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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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November 2010 Volume 37 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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November 2010 Volume 37 Issue 5

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Article Open Access

Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis

  • Authors:
    • Kosei Kimura
    • Akira Wada
    • Masami Ueta
    • Akihiko Ogata
    • Satoru Tanaka
    • Akiko Sakai
    • Hideji Yoshida
    • Hideo Fushitani
    • Akiko Miyamoto
    • Masakazu Fukushima
    • Toshio Uchiumi
    • Nobuhiko Tanigawa

  • View Affiliations / Copyright

    Affiliations: Department of General and Gastroenterological Surgery, Osaka Medical College, Takatsuki City, Osaka 569-8686, Japan
  • Pages: 1271-1278
    |
    Published online on: November 1, 2010
       https://doi.org/10.3892/ijo_00000778
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Abstract

Many auxiliary functions of ribosomal proteins (r-proteins) have received considerable attention in recent years. However, human r-proteins have hardly been examined by proteomic analysis. In this study, we isolated ribosomal particles and subsequently compared the proteome of r-proteins between the DLD-1 human colon cancer cell line and its 5-fluorouracil (5-FU)-resistant sub-line, DLD-1/5-FU, using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis, which has a superior ability to separate basic proteins, and we discuss the role of r-proteins in 5-FU resistance. Densitometric analysis was performed to quantify modulated proteins, and protein spots showing significant changes were identified by employing matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Three basic proteins (L15, L37 and prohibitin) which were significantly modulated between DLD-1 and DLD-1/5-FU were identified. Two proteins, L15 and L37, showed down-regulated expression in DLD-1/5-FU in comparison to DLD-1. Prohibitin, which is not an r-protein and is known to be localized in the mitochondria, showed up-regulated expression in DLD-1/5-FU. These 3 proteins may be related to 5-FU resistance.

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Copy and paste a formatted citation
Spandidos Publications style
Kimura K, Wada A, Ueta M, Ogata A, Tanaka S, Sakai A, Yoshida H, Fushitani H, Miyamoto A, Fukushima M, Fukushima M, et al: Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. Int J Oncol 37: 1271-1278, 2010.
APA
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A. ... Tanigawa, N. (2010). Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. International Journal of Oncology, 37, 1271-1278. https://doi.org/10.3892/ijo_00000778
MLA
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A., Yoshida, H., Fushitani, H., Miyamoto, A., Fukushima, M., Uchiumi, T., Tanigawa, N."Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis". International Journal of Oncology 37.5 (2010): 1271-1278.
Chicago
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A., Yoshida, H., Fushitani, H., Miyamoto, A., Fukushima, M., Uchiumi, T., Tanigawa, N."Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis". International Journal of Oncology 37, no. 5 (2010): 1271-1278. https://doi.org/10.3892/ijo_00000778
Copy and paste a formatted citation
x
Spandidos Publications style
Kimura K, Wada A, Ueta M, Ogata A, Tanaka S, Sakai A, Yoshida H, Fushitani H, Miyamoto A, Fukushima M, Fukushima M, et al: Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. Int J Oncol 37: 1271-1278, 2010.
APA
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A. ... Tanigawa, N. (2010). Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. International Journal of Oncology, 37, 1271-1278. https://doi.org/10.3892/ijo_00000778
MLA
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A., Yoshida, H., Fushitani, H., Miyamoto, A., Fukushima, M., Uchiumi, T., Tanigawa, N."Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis". International Journal of Oncology 37.5 (2010): 1271-1278.
Chicago
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A., Yoshida, H., Fushitani, H., Miyamoto, A., Fukushima, M., Uchiumi, T., Tanigawa, N."Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis". International Journal of Oncology 37, no. 5 (2010): 1271-1278. https://doi.org/10.3892/ijo_00000778
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