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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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June 2007 Volume 30 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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An International Open Access Journal Devoted to General Medicine.

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Article

Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB

  • Authors:
    • Kenji Negoro
    • Yukio Yamano
    • Kazuaki Fushimi
    • Kengo Saito
    • Ken Nakatani
    • Masashi Shiiba
    • Hidetaka Yokoe
    • Hiroki Bukawa
    • Katsuhiro Uzawa
    • Takeshi Wada
    • Hideki Tanzawa
    • Shigeyuki Fujita

  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, School of Medicine, Wakayama Medical University, Japan
  • Pages: 1325-1332
    |
    Published online on: June 1, 2007
       https://doi.org/10.3892/ijo.30.6.1325
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Abstract

To investigate the mechanism of the resistance to cisplatin (CDDP), we established the CDDP-resistant cell line, KB-R, from CDDP-sensitive oral carcinoma cell line, KB. The 3-(3, 4-dimethyl-thiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay indicated that KB-R is 5.5-fold more resistant to CDDP than KB. Microarray analysis indicated that the expression levels of 1,718 genes were elevated at least five-fold or more in KB-R, compared with KB. The expression status of ATP binding cassette (ABC) transporter genes, which belong to multi-drug resistance genes, was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction and real-time PCR. MRP1 and MRP2 were up-regulated, whereas MDR1 was down-regulated. Pathway and ontology analysis using the Ingenuity Pathway Analysis tool indicated three highly significant genetic networks including 105 of the 1,718 overexpressed genes and one network including 35 ‘cell-to-cell signaling and interaction’ related genes. Our results suggested that these cell lines, KB and KB-R, may be useful for searching the candidate genes responsible for CDDP-resistance and for further study to understand the mechanism of CDDP-resistance.

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Copy and paste a formatted citation
Spandidos Publications style
Negoro K, Yamano Y, Fushimi K, Saito K, Nakatani K, Shiiba M, Yokoe H, Bukawa H, Uzawa K, Wada T, Wada T, et al: Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB. Int J Oncol 30: 1325-1332, 2007.
APA
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M. ... Fujita, S. (2007). Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB. International Journal of Oncology, 30, 1325-1332. https://doi.org/10.3892/ijo.30.6.1325
MLA
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M., Yokoe, H., Bukawa, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB". International Journal of Oncology 30.6 (2007): 1325-1332.
Chicago
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M., Yokoe, H., Bukawa, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB". International Journal of Oncology 30, no. 6 (2007): 1325-1332. https://doi.org/10.3892/ijo.30.6.1325
Copy and paste a formatted citation
x
Spandidos Publications style
Negoro K, Yamano Y, Fushimi K, Saito K, Nakatani K, Shiiba M, Yokoe H, Bukawa H, Uzawa K, Wada T, Wada T, et al: Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB. Int J Oncol 30: 1325-1332, 2007.
APA
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M. ... Fujita, S. (2007). Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB. International Journal of Oncology, 30, 1325-1332. https://doi.org/10.3892/ijo.30.6.1325
MLA
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M., Yokoe, H., Bukawa, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB". International Journal of Oncology 30.6 (2007): 1325-1332.
Chicago
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M., Yokoe, H., Bukawa, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB". International Journal of Oncology 30, no. 6 (2007): 1325-1332. https://doi.org/10.3892/ijo.30.6.1325
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