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Article

Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion

  • Authors:
    • Jing Chen
    • Na Li
    • Gongchu Li
  • View Affiliations / Copyright

    Affiliations: College of Life Sciences, Zhejiang Sci-Tech University, Zhejiang 310018, P.R. China
  • Pages: 483-486
    |
    Published online on: January 29, 2013
       https://doi.org/10.3892/mco.2013.73
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Abstract

The liver is a frequent site for the metastasis of cancer cells originating from other sites. Leukemic liver metastasis is associated with poor prognosis. The ligation of CD44 with hyaluronan (HA) has been shown to contribute to the drug resistance of leukemic cells. In this study, a link protein HA‑binding motif was genetically fused with enhanced green fluorescence protein (EGFP) to generate an EGFP-L fusion protein. Furthermore, a coculture system was established to investigate the interaction of leukemic cells with liver cells. CD44‑positive Kasumi‑1, but not CD44-negative HL-60 cells, were observed to adhere to the liver cell line L02. This cell‑cell adhesion was significantly blocked by HA, indicating that Kasumi-L02 cell adhesion was mediated by the CD44‑HA interaction. Compared to EGFP, EGFP-L fusion protein bound to L02 and BEL7404 liver cells. EGFP-L partially abrogated the Kasumi-L02 adhesion, suggesting that the link protein‑binding motif is able to inhibit CD44‑HA‑mediated leukemia‑liver adhesion. These results may help provide insight into novel therapeutic methods for leukemic patients diagnosed with liver metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen J, Li N and Li G: Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion. Mol Clin Oncol 1: 483-486, 2013.
APA
Chen, J., Li, N., & Li, G. (2013). Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion. Molecular and Clinical Oncology, 1, 483-486. https://doi.org/10.3892/mco.2013.73
MLA
Chen, J., Li, N., Li, G."Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion". Molecular and Clinical Oncology 1.3 (2013): 483-486.
Chicago
Chen, J., Li, N., Li, G."Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion". Molecular and Clinical Oncology 1, no. 3 (2013): 483-486. https://doi.org/10.3892/mco.2013.73
Copy and paste a formatted citation
x
Spandidos Publications style
Chen J, Li N and Li G: Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion. Mol Clin Oncol 1: 483-486, 2013.
APA
Chen, J., Li, N., & Li, G. (2013). Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion. Molecular and Clinical Oncology, 1, 483-486. https://doi.org/10.3892/mco.2013.73
MLA
Chen, J., Li, N., Li, G."Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion". Molecular and Clinical Oncology 1.3 (2013): 483-486.
Chicago
Chen, J., Li, N., Li, G."Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion". Molecular and Clinical Oncology 1, no. 3 (2013): 483-486. https://doi.org/10.3892/mco.2013.73
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