Se-methylselenocysteine sensitized TRAIL-mediated apoptosis via down-regulation of Bcl-2 expression

  • Authors:
    • Jung Tae Lee
    • Tae-Jin Lee
    • Jong-Wook Park
    • Taeg Kyu Kwon
  • View Affiliations

  • Published online on: May 1, 2009     https://doi.org/10.3892/ijo_00000274
  • Pages: 1455-1460
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Abstract

Recent studies establish a critical role of selenium in cancer prevention in vitro and in vivo. Selenium may sensitize TRAIL-mediated apoptosis in human renal cancer cells and increase therapeutic efficacy. In this study, we demonstrate that concomitant administration of TRAIL and Se-methylselenocysteine (Se-MSC) produces synergistic effects on the induction of apoptosis in Caki cells. Se-MSC rapidly and specifically down-regulates expression of the Bcl-2 at transcriptional level. The forced expression of Bcl-2 attenuated Se-MSC plus TRAIL-mediated apoptosis, suggesting that the lessened Bcl-2 expression caused by Se-MSC treatment is critical to the increased sensitivity to TRAIL in renal cancer cells. In addition, we demonstrate that the synergistic effects of Se-MSC and TRAIL result from the activation of the caspase-dependent pathways. Co-administration of HA14-1, a small molecule Bcl-2 inhibitor and TRAIL increased apoptosis in Caki cells. Taken together, Se-MSC-mediated down-regulation of Bcl-2 is able to sensitize Caki cells for TRAIL-induced apoptosis. Thus, selenium-based dietary compounds may help to overcome resistance to TRAIL-mediated apoptosis in renal cancer cells.

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May 2009
Volume 34 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lee JT, Lee T, Park J and Kwon TK: Se-methylselenocysteine sensitized TRAIL-mediated apoptosis via down-regulation of Bcl-2 expression. Int J Oncol 34: 1455-1460, 2009
APA
Lee, J.T., Lee, T., Park, J., & Kwon, T.K. (2009). Se-methylselenocysteine sensitized TRAIL-mediated apoptosis via down-regulation of Bcl-2 expression. International Journal of Oncology, 34, 1455-1460. https://doi.org/10.3892/ijo_00000274
MLA
Lee, J. T., Lee, T., Park, J., Kwon, T. K."Se-methylselenocysteine sensitized TRAIL-mediated apoptosis via down-regulation of Bcl-2 expression". International Journal of Oncology 34.5 (2009): 1455-1460.
Chicago
Lee, J. T., Lee, T., Park, J., Kwon, T. K."Se-methylselenocysteine sensitized TRAIL-mediated apoptosis via down-regulation of Bcl-2 expression". International Journal of Oncology 34, no. 5 (2009): 1455-1460. https://doi.org/10.3892/ijo_00000274