Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases

  • Authors:
    • Francesca Perut
    • Elisabetta Cenni
    • Ronald E. Unger
    • C. James Kirkpatrick
    • Armando Giunti
    • Nicola Baldini
  • View Affiliations

  • Published online on: May 1, 2009     https://doi.org/10.3892/ijo_00000266
  • Pages: 1387-1393
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The immunogenic properties of renal cell carcinoma (RCC) on bone osteolysis were investigated. mRNA expression of three proinflammatory cytokines, monocyte chemotactic protein-1 (MCP-1), interleukin-6 (IL-6) and interleukin-8 (IL-8), were determined in a panel of RCC lines (CRBM 1990, ACHN and Caki-1). Moreover proinflammatory cytokine mRNA expression and protein levels of adhesion molecules, intercellular adhesion molecule-1 (ICAM-1) and E-selectin, on human umbilical vein endothelial cells (HUVEC) incubated with the conditioned media from RCC lines were evaluated. RCC express mRNA of MCP-1, IL-6 and IL-8 that may induce a proinflammatory phenotype in endothelial cells. mRNA expression of IL-6, and IL-8 was induced on HUVEC treated with the conditioned media from RCC lines and mRNA and protein levels of ICAM-1 and E-selectin were also increased. This study demonstrates the immunogenic properties of renal cell carcinoma, such as pro-inflammatory cytokine secretion and the induction of adhesion molecules (ICAM-1 and E-Sel) by endothelial cells. ICAM-1 binds lymphocyte function-associated antigen-1 (LFA-1), which is expressed by pre-osteoclasts, so that, the observed proinflammatory phenotype in HUVEC may also favour osteoclast recruitment in bone metastases microenvironment. Osteolysis in bone metastases, mediated by this pathway, may be further potentiated by the pro-angiogenic properties of RCC.

Related Articles

Journal Cover

May 2009
Volume 34 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Perut F, Cenni E, Unger RE, Kirkpatrick CJ, Giunti A and Baldini N: Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases. Int J Oncol 34: 1387-1393, 2009
APA
Perut, F., Cenni, E., Unger, R.E., Kirkpatrick, C.J., Giunti, A., & Baldini, N. (2009). Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases. International Journal of Oncology, 34, 1387-1393. https://doi.org/10.3892/ijo_00000266
MLA
Perut, F., Cenni, E., Unger, R. E., Kirkpatrick, C. J., Giunti, A., Baldini, N."Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases". International Journal of Oncology 34.5 (2009): 1387-1393.
Chicago
Perut, F., Cenni, E., Unger, R. E., Kirkpatrick, C. J., Giunti, A., Baldini, N."Immunogenic properties of renal cell carcinoma and the pathogenesis of osteolytic bone metastases". International Journal of Oncology 34, no. 5 (2009): 1387-1393. https://doi.org/10.3892/ijo_00000266