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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May 2010 Volume 36 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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May 2010 Volume 36 Issue 5

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Article

A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth

  • Authors:
    • Yang Wan
    • Anliang Huang
    • Yang Yang
    • Gang Xie
    • Xiang Chen
    • Jia Hu
    • Xiancheng Chen
    • Li Yang
    • Jiong Li
    • Lijuan Chen
    • Yu Jiang
    • Xia Zhao
    • Yuquan Wei
    • Hongxin Deng

  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Biotherapy, West China Hospital and West China Medical School, Sichuan University, Sichuan, 610041, P.R. China
  • Pages: 1121-1128
    |
    Published online on: May 1, 2010
       https://doi.org/10.3892/ijo_00000594
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Abstract

Aurora A plays an essential role in centrosome maturation, separation and in the formation of the mitotic bipolar spindle. Overexpression or amplication of Aurora A gene has been detected in many cancer cell lines and various tumor tissues, including breast cancer, suggesting that Aurora A might be drug target for breast cancer treatment. In the current study, short hairpin RNA targeting Aurora A was cloned into pGenesil-2 plasmid vector and then transfected into MDA-MB-435S and ZR-75-30 human breast cancer cells using cationic liposome. Reduced expression of Aurora A was detected by RT-PCR and Western blot. The effect of pGenesil-2-shAURKA plasmid on tumor growth in MDA-MB-435S xenogenic implantation model was studied. pGenesil-2-shAURKA plasmid inhibited tumor growth significantly by systemantic administration. To further study the underlying mechanisms, cell apoptosis and proliferation were investigated by flow cytometric analysis, propidium iodide staining, TUNEL and Ki-67 immunostaining respectively. Increased apoptosis and reduced cell proliferation were detected in vitro and in vivo studies. In summary, our results suggested that specific knockdown of Aurora A expression by vector based shRNA may be a potential therapy for human breast cancer.

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Copy and paste a formatted citation
Spandidos Publications style
Wan Y, Huang A, Yang Y, Xie G, Chen X, Hu J, Chen X, Yang L, Li J, Chen L, Chen L, et al: A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth. Int J Oncol 36: 1121-1128, 2010.
APA
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J. ... Deng, H. (2010). A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth. International Journal of Oncology, 36, 1121-1128. https://doi.org/10.3892/ijo_00000594
MLA
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J., Chen, X., Yang, L., Li, J., Chen, L., Jiang, Y., Zhao, X., Wei, Y., Deng, H."A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth". International Journal of Oncology 36.5 (2010): 1121-1128.
Chicago
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J., Chen, X., Yang, L., Li, J., Chen, L., Jiang, Y., Zhao, X., Wei, Y., Deng, H."A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth". International Journal of Oncology 36, no. 5 (2010): 1121-1128. https://doi.org/10.3892/ijo_00000594
Copy and paste a formatted citation
x
Spandidos Publications style
Wan Y, Huang A, Yang Y, Xie G, Chen X, Hu J, Chen X, Yang L, Li J, Chen L, Chen L, et al: A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth. Int J Oncol 36: 1121-1128, 2010.
APA
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J. ... Deng, H. (2010). A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth. International Journal of Oncology, 36, 1121-1128. https://doi.org/10.3892/ijo_00000594
MLA
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J., Chen, X., Yang, L., Li, J., Chen, L., Jiang, Y., Zhao, X., Wei, Y., Deng, H."A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth". International Journal of Oncology 36.5 (2010): 1121-1128.
Chicago
Wan, Y., Huang, A., Yang, Y., Xie, G., Chen, X., Hu, J., Chen, X., Yang, L., Li, J., Chen, L., Jiang, Y., Zhao, X., Wei, Y., Deng, H."A vector-based short hairpin RNA targeting Aurora A inhibits breast cancer growth". International Journal of Oncology 36, no. 5 (2010): 1121-1128. https://doi.org/10.3892/ijo_00000594
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