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Article

Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo

  • Authors:
    • Ji-Ye Kee
    • Yoshihisa Arita
    • Kanna Shinohara
    • Yasukata Ohashi
    • Hiroaki Sakurai
    • Ikuo Saiki
    • Keiichi Koizumi
  • View Affiliations / Copyright

    Affiliations: Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan, Department of Cancer Cell Biology, Graduate School Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan, Division of Kampo Diagnostics, Institute of Natural Medicine, University of Toyama, Toyama 930-0194, Japan
  • Pages: 35-40
    |
    Published online on: September 28, 2012
       https://doi.org/10.3892/mco.2012.30
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Abstract

Due to their chemoattractant properties stimulating the accumulation of infiltrating immune cells in tumors, chemokines are known to have antitumor effects. Fractalkine, a unique CX3C chemokine, is expressed in activated endothelial cells, while its receptor, CX3CR1, is expressed in cytolytic immune cells, such as natural killer cells, monocytes and some CD8+ T cells. The biological properties of cancer cells are affected by the implantation organ and differences in immune systems, requiring cancer implantation in orthotopic organs in an in vivo experiment. To develop new therapy strategies for lung cancer, an animal model reflecting the clinical features of lung cancer was previously established. This study aimed to determine whether CX3CL1-induced biological functions should be used for immune cell-based gene therapy of lung cancer in the orthotopic implantation model. An orthotopic intrapulmonary implantation of CX3CL1-stable expression in mouse lung cancer (LLC-CX3CL1) was performed to analyze growth. Results showed a significant decrease in tumor growth in the lung compared to the control cells (LLC-mock). Furthermore, the antitumor effects of CX3CL1 were derived from natural killer cell activities in the depletion experiment in vivo. Therefore, CX3CL1 has the potential of a useful therapeutic target in lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kee J, Arita Y, Shinohara K, Ohashi Y, Sakurai H, Saiki I and Koizumi K: Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo. Mol Clin Oncol 1: 35-40, 2013.
APA
Kee, J., Arita, Y., Shinohara, K., Ohashi, Y., Sakurai, H., Saiki, I., & Koizumi, K. (2013). Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo. Molecular and Clinical Oncology, 1, 35-40. https://doi.org/10.3892/mco.2012.30
MLA
Kee, J., Arita, Y., Shinohara, K., Ohashi, Y., Sakurai, H., Saiki, I., Koizumi, K."Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo". Molecular and Clinical Oncology 1.1 (2013): 35-40.
Chicago
Kee, J., Arita, Y., Shinohara, K., Ohashi, Y., Sakurai, H., Saiki, I., Koizumi, K."Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo". Molecular and Clinical Oncology 1, no. 1 (2013): 35-40. https://doi.org/10.3892/mco.2012.30
Copy and paste a formatted citation
x
Spandidos Publications style
Kee J, Arita Y, Shinohara K, Ohashi Y, Sakurai H, Saiki I and Koizumi K: Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo. Mol Clin Oncol 1: 35-40, 2013.
APA
Kee, J., Arita, Y., Shinohara, K., Ohashi, Y., Sakurai, H., Saiki, I., & Koizumi, K. (2013). Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo. Molecular and Clinical Oncology, 1, 35-40. https://doi.org/10.3892/mco.2012.30
MLA
Kee, J., Arita, Y., Shinohara, K., Ohashi, Y., Sakurai, H., Saiki, I., Koizumi, K."Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo". Molecular and Clinical Oncology 1.1 (2013): 35-40.
Chicago
Kee, J., Arita, Y., Shinohara, K., Ohashi, Y., Sakurai, H., Saiki, I., Koizumi, K."Antitumor immune activity by chemokine CX3CL1 in an orthotopic implantation of lung cancer model in vivo". Molecular and Clinical Oncology 1, no. 1 (2013): 35-40. https://doi.org/10.3892/mco.2012.30
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