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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2009 Volume 35 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2009 Volume 35 Issue 4

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Article

Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis

  • Authors:
    • Robert R. Langley
    • Dominic Fan
    • Lixia Guo
    • Chenyu Zhang
    • Qingtang Lin
    • Emily C. Brantley
    • Joseph H. McCarty
    • Isaiah J. Fidler

  • View Affiliations / Copyright

    Affiliations: Department of Cancer Biology, Unit 173, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA. rlangley@mdanderson.org
  • Pages: 665-672
    |
    Published online on: October 1, 2009
       https://doi.org/10.3892/ijo_00000378
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Abstract

Astrocytes play a critical role in maintaining cerebral homeostasis and their dysregulation is thought to contribute to the pathogenesis of several diseases, including brain cancer and metastasis. Similar to the human disease, we found that lung and melanoma metastases in the mouse brain are accompanied by a reactive gliosis. To begin to study the biology of astrocytes and examine how these cells might contribute to metastasis formation and progression in the brain, we generated a conditionally immortal astrocyte cell line from H-2Kb-tsA58 mice. Astrocytes grown in culture expressed glial fibrillary acid protein (GFAP), glutamate receptor 1, and the N-methyl-D-aspartate (NMDA) receptor. Astrocytes also expressed the glial-specific transporters excitatory amino acid transporter 1 (EAAT1) and EAAT2. Astrocytes grown under permissive conditions (33°C) expressed SV40 large T antigen and had a doubling time of 36 h, whereas expression of SV40 large T antigen was negligible in astrocytes grown at 37°C for 72 h, which coincided with a plateau in cell division. In a co-culture assay with human lung adenocarcinoma cells (PC14-PE6), astrocytes activated programs in the tumor cells that signal for cell division and survival. Hence, the immortalized cell line will be useful for studying the role of astrocytes in disease processes in the brain, such as metastasis.

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Copy and paste a formatted citation
Spandidos Publications style
Langley RR, Fan D, Guo L, Zhang C, Lin Q, Brantley EC, McCarty JH and Fidler IJ: Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis. Int J Oncol 35: 665-672, 2009.
APA
Langley, R.R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E.C. ... Fidler, I.J. (2009). Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis. International Journal of Oncology, 35, 665-672. https://doi.org/10.3892/ijo_00000378
MLA
Langley, R. R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E. C., McCarty, J. H., Fidler, I. J."Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis". International Journal of Oncology 35.4 (2009): 665-672.
Chicago
Langley, R. R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E. C., McCarty, J. H., Fidler, I. J."Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis". International Journal of Oncology 35, no. 4 (2009): 665-672. https://doi.org/10.3892/ijo_00000378
Copy and paste a formatted citation
x
Spandidos Publications style
Langley RR, Fan D, Guo L, Zhang C, Lin Q, Brantley EC, McCarty JH and Fidler IJ: Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis. Int J Oncol 35: 665-672, 2009.
APA
Langley, R.R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E.C. ... Fidler, I.J. (2009). Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis. International Journal of Oncology, 35, 665-672. https://doi.org/10.3892/ijo_00000378
MLA
Langley, R. R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E. C., McCarty, J. H., Fidler, I. J."Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis". International Journal of Oncology 35.4 (2009): 665-672.
Chicago
Langley, R. R., Fan, D., Guo, L., Zhang, C., Lin, Q., Brantley, E. C., McCarty, J. H., Fidler, I. J."Generation of an immortalized astrocyte cell line from H-2Kb-tsA58 mice to study the role of astrocytes in brain metastasis". International Journal of Oncology 35, no. 4 (2009): 665-672. https://doi.org/10.3892/ijo_00000378
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