Evaluation of the effectiveness of a chemoprevention model of pancreatic adenocarcinoma using protein chip technology

  • Authors:
    • Liping Cao
    • Liang Wang
    • Guifeng Wang
    • Daren Liu
    • Jiekai Yu
    • Risheng Que
    • Dafei Xie
  • View Affiliations

  • Published online on: December 1, 2010     https://doi.org/10.3892/ijo_00000804
  • Pages: 1515-1520
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Abstract

A serum-specific protein ‘fingerprint’ model was established which is capable of evaluating the effect of chemotherapy (gemcitabine) of pancreatic adenocarcinoma. We used SELDI-TOF-MS coupled with CM10 chips and bioinformatics tools to analyze a total of 45 mouse serum samples from three groups: the healthy control group, the pancreatic cancer model group (orthotopic transplantation model of human pancreatic adenocarcinoma) and the gemcitabine-treated group to establish diagnostic models. As a result, the test set yielded a specificity of 95.0% and a sensitivity of 95.0% for pattern 1, which distinguished pancreatic adenocarcinoma from healthy individuals and a specificity of 95.0% and a sensitivity of 75.0% for pattern 3, which distinguished healthy controls, PC model group and gemcitabine-treated group, as evaluated by leave-one-out cross-validation. We concluded from this study that the SELDI-TOF-MS technique combined with bioinformatics approaches can facilitate evaluating the effect of chemotherapy (gemcitabine) for pancreatic adenocarcinoma and could be used as a potential prognostic monitoring method.

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December 2010
Volume 37 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Cao L, Wang L, Wang G, Liu D, Yu J, Que R and Xie D: Evaluation of the effectiveness of a chemoprevention model of pancreatic adenocarcinoma using protein chip technology. Int J Oncol 37: 1515-1520, 2010
APA
Cao, L., Wang, L., Wang, G., Liu, D., Yu, J., Que, R., & Xie, D. (2010). Evaluation of the effectiveness of a chemoprevention model of pancreatic adenocarcinoma using protein chip technology. International Journal of Oncology, 37, 1515-1520. https://doi.org/10.3892/ijo_00000804
MLA
Cao, L., Wang, L., Wang, G., Liu, D., Yu, J., Que, R., Xie, D."Evaluation of the effectiveness of a chemoprevention model of pancreatic adenocarcinoma using protein chip technology". International Journal of Oncology 37.6 (2010): 1515-1520.
Chicago
Cao, L., Wang, L., Wang, G., Liu, D., Yu, J., Que, R., Xie, D."Evaluation of the effectiveness of a chemoprevention model of pancreatic adenocarcinoma using protein chip technology". International Journal of Oncology 37, no. 6 (2010): 1515-1520. https://doi.org/10.3892/ijo_00000804