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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2009 Volume 35 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 2009 Volume 35 Issue 4

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Article

In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein

  • Authors:
    • Lori Holle
    • Wen Song
    • Eric Holle
    • Yangzhang Wei
    • Jinhua Li
    • Thomas E. Wagner
    • Xianzhong Yu

  • View Affiliations / Copyright

    Affiliations: Oncology Research Institute of the Greenville Hospital System, Greenville, SC 29605, USA
  • Pages: 829-835
    |
    Published online on: October 1, 2009
       https://doi.org/10.3892/ijo_00000396
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Abstract

Chemotherapy is one of the main treatment options for cancer, but the effectiveness of chemotherapeutic drugs is severely limited due to their systemic toxicity. Therefore, the need for a more targeted approach in tumor treatment is obvious. A tumor-activated agent would decrease systemic toxicity as well as increase the efficacy of the treatment. It has previously been shown that the latency of pro-TGF-β is conferred by dimerization of two latency-associated peptides (LAP) that form a protective shield, which is cleaved off upon activation by matrix metalloproteinases (MMPs). It has also been shown that the fusion of this LAP peptide with other cytokines can confer their latency. In the present study, a recombinant adenovirus with a fusion gene encoding a tumor-activated pro-cytolytic peptide was made in which the LAP domain of TGF-β was fused with melittin, a potent cytolytic toxin, with an MMP2 cleavage site in between the two. In vitro studies show that the melittin-MMP2-LAP recombinant adenovirus can be activated by MMP2 which leads to the release of free melittin to lyse the target cells. In vivo studies show approximately a 70% decrease in B16 tumor volume in melittin-MMP2-LAP recombinant adenovirus-treated mice as compared to control mice. No significant systemic toxicity was observed in the treated mice.

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Copy and paste a formatted citation
Spandidos Publications style
Holle L, Song W, Holle E, Wei Y, Li J, Wagner TE and Yu X: In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein. Int J Oncol 35: 829-835, 2009.
APA
Holle, L., Song, W., Holle, E., Wei, Y., Li, J., Wagner, T.E., & Yu, X. (2009). In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein. International Journal of Oncology, 35, 829-835. https://doi.org/10.3892/ijo_00000396
MLA
Holle, L., Song, W., Holle, E., Wei, Y., Li, J., Wagner, T. E., Yu, X."In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein". International Journal of Oncology 35.4 (2009): 829-835.
Chicago
Holle, L., Song, W., Holle, E., Wei, Y., Li, J., Wagner, T. E., Yu, X."In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein". International Journal of Oncology 35, no. 4 (2009): 829-835. https://doi.org/10.3892/ijo_00000396
Copy and paste a formatted citation
x
Spandidos Publications style
Holle L, Song W, Holle E, Wei Y, Li J, Wagner TE and Yu X: In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein. Int J Oncol 35: 829-835, 2009.
APA
Holle, L., Song, W., Holle, E., Wei, Y., Li, J., Wagner, T.E., & Yu, X. (2009). In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein. International Journal of Oncology, 35, 829-835. https://doi.org/10.3892/ijo_00000396
MLA
Holle, L., Song, W., Holle, E., Wei, Y., Li, J., Wagner, T. E., Yu, X."In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein". International Journal of Oncology 35.4 (2009): 829-835.
Chicago
Holle, L., Song, W., Holle, E., Wei, Y., Li, J., Wagner, T. E., Yu, X."In vitro- and in vivo-targeted tumor lysis by an MMP2 cleavable melittin-LAP fusion protein". International Journal of Oncology 35, no. 4 (2009): 829-835. https://doi.org/10.3892/ijo_00000396
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