Open Access

Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis

  • Authors:
    • Kosei Kimura
    • Akira Wada
    • Masami Ueta
    • Akihiko Ogata
    • Satoru Tanaka
    • Akiko Sakai
    • Hideji Yoshida
    • Hideo Fushitani
    • Akiko Miyamoto
    • Masakazu Fukushima
    • Toshio Uchiumi
    • Nobuhiko Tanigawa
  • View Affiliations

  • Published online on: November 1, 2010     https://doi.org/10.3892/ijo_00000778
  • Pages: 1271-1278
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Abstract

Many auxiliary functions of ribosomal proteins (r-proteins) have received considerable attention in recent years. However, human r-proteins have hardly been examined by proteomic analysis. In this study, we isolated ribosomal particles and subsequently compared the proteome of r-proteins between the DLD-1 human colon cancer cell line and its 5-fluorouracil (5-FU)-resistant sub-line, DLD-1/5-FU, using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis, which has a superior ability to separate basic proteins, and we discuss the role of r-proteins in 5-FU resistance. Densitometric analysis was performed to quantify modulated proteins, and protein spots showing significant changes were identified by employing matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Three basic proteins (L15, L37 and prohibitin) which were significantly modulated between DLD-1 and DLD-1/5-FU were identified. Two proteins, L15 and L37, showed down-regulated expression in DLD-1/5-FU in comparison to DLD-1. Prohibitin, which is not an r-protein and is known to be localized in the mitochondria, showed up-regulated expression in DLD-1/5-FU. These 3 proteins may be related to 5-FU resistance.

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November 2010
Volume 37 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kimura K, Wada A, Ueta M, Ogata A, Tanaka S, Sakai A, Yoshida H, Fushitani H, Miyamoto A, Fukushima M, Fukushima M, et al: Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. Int J Oncol 37: 1271-1278, 2010
APA
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A. ... Tanigawa, N. (2010). Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. International Journal of Oncology, 37, 1271-1278. https://doi.org/10.3892/ijo_00000778
MLA
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A., Yoshida, H., Fushitani, H., Miyamoto, A., Fukushima, M., Uchiumi, T., Tanigawa, N."Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis". International Journal of Oncology 37.5 (2010): 1271-1278.
Chicago
Kimura, K., Wada, A., Ueta, M., Ogata, A., Tanaka, S., Sakai, A., Yoshida, H., Fushitani, H., Miyamoto, A., Fukushima, M., Uchiumi, T., Tanigawa, N."Comparative proteomic analysis of the ribosomes in 5-fluorouracil resistance of a human colon cancer cell line using the radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis". International Journal of Oncology 37, no. 5 (2010): 1271-1278. https://doi.org/10.3892/ijo_00000778