The inhibitory effect of the proinflammatory cytokine TNFα on erythroid differentiation involves erythroid transcription factor modulation

  • Authors:
    • Isabelle Buck
    • Franck Morceau
    • Silvia Cristofanon
    • Simone Reuter
    • Mario Dicato
    • Marc Diederich
  • View Affiliations

  • Published online on: March 1, 2009     https://doi.org/10.3892/ijo_00000212
  • Pages: 853-860
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Abstract

The hematopoietic transcription factor GATA-1 regulates the expression of several genes associated with differentiation of erythroid cells. We show here the inhibitory effect of tumor necrosis factor α (TNFα), a proinflammatory cytokine, on hemoglobinization and erythroid transcription factor GATA-1 expression in erythroleukemia (HEL) as well as in chronic myelogenous leukemia (K562) cells, which were induced to differentiate towards the erythroid lineage after aclacinomycin (Acla), doxorubicin (Dox) or hemin (HM) treatment. As a result, we observed i) a decreased expression of Friend of GATA-1 (FOG-1), an essential cofactor of GATA-1 transcription factor, ii) a downregulation of GATA-1 by proteasomal degradation and iii) a reduced acetylation level of GATA-1 in HM-induced K562 cells after TNF treatment. As a result, these modifications i) decreased the level of GATA-1/FOG-1 complex, ii) unsettled the GATA-1/GATA-2 balance, iii) reduced GATA-1 transcriptional activity and iv) inhibited erythroid marker gene expression (glycophorin A, erythropoietin receptor, γ-globin) independently of the cell line or the inducer used. These data provided new insights into the role of GATA-1 regulation in TNFα-mediated inhibition of erythroid differentiation in erythroleukemia.

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March 2009
Volume 34 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Buck I, Morceau F, Cristofanon S, Reuter S, Dicato M and Diederich M: The inhibitory effect of the proinflammatory cytokine TNFα on erythroid differentiation involves erythroid transcription factor modulation. Int J Oncol 34: 853-860, 2009
APA
Buck, I., Morceau, F., Cristofanon, S., Reuter, S., Dicato, M., & Diederich, M. (2009). The inhibitory effect of the proinflammatory cytokine TNFα on erythroid differentiation involves erythroid transcription factor modulation. International Journal of Oncology, 34, 853-860. https://doi.org/10.3892/ijo_00000212
MLA
Buck, I., Morceau, F., Cristofanon, S., Reuter, S., Dicato, M., Diederich, M."The inhibitory effect of the proinflammatory cytokine TNFα on erythroid differentiation involves erythroid transcription factor modulation". International Journal of Oncology 34.3 (2009): 853-860.
Chicago
Buck, I., Morceau, F., Cristofanon, S., Reuter, S., Dicato, M., Diederich, M."The inhibitory effect of the proinflammatory cytokine TNFα on erythroid differentiation involves erythroid transcription factor modulation". International Journal of Oncology 34, no. 3 (2009): 853-860. https://doi.org/10.3892/ijo_00000212