Tetraarsenic oxide induces apoptosis in U937 leukemic cells through a reactive oxygen species-dependent pathway

  • Authors:
    • In-Chul Park
    • Myung-Jin Park
    • Sang-Hyeok Woo
    • Hyung-Chahn Lee
    • Sungkwan An
    • Ho-Shin Gwak
    • Seung-Hoon Lee
    • Seok-Il Hong
    • Ill-Ju Bae
    • Kang Moon Seo
    • Chang Hun Rhee
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.943
  • Pages: 943-948
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Abstract

In the present study, we investigated the effect of tetraarsenic oxide (As4O6, 2,4,6,8,9,10-Hexaoxa-1,3,5,7-tetraarsatricyclo[3.3.1.13,7]decane) upon induction of apoptosis in arsenic trioxide (diarsenic oxide, As2O3) resistant U937 leukemic cells. As4O6 induced apoptosis in U937 leukemic cells at much lower concentrations than As2O3 via an early increase of cellular reactive oxygen species (ROS), and a decrease in cellular mitochondrial membrane potential, followed by cytochrome c release and caspase-3 activation. As4O6 generated ROS and induced caspase-3 activation more potently than As2O3 in U937 cells. Incubation of the cells with N-acetyl-L-cysteine and catalase resulted in significant suppression of As4O6-induced apoptotic cell death. These results show that the generation of ROS leads to the consequences associated with apoptosis induced by As4O6. In conclusion, As4O6 might be a new arsenic compound which may induce apoptosis in U937 leukemic cells by activating unique apoptotic signaling mediated by ROS more potently than As2O3, and deserves further evaluation.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park I, Park M, Woo S, Lee H, An S, Gwak H, Lee S, Hong S, Bae I, Seo KM, Seo KM, et al: Tetraarsenic oxide induces apoptosis in U937 leukemic cells through a reactive oxygen species-dependent pathway. Int J Oncol 23: 943-948, 2003
APA
Park, I., Park, M., Woo, S., Lee, H., An, S., Gwak, H. ... Rhee, C.H. (2003). Tetraarsenic oxide induces apoptosis in U937 leukemic cells through a reactive oxygen species-dependent pathway. International Journal of Oncology, 23, 943-948. https://doi.org/10.3892/ijo.23.4.943
MLA
Park, I., Park, M., Woo, S., Lee, H., An, S., Gwak, H., Lee, S., Hong, S., Bae, I., Seo, K. M., Rhee, C. H."Tetraarsenic oxide induces apoptosis in U937 leukemic cells through a reactive oxygen species-dependent pathway". International Journal of Oncology 23.4 (2003): 943-948.
Chicago
Park, I., Park, M., Woo, S., Lee, H., An, S., Gwak, H., Lee, S., Hong, S., Bae, I., Seo, K. M., Rhee, C. H."Tetraarsenic oxide induces apoptosis in U937 leukemic cells through a reactive oxygen species-dependent pathway". International Journal of Oncology 23, no. 4 (2003): 943-948. https://doi.org/10.3892/ijo.23.4.943