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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 2010 Volume 37 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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October 2010 Volume 37 Issue 4

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Article

Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma

  • Authors:
    • Jun Nakamura
    • Naohiko Kohya
    • Keita Kai
    • Kazuma Ohtaka
    • Kazuyoshi Hashiguchi
    • Masatsugu Hiraki
    • Yoshihiko Kitajima
    • Osamu Tokunaga
    • Hirokazu Noshiro
    • Kohji Miyazaki

  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Saga University Faculty of Medicine, Saga 849-8501, Japan
  • Pages: 845-852
    |
    Published online on: October 1, 2010
       https://doi.org/10.3892/ijo_00000735
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Abstract

Gemcitabine is a commonly used chemotherapeutic agent for advanced biliary tract carcinoma (BTC), although its efficacy is insufficient. Therefore, it is essential to establish new diagnostic methods, which can predict responders before the treatment. The aim of this study is to identify the most reliable chemoresistance marker to gemcitabine in BTC among the 4 molecules (hENT1, dCK, RRM1 and RRM2) involved in gemcitabine metabolism. The expression of 4 molecules were investigated in 5 BTC cell lines, and correlated with gemcitabine sensitivity. RRM1 protein was also assessed by quantitative double-fluorescence immunohistochemistry (qDFIHC) in 10 patients with unresectable or recurrent BTC who received gemcitabine-based chemotherapy. RRM1 and RRM2 protein strongly correlated with the IC50 value for gemcitabine in BTC cell lines (R=0.935, 0.771, respectively). In addition, patients with low RRM1 were significantly more sensitive to gemcitabine (p=0.033), and their survival was significantly better than patients with high RRM1 (p=0.001). In conclusion, RRM1 particularly in protein level is a reliable marker for gemcitabine resistance in BTC. Furthermore, qDFIHC is a useful method for the assessment of RRM1 protein, in order to design a tailor-made chemotherapeutic regimen for BTC patients.

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Copy and paste a formatted citation
Spandidos Publications style
Nakamura J, Kohya N, Kai K, Ohtaka K, Hashiguchi K, Hiraki M, Kitajima Y, Tokunaga O, Noshiro H, Miyazaki K, Miyazaki K, et al: Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma. Int J Oncol 37: 845-852, 2010.
APA
Nakamura, J., Kohya, N., Kai, K., Ohtaka, K., Hashiguchi, K., Hiraki, M. ... Miyazaki, K. (2010). Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma. International Journal of Oncology, 37, 845-852. https://doi.org/10.3892/ijo_00000735
MLA
Nakamura, J., Kohya, N., Kai, K., Ohtaka, K., Hashiguchi, K., Hiraki, M., Kitajima, Y., Tokunaga, O., Noshiro, H., Miyazaki, K."Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma". International Journal of Oncology 37.4 (2010): 845-852.
Chicago
Nakamura, J., Kohya, N., Kai, K., Ohtaka, K., Hashiguchi, K., Hiraki, M., Kitajima, Y., Tokunaga, O., Noshiro, H., Miyazaki, K."Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma". International Journal of Oncology 37, no. 4 (2010): 845-852. https://doi.org/10.3892/ijo_00000735
Copy and paste a formatted citation
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Spandidos Publications style
Nakamura J, Kohya N, Kai K, Ohtaka K, Hashiguchi K, Hiraki M, Kitajima Y, Tokunaga O, Noshiro H, Miyazaki K, Miyazaki K, et al: Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma. Int J Oncol 37: 845-852, 2010.
APA
Nakamura, J., Kohya, N., Kai, K., Ohtaka, K., Hashiguchi, K., Hiraki, M. ... Miyazaki, K. (2010). Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma. International Journal of Oncology, 37, 845-852. https://doi.org/10.3892/ijo_00000735
MLA
Nakamura, J., Kohya, N., Kai, K., Ohtaka, K., Hashiguchi, K., Hiraki, M., Kitajima, Y., Tokunaga, O., Noshiro, H., Miyazaki, K."Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma". International Journal of Oncology 37.4 (2010): 845-852.
Chicago
Nakamura, J., Kohya, N., Kai, K., Ohtaka, K., Hashiguchi, K., Hiraki, M., Kitajima, Y., Tokunaga, O., Noshiro, H., Miyazaki, K."Ribonucleotide reductase subunit M1 assessed by quantitative double-fluorescence immunohistochemistry predicts the efficacy of gemcitabine in biliary tract carcinoma". International Journal of Oncology 37, no. 4 (2010): 845-852. https://doi.org/10.3892/ijo_00000735
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