Targeting of cholecystokinin B/gastrin receptor in colonic, pancreatic and hepatocellular carcinoma cell lines

  • Authors:
    • Kay Savage
    • H. Anne Waller
    • Mark Stubbs
    • Korsa Khan
    • Susan A. Watson
    • Philip A. Clarke
    • Steve Grimes
    • Dov Michaeli
    • Amar P. Dhillon
    • Martyn E. Caplin
  • View Affiliations

  • Published online on: December 1, 2006     https://doi.org/10.3892/ijo.29.6.1429
  • Pages: 1429-1435
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Gastrin is a growth factor for both gastrointestinal and non-gastrointestinal tumours. Endocytosis of gastrin has been demonstrated in tumour cell lines expressing cholecystokinin-B/gastrin receptor (CCK-BR); this has raised the possibility of receptor targeted therapy. The aim of this study was to examine endocytosis of gastrin and CCK-BR in tumour cell lines. A small gastrin analogue, RG-G7, and the anti-CCK-BR antibody, anti-GRE1, were fluorescently labelled and uptake by cancer cell lines including AR42J, HepG2, and C170HM2 as well as transfected NIH3T3 fibroblast cells was assessed using standard and confocal fluorescence microscopy. CCK-BR expression of cell lines was assayed by reverse transcription-polymerase chain reaction and Western blotting. Apoptosis was detected using a fluorescent TUNEL method. RG-G7 and anti-GRE1 antibody were specifically taken up by all cell lines expressing CCK-BR. In addition to cytoplasmic uptake with RG-G7 and anti-GRE1 the latter also showed specific uptake into the nucleus. A coincidence of anti-GRE1 and apoptosis was seen. Targeting CCK-BR by peptide or antibody may offer therapeutic opportunities for some cancers.

Related Articles

Journal Cover

December 2006
Volume 29 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Savage K, Waller HA, Stubbs M, Khan K, Watson SA, Clarke PA, Grimes S, Michaeli D, Dhillon AP, Caplin ME, Caplin ME, et al: Targeting of cholecystokinin B/gastrin receptor in colonic, pancreatic and hepatocellular carcinoma cell lines. Int J Oncol 29: 1429-1435, 2006.
APA
Savage, K., Waller, H.A., Stubbs, M., Khan, K., Watson, S.A., Clarke, P.A. ... Caplin, M.E. (2006). Targeting of cholecystokinin B/gastrin receptor in colonic, pancreatic and hepatocellular carcinoma cell lines. International Journal of Oncology, 29, 1429-1435. https://doi.org/10.3892/ijo.29.6.1429
MLA
Savage, K., Waller, H. A., Stubbs, M., Khan, K., Watson, S. A., Clarke, P. A., Grimes, S., Michaeli, D., Dhillon, A. P., Caplin, M. E."Targeting of cholecystokinin B/gastrin receptor in colonic, pancreatic and hepatocellular carcinoma cell lines". International Journal of Oncology 29.6 (2006): 1429-1435.
Chicago
Savage, K., Waller, H. A., Stubbs, M., Khan, K., Watson, S. A., Clarke, P. A., Grimes, S., Michaeli, D., Dhillon, A. P., Caplin, M. E."Targeting of cholecystokinin B/gastrin receptor in colonic, pancreatic and hepatocellular carcinoma cell lines". International Journal of Oncology 29, no. 6 (2006): 1429-1435. https://doi.org/10.3892/ijo.29.6.1429