Detection of cryptic chromosomal aberrations in the in vitro non-proliferating cells of acute myeloid leukemia

  • Authors:
    • Constanze Karst
    • Anita Heller
    • Uwe Claussen
    • Erich Gebhart
    • Thomas Liehr
  • View Affiliations

  • Published online on: August 1, 2005     https://doi.org/10.3892/ijo.27.2.355
  • Pages: 355-359
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Abstract

A total of 22 acute myeloid leukemia (AML) cases were analyzed by cell-specific comparative genomic hybridization (micro-CGH). Conventional banding analysis identified a monosomy 7 in six (group I), a trisomy 8 in eight (group II) and a normal karyotype in eight cases (group III). A total of 32 additional chromosomal imbalances was detected and confirmed in two independent micro-CGH experiments. However, only in 9 of the 22 cases (group I: 4 cases; group II: 1 case; group III: 4 cases) the existence of 11 of the 32 (34.5%) detected copy number alterations could be confirmed by other fluorescence in situ hybridization (FISH) approaches. These results lead to two conclusions: i) in the in vitro non-proliferating population of AML tumor cells one can detect cryptic chromosomal aberrations, which might constitute tumor markers of diagnostic and prognostic value; ii) The results of CGH need to be checked by other approaches.

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August 2005
Volume 27 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Karst C, Heller A, Claussen U, Gebhart E and Liehr T: Detection of cryptic chromosomal aberrations in the in vitro non-proliferating cells of acute myeloid leukemia. Int J Oncol 27: 355-359, 2005
APA
Karst, C., Heller, A., Claussen, U., Gebhart, E., & Liehr, T. (2005). Detection of cryptic chromosomal aberrations in the in vitro non-proliferating cells of acute myeloid leukemia. International Journal of Oncology, 27, 355-359. https://doi.org/10.3892/ijo.27.2.355
MLA
Karst, C., Heller, A., Claussen, U., Gebhart, E., Liehr, T."Detection of cryptic chromosomal aberrations in the in vitro non-proliferating cells of acute myeloid leukemia". International Journal of Oncology 27.2 (2005): 355-359.
Chicago
Karst, C., Heller, A., Claussen, U., Gebhart, E., Liehr, T."Detection of cryptic chromosomal aberrations in the in vitro non-proliferating cells of acute myeloid leukemia". International Journal of Oncology 27, no. 2 (2005): 355-359. https://doi.org/10.3892/ijo.27.2.355