In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6

  • Authors:
    • Laura Monti
    • Raffaella Cinquetti
    • Alessandro Guffanti
    • Francesco Nicassio
    • Mattia Cremona
    • Giovanni Lavorgna
    • Fabrizio Bianchi
    • Francesca Vignati
    • Davide Cittaro
    • Roberto Taramelli
    • Francesco Acquati
  • View Affiliations

  • Published online on: April 1, 2009     https://doi.org/10.3892/ijo_00000237
  • Pages: 1099-1108
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Abstract

Antisense transcription has long been recognized as a mechanism involved in the regulation of gene expression. Therefore, several human diseases associated with abnormal patterns of gene expression might display antisense RNA-mediated pathogenetic mechanisms. Such issue could be particularly relevant for cancer pathogenesis, since deregulated gene expression has long been established as a hallmark of cancer cells. Herein, we report on a bioinformatic search for antisense transcription in two cancer-associated regions of human chromosome 6 (6q21 and 6q27). Natural antisense transcripts (NATs) for several genes in both genomic regions were predicted in silico and subsequently validated by strand-specific RT-PCR. Detailed experimental validation by quantitative real-time RT-PCR of five putative cancer related sense-antisense transcript pairs revealed a single candidate tumor suppressor gene (RPS6KA2) whose expression levels display marked cancer-related changes that are likely mediated by its antisense RNA in a breast cancer cell line model.

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April 2009
Volume 34 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Monti, L., Cinquetti, R., Guffanti, A., Nicassio, F., Cremona, M., Lavorgna, G. ... Acquati, F. (2009). In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6. International Journal of Oncology, 34, 1099-1108. https://doi.org/10.3892/ijo_00000237
MLA
Monti, L., Cinquetti, R., Guffanti, A., Nicassio, F., Cremona, M., Lavorgna, G., Bianchi, F., Vignati, F., Cittaro, D., Taramelli, R., Acquati, F."In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6". International Journal of Oncology 34.4 (2009): 1099-1108.
Chicago
Monti, L., Cinquetti, R., Guffanti, A., Nicassio, F., Cremona, M., Lavorgna, G., Bianchi, F., Vignati, F., Cittaro, D., Taramelli, R., Acquati, F."In silico prediction and experimental validation of natural antisense transcripts in two cancer-associated regions of human chromosome 6". International Journal of Oncology 34, no. 4 (2009): 1099-1108. https://doi.org/10.3892/ijo_00000237