4 proteins. In addition, an anti-HER2 monoclonal antibody, rhumAbHER2, caused significant growth inhibition in six out of eight myeloma cell lines studied and these inhibitory effects were similar to those in the breast cancer cells studied previously. The rhumAbHER2 induced up-regulation of p21 family CDK-Is (cyclin dependent kinase inhibitors) and down-regulation of VEGF genes. Moreover, combination treatment with antiestrogen had an additive growth inhibitory effect. Such analyses may provide for use of rhumAbHER2 in myeloma treatment for the future.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Otsuki T, Kurebayashi J, Ohkubo S, Uno M, Fujii T, Sakaguchi H, Hatayama T, Takata A, Tsujioka T, Sugihara T, Sugihara T, et al: Expression of HER family receptors and effects of anti-HER2-antibody on human myeloma cell lines. Int J Oncol 23: 1135-1141, 2003
APA
Otsuki, T., Kurebayashi, J., Ohkubo, S., Uno, M., Fujii, T., Sakaguchi, H. ... Hyodoh, F. (2003). Expression of HER family receptors and effects of anti-HER2-antibody on human myeloma cell lines. International Journal of Oncology, 23, 1135-1141. https://doi.org/10.3892/ijo.23.4.1135
MLA
Otsuki, T., Kurebayashi, J., Ohkubo, S., Uno, M., Fujii, T., Sakaguchi, H., Hatayama, T., Takata, A., Tsujioka, T., Sugihara, T., Hyodoh, F."Expression of HER family receptors and effects of anti-HER2-antibody on human myeloma cell lines". International Journal of Oncology 23.4 (2003): 1135-1141.
Chicago
Otsuki, T., Kurebayashi, J., Ohkubo, S., Uno, M., Fujii, T., Sakaguchi, H., Hatayama, T., Takata, A., Tsujioka, T., Sugihara, T., Hyodoh, F."Expression of HER family receptors and effects of anti-HER2-antibody on human myeloma cell lines". International Journal of Oncology 23, no. 4 (2003): 1135-1141. https://doi.org/10.3892/ijo.23.4.1135