Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB

  • Authors:
    • Kenji Negoro
    • Yukio Yamano
    • Kazuaki Fushimi
    • Kengo Saito
    • Ken Nakatani
    • Masashi Shiiba
    • Hidetaka Yokoe
    • Hiroki Bukawa
    • Katsuhiro Uzawa
    • Takeshi Wada
    • Hideki Tanzawa
    • Shigeyuki Fujita
  • View Affiliations

  • Published online on: June 1, 2007     https://doi.org/10.3892/ijo.30.6.1325
  • Pages: 1325-1332
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Abstract

To investigate the mechanism of the resistance to cisplatin (CDDP), we established the CDDP-resistant cell line, KB-R, from CDDP-sensitive oral carcinoma cell line, KB. The 3-(3, 4-dimethyl-thiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay indicated that KB-R is 5.5-fold more resistant to CDDP than KB. Microarray analysis indicated that the expression levels of 1,718 genes were elevated at least five-fold or more in KB-R, compared with KB. The expression status of ATP binding cassette (ABC) transporter genes, which belong to multi-drug resistance genes, was confirmed by semiquantitative reverse transcriptase-polymerase chain reaction and real-time PCR. MRP1 and MRP2 were up-regulated, whereas MDR1 was down-regulated. Pathway and ontology analysis using the Ingenuity Pathway Analysis tool indicated three highly significant genetic networks including 105 of the 1,718 overexpressed genes and one network including 35 ‘cell-to-cell signaling and interaction’ related genes. Our results suggested that these cell lines, KB and KB-R, may be useful for searching the candidate genes responsible for CDDP-resistance and for further study to understand the mechanism of CDDP-resistance.

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June 2007
Volume 30 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Negoro K, Yamano Y, Fushimi K, Saito K, Nakatani K, Shiiba M, Yokoe H, Bukawa H, Uzawa K, Wada T, Wada T, et al: Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB. Int J Oncol 30: 1325-1332, 2007.
APA
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M. ... Fujita, S. (2007). Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB. International Journal of Oncology, 30, 1325-1332. https://doi.org/10.3892/ijo.30.6.1325
MLA
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M., Yokoe, H., Bukawa, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB". International Journal of Oncology 30.6 (2007): 1325-1332.
Chicago
Negoro, K., Yamano, Y., Fushimi, K., Saito, K., Nakatani, K., Shiiba, M., Yokoe, H., Bukawa, H., Uzawa, K., Wada, T., Tanzawa, H., Fujita, S."Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB". International Journal of Oncology 30, no. 6 (2007): 1325-1332. https://doi.org/10.3892/ijo.30.6.1325