GTI-2501, an antisense agent targeting R1, the large subunit of human ribonucleotide reductase, shows potent anti-tumor activity against a variety of tumors

  • Authors:
    • Yoon Lee
    • Aikaterini Vassilakos
    • Ningping Feng
    • Hongnan Jin
    • Ming Wang
    • Keyong Xiong
    • Jim Wright
    • Aiping Young
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/ijo.28.2.469
  • Pages: 469-478
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Abstract

GTI-2501 is a 20-mer oligonucleotide that is complementary to a coding region in the mRNA of R1, the large subunit of ribonucleotide reductase (RNR). In vitro studies, have demonstrated that GTI-2501 decreases mRNA and protein levels of R1 in a sequence-specific and dose-dependent manner. Furthermore, GTI-2501 inhibits the growth of human lung, liver, ovary, brain, melanoma, breast and pancreatic tumor cells in colony forming assays. In vivo studies have shown that GTI-2501 significantly inhibits growth of human colon, pancreas, lung, breast, renal, ovarian, melanoma, brain glioblastoma-astrocytoma, and prostatic tumors in CD-1 nude, Balb/c nude and/or SCID mice. GTI-2501 treatment caused total regression of human breast and renal tumor xenografts in mice. These effects are not observed with a scrambled control oligonucleotide containing the same base content but not complementary to R1. GTI-2501 specifically inhibits metastasis of human melanoma cells to the lungs in CD-1 athymic nude mice and prolongs the survival of mice bearing human lymphoma. Taken together these results suggest that an antisense mechanism of action is responsible for growth inhibition in vitro and in vivo and that GTI-2501 can act as a selective and specific anti-tumor agent.

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February 2006
Volume 28 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lee Y, Vassilakos A, Feng N, Jin H, Wang M, Xiong K, Wright J and Young A: GTI-2501, an antisense agent targeting R1, the large subunit of human ribonucleotide reductase, shows potent anti-tumor activity against a variety of tumors. Int J Oncol 28: 469-478, 2006
APA
Lee, Y., Vassilakos, A., Feng, N., Jin, H., Wang, M., Xiong, K. ... Young, A. (2006). GTI-2501, an antisense agent targeting R1, the large subunit of human ribonucleotide reductase, shows potent anti-tumor activity against a variety of tumors. International Journal of Oncology, 28, 469-478. https://doi.org/10.3892/ijo.28.2.469
MLA
Lee, Y., Vassilakos, A., Feng, N., Jin, H., Wang, M., Xiong, K., Wright, J., Young, A."GTI-2501, an antisense agent targeting R1, the large subunit of human ribonucleotide reductase, shows potent anti-tumor activity against a variety of tumors". International Journal of Oncology 28.2 (2006): 469-478.
Chicago
Lee, Y., Vassilakos, A., Feng, N., Jin, H., Wang, M., Xiong, K., Wright, J., Young, A."GTI-2501, an antisense agent targeting R1, the large subunit of human ribonucleotide reductase, shows potent anti-tumor activity against a variety of tumors". International Journal of Oncology 28, no. 2 (2006): 469-478. https://doi.org/10.3892/ijo.28.2.469