Cell density-dependent regulation of p73 in breast cancer cells

Tophkhane,Chaitali; Yang,Shihe; Zhao,Zhizhuang Joe; Yang,Xiaohe

doi:10.3892/ijo_00000461

December 2009 Volume 35 Issue 6

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December 2009 Volume 35 Issue 6

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Article

Cell density-dependent regulation of p73 in breast cancer cells

Authors:
- Chaitali Tophkhane
- Shihe Yang
- Zhizhuang Joe Zhao
- Xiaohe Yang
View Affiliations / Copyright

Affiliations: Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
Pages: 1429-1434
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Published online on: December 1, 2009

https://doi.org/10.3892/ijo_00000461
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Abstract

Molecular regulation of p73, a p53 family member, remains unclear. Here we report that p73 expression is significantly regulated by cell densities. In particular, we found that p73α and p73β are differentially regulated. While p73β protein levels were inversely correlated with cell densities, p73α protein levels behaved oppositely. We further showed that density-dependent changes of p73α follow the same patterns as E2F-1 and TAp73 mRNA levels, suggesting transcriptional regulation. Our data also suggest that high levels of p73β at lower densities may be due to increased protein stability. However, AIP-4/Itch appeared not to be involved in downregulation of p73β at high densities. Moreover, we also found that subcellular location of p73 isoforms changes with the culture density increases. While high level of p73β at low density was mainly presented in the nucleus, low levels of this protein at high densities were mainly in the cytosol. Taken together, these findings reveal a novel mechanism that differentially regulates p73 isoforms and underscores the role of cell-cell interaction in p73 regulation, which may advance our understanding of p73 expression and function in human cancers.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Cell density-dependent regulation of p73 in breast cancer cells

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Abstract

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