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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2005 Volume 27 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2005 Volume 27 Issue 4

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Article

The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells

  • Authors:
    • Jakub Neradil
    • Renata Veselská
    • Augustin Svoboda

  • View Affiliations / Copyright

    Affiliations: Cell Culture Laboratory, Department of Biology, Medical Faculty, Masaryk University in Brno, Brno, Czech Republic
  • Pages: 1013-1021
    |
    Published online on: October 1, 2005
       https://doi.org/10.3892/ijo.27.4.1013
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Abstract

The P19 mouse embryonal carcinoma cell line was used as a model for a study of apoptosis accompanying differentiation induced by all-trans retinoic acid (ATRA). Apoptosis was detected both on the basis of morphological features (nuclear fragmentation, blebbing of plasma membrane, and formation of apoptotic bodies), and by using DNA electrophoresis and flow-cytometric measurement of DNA content. Actin cytoskeleton was studied both on morphological and submicroscopic levels. ATRA-treated cells manifested apoptosis-specific changes in the distribution of actin foremost in association with their entry into executive phase of apoptosis, when F-actin cables participated in cell disintegration into apoptotic bodies. Using immunogold labeling, actin was also identified in centers of fragmenting apoptotic nuclei, in the disintegration of which it is likely involved as well. At the same time, a cleavage of actin by active caspase-3 was proved, resulting in the emergence of 32 kDa fragment, termed fractin. Measurement of F-actin and fractin content using flow cytometry showed an unequivocal decrease of F-actin and synchronous increase of fractin in the apoptotic population as compared to non-treated cells. Therefore, our results proved both actin proteolysis and active involvement of specific actin structures in the final cell disintegration during apoptosis in the P19 cells.

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Copy and paste a formatted citation
Spandidos Publications style
Neradil J, Veselská R and Svoboda A: The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells. Int J Oncol 27: 1013-1021, 2005.
APA
Neradil, J., Veselská, R., & Svoboda, A. (2005). The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells. International Journal of Oncology, 27, 1013-1021. https://doi.org/10.3892/ijo.27.4.1013
MLA
Neradil, J., Veselská, R., Svoboda, A."The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells". International Journal of Oncology 27.4 (2005): 1013-1021.
Chicago
Neradil, J., Veselská, R., Svoboda, A."The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells". International Journal of Oncology 27, no. 4 (2005): 1013-1021. https://doi.org/10.3892/ijo.27.4.1013
Copy and paste a formatted citation
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Spandidos Publications style
Neradil J, Veselská R and Svoboda A: The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells. Int J Oncol 27: 1013-1021, 2005.
APA
Neradil, J., Veselská, R., & Svoboda, A. (2005). The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells. International Journal of Oncology, 27, 1013-1021. https://doi.org/10.3892/ijo.27.4.1013
MLA
Neradil, J., Veselská, R., Svoboda, A."The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells". International Journal of Oncology 27.4 (2005): 1013-1021.
Chicago
Neradil, J., Veselská, R., Svoboda, A."The role of actin in the apoptotic cell death of P19 embryonal carcinoma cells". International Journal of Oncology 27, no. 4 (2005): 1013-1021. https://doi.org/10.3892/ijo.27.4.1013
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