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Article

Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients

  • Authors:
    • Hideaki Yashima
    • Kimihiro Shimizu
    • Takuya Araki
    • Tohru Aomori
    • Yoichi Ohtaki
    • Toshiteru Nagashima
    • Yasuaki Enokida
    • Jun Atsumi
    • Tomonori Nakamura
    • Izumi Takeyoshi
    • Koujirou Yamamoto
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan, Department of Thoracic and Visceral Organ Surgery, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan
  • Pages: 714-718
    |
    Published online on: June 3, 2014
       https://doi.org/10.3892/mco.2014.302
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Abstract

Molecular‑targeted therapy has not been established in non-adenocarcinoma lung cancer (non-AdLC), as no targets that affect the clinical efficacy of molecular‑targeted drugs have yet been identified. In this study, we investigated the frequency of genetic variations in discoidin domain receptor 2 (DDR2), v-raf murine sarcoma viral oncogene homolog B1 (BRAF), epidermal growth factor receptor (EGFR) and v-Ki‑ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) in non‑AdLC patients, in order to evaluate the possibility of genetic mutations in these genes being used as therapeutic targets for the treatment of patients with non‑AdLC. For this purpose, we enrolled 150 non-AdLC patients who had undergone surgery at the Gunma University Hospital between December, 2003 and December, 2012. Genetic mutations in the EGFR, KRAS, DDR2 and BRAF genes were detected by a sequencing method or probe assay using DNA derived from cancer tissues. No somatic mutations in DDR2 or BRAF were detected in non-AdLC patients. Conversely, genetic mutations in EGFR exon 19 were found in 3 squamous cell carcinoma (SCC) and 3 adenosquamous carcinoma patients, whereas KRAS codon 12 mutations were also found in 3 SCC patients and 1 large‑cell neuroendocrine carcinoma patient. EGFR and KRAS mutations were mutually exclusive. This study indicated that, although DDR2 and BRAF mutations may only rarely be used as therapeutic targets, EGFR and KRAS mutations may represent candidate therapeutic targets, at least in the non-AdLC patients investigated.
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Copy and paste a formatted citation
Spandidos Publications style
Yashima H, Shimizu K, Araki T, Aomori T, Ohtaki Y, Nagashima T, Enokida Y, Atsumi J, Nakamura T, Takeyoshi I, Takeyoshi I, et al: Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients. Mol Clin Oncol 2: 714-718, 2014.
APA
Yashima, H., Shimizu, K., Araki, T., Aomori, T., Ohtaki, Y., Nagashima, T. ... Yamamoto, K. (2014). Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients. Molecular and Clinical Oncology, 2, 714-718. https://doi.org/10.3892/mco.2014.302
MLA
Yashima, H., Shimizu, K., Araki, T., Aomori, T., Ohtaki, Y., Nagashima, T., Enokida, Y., Atsumi, J., Nakamura, T., Takeyoshi, I., Yamamoto, K."Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients". Molecular and Clinical Oncology 2.5 (2014): 714-718.
Chicago
Yashima, H., Shimizu, K., Araki, T., Aomori, T., Ohtaki, Y., Nagashima, T., Enokida, Y., Atsumi, J., Nakamura, T., Takeyoshi, I., Yamamoto, K."Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients". Molecular and Clinical Oncology 2, no. 5 (2014): 714-718. https://doi.org/10.3892/mco.2014.302
Copy and paste a formatted citation
x
Spandidos Publications style
Yashima H, Shimizu K, Araki T, Aomori T, Ohtaki Y, Nagashima T, Enokida Y, Atsumi J, Nakamura T, Takeyoshi I, Takeyoshi I, et al: Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients. Mol Clin Oncol 2: 714-718, 2014.
APA
Yashima, H., Shimizu, K., Araki, T., Aomori, T., Ohtaki, Y., Nagashima, T. ... Yamamoto, K. (2014). Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients. Molecular and Clinical Oncology, 2, 714-718. https://doi.org/10.3892/mco.2014.302
MLA
Yashima, H., Shimizu, K., Araki, T., Aomori, T., Ohtaki, Y., Nagashima, T., Enokida, Y., Atsumi, J., Nakamura, T., Takeyoshi, I., Yamamoto, K."Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients". Molecular and Clinical Oncology 2.5 (2014): 714-718.
Chicago
Yashima, H., Shimizu, K., Araki, T., Aomori, T., Ohtaki, Y., Nagashima, T., Enokida, Y., Atsumi, J., Nakamura, T., Takeyoshi, I., Yamamoto, K."Assessment of DDR2, BRAF, EGFR and KRAS mutations as therapeutic targets in non-adenocarcinoma lung cancer patients". Molecular and Clinical Oncology 2, no. 5 (2014): 714-718. https://doi.org/10.3892/mco.2014.302
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