CD4+ T‑lymphocytes are activated by surgical stress following colorectal resection in cancer patients

Maki,Kenji; Takeno,Shinsuke; Aisu,Naoya; Yamashita,Kanefumi; Naito,Masayasu; Hoshino,Seiichiro; Yamashita,Yuichi

doi:10.3892/mco.2014.442

January-February 2015 Volume 3 Issue 1

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January-February 2015 Volume 3 Issue 1

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CD4+ T‑lymphocytes are activated by surgical stress following colorectal resection in cancer patients

Authors:
- Kenji Maki
- Shinsuke Takeno
- Naoya Aisu
- Kanefumi Yamashita
- Masayasu Naito
- Seiichiro Hoshino
- Yuichi Yamashita
View Affiliations / Copyright

Affiliations: Department of Gastroenterological Surgery, Faculty of Medicine, Fukuoka University, Fukuoka, Fukuoka 814‑0180, Japan
Pages: 79-82
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Published online on: October 13, 2014

https://doi.org/10.3892/mco.2014.442
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Abstract

The aim of the present study was to measure adenosine triphosphate (ATP) levels in CD4+ T cells as a marker of T‑cell activity following surgery for colorectal cancer using the ImmuKnow assay kit. A total of 16 consecutive patients who underwent surgical resection for colorectal cancer between August and December, 2012 were enrolled in this study, of whom 7 underwent laparoscopic resection and 9 underwent open abdominal surgery. The intracellular ATP levels in CD4+ T‑lymphocytes were measured using the ImmuKnow assay kit preoperatively and on the 1st, 4th and 8th postoperative days, as were the white blood cell (WBC) count, lymphocyte count and C‑reactive protein (CRP) levels. The ATP level of the CD4+ T‑cells was significantly elevated on the 1st day following surgery compared to the preoperative level (P<0.01) and gradually returned to preoperative levels; the lymphocyte count was significantly decreased on the 1st postoperative day (P<0.001). In addition, the ImmuKnow assay demonstrated that only the ATP level, but not the WBC count, lymphocyte count or CRP level, exhibited a significant difference on the 1st (P=0.080) and 8th (P=0.042) postoperative days between the laparoscopic and open abdominal surgery groups. In conclusion, the ATP level of CD4+ T‑lymphocytes was increased in response to surgical stress, in tandem with a decrease in the lymphocyte count. Therefore, the ImmuKnow assay kit may be clinically applicable for monitoring the immune response following surgery, as it exhibits a higher sensitivity compared to other assays.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

CD4+ T‑lymphocytes are activated by surgical stress following colorectal resection in cancer patients

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