Augmentation of Fas ligand-induced apoptosis by MUC1 mucin

  • Authors:
    • Rupesh Chaturvedi
    • Rakesh K. Srivastava
    • Akinori Hisatsune
    • Sharmila Shankar
    • Erik P. Lillehoj
    • K. Chul Kim
  • View Affiliations

  • Published online on: May 1, 2005     https://doi.org/10.3892/ijo.26.5.1169
  • Pages: 1169-1176
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Abstract

Apoptosis is a physiological mechanism responsible for a wide range of cellular processes during growth, development, carcinogenesis, and inflammation. In this study, we determined whether MUC1 affects Fas ligand (FasL)-induced apoptosis using MUC1 expressing (MUC1+) and MUC1− cells. Following treatment with 50 nM FasL, apoptosis, caspase-8 activity, and cell surface Fas receptor were measured by cytosolic nucleosome ELISA, colorimetric enzyme assay, and immunofluorescence analysis, respectively. Our results showed that (i) treatment with FasL increased caspase-8 activity (maximum at 4 h) and apoptosis (maximum at 8 h) in both MUC1+ and MUC1− cells, (ii) FasL-induced caspase-8 activity and apoptosis were significantly greater in MUC1+ cells compared with MUC1− cells, (iii) FasL treatment increased cell surface expression of Fas receptor in MUC1+ cells to a greater extent compared with MUC1− cells, (iv) increased cell surface expression of Fas in MUC1+ cells was not blocked by an inhibitor of protein synthesis (cycloheximide), but was completely abrogated by brefeldin A, an inhibitor of post-translational protein trafficking to the cell surface, and (v) brefeldin A inhibited the increased sensitivity of MUC1+ cells to FasL-induced apoptosis. We conclude that MUC1+ cells were more sensitive to FasL-induced apoptosis compared with MUC1− cells due to up-regulation of Fas receptor on the cell surface by a mechanism involving increased intracellular trafficking.

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May 2005
Volume 26 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Chaturvedi R, Srivastava RK, Hisatsune A, Shankar S, Lillehoj EP and Kim KC: Augmentation of Fas ligand-induced apoptosis by MUC1 mucin. Int J Oncol 26: 1169-1176, 2005
APA
Chaturvedi, R., Srivastava, R.K., Hisatsune, A., Shankar, S., Lillehoj, E.P., & Kim, K.C. (2005). Augmentation of Fas ligand-induced apoptosis by MUC1 mucin. International Journal of Oncology, 26, 1169-1176. https://doi.org/10.3892/ijo.26.5.1169
MLA
Chaturvedi, R., Srivastava, R. K., Hisatsune, A., Shankar, S., Lillehoj, E. P., Kim, K. C."Augmentation of Fas ligand-induced apoptosis by MUC1 mucin". International Journal of Oncology 26.5 (2005): 1169-1176.
Chicago
Chaturvedi, R., Srivastava, R. K., Hisatsune, A., Shankar, S., Lillehoj, E. P., Kim, K. C."Augmentation of Fas ligand-induced apoptosis by MUC1 mucin". International Journal of Oncology 26, no. 5 (2005): 1169-1176. https://doi.org/10.3892/ijo.26.5.1169