Link protein hyaluronan‑binding motif abrogates CD44‑hyaluronan‑mediated leukemia-liver cell adhesion

Introduction

The liver is a frequent site for the metastasis of cancer cells originating from other sites. A significant number of leukemic or lymphoma patients have liver metastasis (1). Notably, liver enlargement with malignant infiltration in leukemic and lymphoma patients is associated with poor prognosis (2). Inhibition of the interaction between liver cells and infiltrated malignant cells may provide a novel therapeutic method for patients diagnosed with leukemic liver metastasis.

The CD44 transmembrane glycoprotein is ubiquitously expressed in cells and tissues and is involved in inflammation (3) and tumorigenesis (4). Due to alternative splicing of various exons, CD44 is usually expressed in a variety of isoforms (CD44v) (5). The CD44 isoforms contain a hyaluronan (HA)-binding domain in the extracellular domain (6). HA is a major component of the extracellular matrix (ECM), and interacts with a variety of ECM and cell membrane proteins (6–10), among which link protein is a component of ECM interacting with HA through its HA-binding motif (16). As an important cell adhesion molecule, CD44 mediates cell-cell and cell-ECM interaction, inducing intracellular signaling events (11,12). Notably, the ligation of CD44 has been shown to induce the activation or expression of intracellular signaling elements, such as MDR1, phosphoinositide 3 kinase (PI3K) (13), Lyn (14), Ca2+ mobilization (15) and ERK (16), which are involved in apoptosis inhibition and chemoresistance in tumors.

In this study, an in vitro coculture system of leukemic and liver cells was established, determined to be mediated by the interaction of CD44 and HA. A fusion protein containing enhanced green fluorescent protein (EGFP) and a link protein HA-binding motif (EGFP-L) were used to detect cell membrane HA on liver cells and block the adhesion of CD44-positive leukemia with liver cells.

Materials and methods

Cells

The Kasumi-1 human acute myeloid leukemia cell line was kindly provided by Professor S.J. Chen (Shanghai Jiaotong University, Shanghai, China) and maintained in RPMI-1640 medium (Hyclone Laboratories, Inc., Logan, UT, USA) supplemented with 20% fetal bovine serum (FBS) (Life Technologies, Inc., Grand Island, NY, USA) and 1% L-glutamine (Life Technologies, Inc.). The HL-60 human acute myeloid leukemia cell line was purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in RPMI-1640 medium (Hyclone Laboratories, Inc.) supplemented with 10% FBS (Life Technologies, Inc.) and 1% L-glutamine (Life Technologies, Inc.). The L02 human fetal liver cell line and the BEL7404 human hepatoma cell line were purchased from the Shanghai Cell Collection (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Carlsbad, CA, USA), supplemented with 10% FBS (Life Technologies, Inc.) and 1% L-glutamine (Life Technologies, Inc.).

Plasmid construction and recombinant protein preparation

A sequence encoding EGFP was amplified from plasmid pEGFP-C1 using PCR. The sense primer contained an NdeI site (5′-TATCATATGGTGAGCAAGGGCGAG-3′), while the antisense primer contained an XhoI site (5′-TATCTCGAGTT AGCATCTGAGTCC-3′). The EGFP-L fusion gene containing a sequence encoding EGFP and a link protein HA-binding motif with the amino acid sequence 316RYPISRPRKR325(17) was generated by PCR from pEGFP-C1. The sense primer contained an NdeI site (5′-TATCATATGGTGAGCA AGG GCGAG-3′), while the antisense primer contained a sequence encoding the link protein HA-binding motif and an XhoI site (5′-TATCTCGAGTTAGCGCTTTCTGGGTCTGGAGATGGGGTAGCGAGATCTGAGTCCGGACT-3′). PCR products were inserted into the corresponding site of plasmid pET-28α to generate plasmids pET-28α-EGFP and pET-28α-EGFP-L.

The plasmid pET-28α-EGFP or pET-28α-EGFP-L was transformed into Escherichia coli strain M15 and the expression of recombinant proteins was induced by 0.08 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) at room temperature for 6 h. Cells were harvested by centrifugation at 7,104 × g for 15 min followed by resuspension in PBS and disruption by sonication. Bacterial debris was pelleted by centrifugation at 15,984 × g for 15 min. Supernatants were mixed with Ni-NTA slurry (Merck Biosciences, Darmstadt, Germany). The lysate-Ni-NTA mixture was loaded into a column and washed twice with a washing buffer containing 300 mM NaCl, 50 mM sodium phosphate buffer and 20 mM imidazole (pH 8.0). The column was eluted with an elution buffer containing 300 mM NaCl, 50 mM sodium phosphate buffer and 250 mM imidazole (pH 8.0). The eluted protein was dialyzed against PBS at 4°C overnight to remove imidazole. Proteins (∼30 kDa) were then analyzed by SDS-PAGE followed by Coomassie Brilliant Blue staining. The protein concentration was determined using the BCA Protein Assay kit (Thermo Fisher Scientific, Inc., Rockford, IL, USA).

Flow cytometry

For the detection of CD44 expression, Kasumi-1 or HL-60 cells were incubated with fluorescein isothiocyanate (FITC)-conjugated anti-CD44 antibody (BD Biosciences, San Jose, CA, USA) at 4°C for 20 min. FITC-conjugated mouse IgG staining served as the control. Following staining, the cells were analyzed on a BD FACSAria flow cytometer (BD Biosciences).

EGFP-L staining analysis

L02 or BEL7404 cells were cultured in 24-well plates. When cells reached 60–70% confluence, the culture medium was aspirated and 150 μg/ml of EGFP-L was added. Following 20 min of incubation at 4°C, EGFP-L was removed. Cells were then washed with PBS three times. EGFP staining served as the control. A fluorescent microscope (Olympus Corporation, Tokyo, Japan) was used to observe green fluorescence.

Cell-cell adhesion assay

L02 cells were cultured in 24-well plates. When cells reached 60–70% confluence, the culture medium was aspirated, and Kasumi-1 or HL-60 cells were added. After 30–45 min, any Kasumi-1 or HL-60 cells that were non-adherent to L02 cells were removed. A microscope (Olympus Corporation) was used to observe cell-cell adhesion.

For the HA blocking experiments, Kasumi-1 cells were incubated with 1 mg/ml of HA at 4°C for 10 min. Cells were then added to the same volume of culture medium followed by coculture with L02 precultured in 24-well plates. After 30 min, Kasumi-1 cells that were non-adherent to L02 cells were collected and counted. PBS treatment served as the control.

For the EGFP-L blocking experiments, L02 cells cultured in 24-well plates were incubated with 150 μg/ml of EGFP-L or EGFP at 4°C for 30 min. Kasumi-1 cells were then added. After 45 min, Kasumi-1 cells that were non-adherent to L02 cells were collected and counted.

Statistical analysis

Differences among the treatment groups were assessed by ANOVA. P<0.05 was considered to indicate a statistically significant difference.

Results

Leukemic cells adhere to liver cells through CD44

The Kasumi-1 leukemia cell line adhered to L02 liver cells. However, this cell-cell adhesion did not occur in HL-60 cells (Fig. 1A). By analyzing the expression of cell membrane proteins, we determined that the majority of the Kasumi-1 cells expressed CD44, and most of the HL-60 cells had a CD44-negative phenotype (Fig. 1B). Therefore, we hypothesized that leukemic cells may adhere to L02 cells through CD44. To examine this hypothesis, HA, a natural ligand for CD44 was used to pretreat Kasumi-1 cells followed by the coculture of Kasumi-1 with L02 cells. As shown in Fig. 2, the treatment of Kasumi-1 cells with HA significantly blocked the adhesion of Kasumi-1 with L02 cells (37.6±4.8% of adherent Kasumi-1 cells), compared to the PBS control (72.3±1.4% of adherent Kasumi-1 cells). These results demonstrated that CD44 is involved in the adhesion of leukemic cells with liver cells.

Figure 1 Adhesion of CD44-positive Kasumi-1 cells, but not CD44-negative HL-60 cells with liver cells. (A) Adherence of Kasumi-1 or HL-60 cells with L02 liver cells. Kasumi-1 or HL-60 cells were cocultured with L02 cells and then washed with PBS. Adherent cells were observed using a microscope (magnification, ×200). Data are representative of three independent experiments. (B) Flow cytometric analysis of the CD44 expression of Kasumi-1 and HL-60 cells. SSC, subsurface cisternae; CD44-FITC, CD44-fluorescein isothiocyanate.

Figure 2 Adhesion of Kasumi-1 cells with liver cells was blocked by hyaluronan (HA). Kasumi-1 cells were pretreated with HA followed by coculture with L02 cells. Phosphate-buffered saline (PBS) pretreatment served as the control. Non-adherent Kasumi-1 cells were collected and counted. The percentage of adherent Kasumi-1 cells was calculated. Values are presented as the mean ± standard error of the mean (SEM) from three separate experiments. *P<0.05 compared to the control.

Liver cells express HA on the cell membrane

HA is a linear glycosaminoglycan with the molecular mass of a few million Daltons and can be secreted by cells to the ECM or associated with the plasma membrane to form a pericellular coat (18). Certain cell-associated and ECM proteins, such as CD44, link protein and collagen VI (6–10) have been found to mediate the biological functions of HA. To determine the liver cell membrane component involved in the adhesion with CD44-positive leukemia cells, EGFP-L, a fusion protein containing EGFP and a link protein HA-binding motif were generated to detect the cell membrane HA on liver cells. Recombinant EGFP was used as the control. The majority of L02 and BEL7404 cells were labeled with EGFP-L as compared to the EGFP control, indicating that liver cells L02 and BEL7404 expressed HA on the cell membrane (Fig. 3). Notably, EGFP-L formed bright dots on the cell membrane of L02 and BEL7404 cells, suggesting that HA may associate with certain special membrane structures in liver cells. Taken together, these results demonstrated that the CD44-HA interaction was involved in the adhesion of leukemic cells with liver cells.

Figure 3 Detection of the liver cell hyaluronan (HA), using enhanced green fluorescent protein and a link protein HA-binding motif (EGFP-L) staining. L02 or BEL7404 liver cells were cultured followed by EGFP-L staining. EGFP served as the control. Green fluorescence was observed using a fluorescence microscope (magnification, ×200).

EGFP-L abrogates the adhesion of leukemic and liver cells

To determine the effect of EGFP-L on the adhesion of Kasumi-1 with L02 cells, L02 cells were pretreated with EGFP-L and then cocultured with Kasumi-1 cells. Recombinant EGFP was used as the control. As shown in Fig. 4, treatment of L02 cells with EGFP-L significantly blocked the adhesion of L02 cells with Kasumi-1 cells (36.8±2.5% of adherent Kasumi-1 cells), compared to EGFP treatment (49.6±3.0% of adherent Kasumi-1 cells). These results indicate that the link protein HA-binding motif is effective in inhibiting the adhesion between CD44-positive leukemia cells with HA-positive liver cells.

Figure 4 Adhesion of Kasumi-1 cells with L02 cells was blocked by enhanced green fluorescent protein and a link protein hyaluronan (HA)-binding motif (EGFP-L). L02 cells were cultured and pretreated with EGFP-L followed by coculture with Kasumi-1 cells. EGFP pretreatment was used as the control. Non-adherent Kasumi-1 cells were collected and counted. The percentage of adherent Kasumi-1 cells was calculated. Values are presented as the mean ± standard error of the mean (SEM) from four separate experiments. *P<0.05.

Discussion

Ligation of CD44 with HA is associated with the drug resistance of cancer cells. Furthermore, CD44-HA interaction activates ErbB2 and PI3K to stimulate multidrug transporter MDR1, resulting in the drug resistance of breast and ovarian cancer cells (13,19). CD44 and MDR1 have been found to be coexpressed in small cell lung cancer chemoresistant cells (20). Drug resistance induced by the CD44-HA interaction has also been observed in head and neck cancer (15,16). Notably, the ligation of CD44 with monoclonal antibodies has been demonstrated to decrease drug-induced apoptosis in acute myeloid leukemia cells (21). The results of this study have shown that the link protein HA-binding motif bound with HA in liver cell membranes and abrogated the adhesion of CD44-positive leukemia cells with liver cells. These findings may provide insight into developing novel methods to inhibit liver metastasis in leukemia.

Acknowledgements

This study was supported by the National Natural Science Foundation of China (30801379).

References