Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis

  • Authors:
    • Robert R. Langley
    • Dominic Fan
    • Lixia Guo
    • Chenyu Zhang
    • Qingtang Lin
    • Emily C. Brantley
    • Joseph H. McCarty
    • Isaiah J. Fidler
  • View Affiliations

  • Published online on: October 1, 2009     https://doi.org/10.3892/ijo_00000378
  • Pages: 665-672
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Abstract

Astrocytes play a critical role in maintaining cerebral homeostasis and their dysregulation is thought to contribute to the pathogenesis of several diseases, including brain cancer and metastasis. Similar to the human disease, we found that lung and melanoma metastases in the mouse brain are accompanied by a reactive gliosis. To begin to study the biology of astrocytes and examine how these cells might contribute to metastasis formation and progression in the brain, we generated a conditionally immortal astrocyte cell line from H-2Kb-tsA58 mice. Astrocytes grown in culture expressed glial fibrillary acid protein (GFAP), glutamate receptor 1, and the N-methyl-D-aspartate (NMDA) receptor. Astrocytes also expressed the glial-specific transporters excitatory amino acid transporter 1 (EAAT1) and EAAT2. Astrocytes grown under permissive conditions (33°C) expressed SV40 large T antigen and had a doubling time of 36 h, whereas expression of SV40 large T antigen was negligible in astrocytes grown at 37°C for 72 h, which coincided with a plateau in cell division. In a co-culture assay with human lung adenocarcinoma cells (PC14-PE6), astrocytes activated programs in the tumor cells that signal for cell division and survival. Hence, the immortalized cell line will be useful for studying the role of astrocytes in disease processes in the brain, such as metastasis.

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October 2009
Volume 35 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Langley RR, Fan D, Guo L, Zhang C, Lin Q, Brantley EC, McCarty JH and Fidler IJ: Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis. Int J Oncol 35: 665-672, 2009
APA
Langley, R.R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E.C. ... Fidler, I.J. (2009). Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis. International Journal of Oncology, 35, 665-672. https://doi.org/10.3892/ijo_00000378
MLA
Langley, R. R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E. C., McCarty, J. H., Fidler, I. J."Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis". International Journal of Oncology 35.4 (2009): 665-672.
Chicago
Langley, R. R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E. C., McCarty, J. H., Fidler, I. J."Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis". International Journal of Oncology 35, no. 4 (2009): 665-672. https://doi.org/10.3892/ijo_00000378