Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ

  • Authors:
    • F. H. Schmitz-Winnenthal
    • L. V. Galindo Escobedo
    • P. Beckhove
    • V. Schirrmacher
    • M. Bucur
    • Y. Ziouta
    • C. Volk
    • B. Schmied
    • M. Koch
    • D. Antolovic
    • J. Weitz
    • M. W. Büchler
    • K. Z'graggen
  • View Affiliations

  • Published online on: June 1, 2006     https://doi.org/10.3892/ijo.28.6.1419
  • Pages: 1419-1428
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Abstract

Pancreatic carcinoma is a very aggressive disease and little is known about its immunobiology. We here describe the presence in pancreatic cancer patients of spontaneously induced functional CD4 and CD8 memory/effector T cells reactive to autologous tumor cells or to the pancreatic cancer associated antigen, MUC-1. Such specific cells were present in the bone marrow or peripheral blood of most of the 23 tested patients. Low dose stimulation of primary cultures of pancreatic cancer cells with 500 IU/ml IFN-γ for 72 h enhanced HLA-I expression and induced the de novo expression of HLA-II molecules. This led to a much better immune recognition by autologous HLA-I restricted and purified CD8 T cells and allowed tumor cell recognition by HLA-II restricted purified CD4 T-helper cells. Thus, interferon-γ appears to be a useful adjuvant cytokine to enhance the immunogenicity of a patients' tumor cells and their recognition by tumor reactive immune cells.

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June 2006
Volume 28 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Schmitz-Winnenthal FH, Galindo Escobedo LV, Beckhove P, Schirrmacher V, Bucur M, Ziouta Y, Volk C, Schmied B, Koch M, Antolovic D, Antolovic D, et al: Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ. Int J Oncol 28: 1419-1428, 2006
APA
Schmitz-Winnenthal, F.H., Galindo Escobedo, L.V., Beckhove, P., Schirrmacher, V., Bucur, M., Ziouta, Y. ... Z'graggen, K. (2006). Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ. International Journal of Oncology, 28, 1419-1428. https://doi.org/10.3892/ijo.28.6.1419
MLA
Schmitz-Winnenthal, F. H., Galindo Escobedo, L. V., Beckhove, P., Schirrmacher, V., Bucur, M., Ziouta, Y., Volk, C., Schmied, B., Koch, M., Antolovic, D., Weitz, J., Büchler, M. W., Z'graggen, K."Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ". International Journal of Oncology 28.6 (2006): 1419-1428.
Chicago
Schmitz-Winnenthal, F. H., Galindo Escobedo, L. V., Beckhove, P., Schirrmacher, V., Bucur, M., Ziouta, Y., Volk, C., Schmied, B., Koch, M., Antolovic, D., Weitz, J., Büchler, M. W., Z'graggen, K."Specific immune recognition of pancreatic carcinoma by patient-derived CD4 and CD8 T cells and its improvement by interferon-γ". International Journal of Oncology 28, no. 6 (2006): 1419-1428. https://doi.org/10.3892/ijo.28.6.1419