Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile

  • Authors:
    • Ryouichi Tsunedomi
    • Norio Iizuka
    • Hisafumi Yamada-Okabe
    • Takao Tamesa
    • Toshimasa Okada
    • Kazuhiko Sakamoto
    • Motonari Takashima
    • Takeshi Hamaguchi
    • Takanobu Miyamoto
    • Shunji Uchimura
    • Yoshihiko Hamamoto
    • Mamoru Yamada
    • Masaaki Oka
  • View Affiliations

  • Published online on: December 1, 2006     https://doi.org/10.3892/ijo.29.6.1445
  • Pages: 1445-1451
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Abstract

Portal vein invasion (PVI) is a hallmark of metastatic potential of hepatocellular carcinoma (HCC) and is frequently found at a stage of moderately differentiated HCC. To identify genes involved in PVI of HCC associated with hepatitis C virus (HCV), we performed a comprehensive analysis of 12,600 genes in 35 moderately differentiated HCV-related HCCs by DNA microarray. Our supervised learning method identified 35 genes involved in PVI. Among the 35 identified genes, we focused on the inhibitor of DNA binding 2 (ID2), because it encodes a liver-rich dominant-negative helix-loop-helix protein. The microarray results for ID2 were reproduced by quantitative real-time reverse transcription (QRT)-PCR and Western blot analyses. In an independent set of HCV-related HCCs (n=28) and HCV-unrelated HCCs (n=14), our QRT-PCR showed that decrease in ID2 mRNA levels were associated with PVI in HCV-related HCC but not HCV-unrelated HCC. In conclusion, our results strongly suggest that ID2 plays an important role in PVI process of HCV-related HCC.

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December 2006
Volume 29 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tsunedomi R, Iizuka N, Yamada-Okabe H, Tamesa T, Okada T, Sakamoto K, Takashima M, Hamaguchi T, Miyamoto T, Uchimura S, Uchimura S, et al: Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile. Int J Oncol 29: 1445-1451, 2006
APA
Tsunedomi, R., Iizuka, N., Yamada-Okabe, H., Tamesa, T., Okada, T., Sakamoto, K. ... Oka, M. (2006). Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile. International Journal of Oncology, 29, 1445-1451. https://doi.org/10.3892/ijo.29.6.1445
MLA
Tsunedomi, R., Iizuka, N., Yamada-Okabe, H., Tamesa, T., Okada, T., Sakamoto, K., Takashima, M., Hamaguchi, T., Miyamoto, T., Uchimura, S., Hamamoto, Y., Yamada, M., Oka, M."Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile". International Journal of Oncology 29.6 (2006): 1445-1451.
Chicago
Tsunedomi, R., Iizuka, N., Yamada-Okabe, H., Tamesa, T., Okada, T., Sakamoto, K., Takashima, M., Hamaguchi, T., Miyamoto, T., Uchimura, S., Hamamoto, Y., Yamada, M., Oka, M."Identification of ID2 associated with invasion of hepatitis C virus-related hepatocellular carcinoma by gene expression profile". International Journal of Oncology 29, no. 6 (2006): 1445-1451. https://doi.org/10.3892/ijo.29.6.1445