In vivo detection of desmoplastic reaction using endocytoscopy: A new diagnostic marker of submucosal or more extensive invasion in colorectal carcinoma

Sugihara,Yuusaku; Kudo,Shin‑Ei; Miyachi,Hideyuki; Wakamura,Kunihiko; Mori,Yuichi; Misawa,Masashi; Hisayuki,Tomokazu; Kudo,Toyoki; Hayashi,Takemasa; Hamatani,Shigeharu; Okoshi,Shogo; Okada,Hiroyuki

doi:10.3892/mco.2017.1138

March-2017 Volume 6 Issue 3

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March-2017 Volume 6 Issue 3

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Article Open Access

In vivo detection of desmoplastic reaction using endocytoscopy: A new diagnostic marker of submucosal or more extensive invasion in colorectal carcinoma

Authors:
- Yuusaku Sugihara
- Shin‑Ei Kudo
- Hideyuki Miyachi
- Kunihiko Wakamura
- Yuichi Mori
- Masashi Misawa
- Tomokazu Hisayuki
- Toyoki Kudo
- Takemasa Hayashi
- Shigeharu Hamatani
- Shogo Okoshi
- Hiroyuki Okada
View Affiliations / Copyright

Affiliations: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Kanagawa 224‑0032, Japan, Department of Pathology, The Jikei University School of Medicine, Tokyo 105‑8461, Japan, Department of Internal Medicine, School of Life Dentistry at Niigata, The Nippon Dental University, Niigata 951‑1500, Japan, Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700‑8558, Japan

Copyright: © Sugihara et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 291-295
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Published online on: January 23, 2017

https://doi.org/10.3892/mco.2017.1138
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Abstract

The pathological determination of desmoplastic reaction (DR) in colorectal carcinoma is useful for predicting extensive submucosal invasion. The aim of the present study was to determine the usefulness of endocytoscopy (EC) in detecting DR. A total of 72 cases of colorectal cancer with submucosal invasion (EC classification, EC3b) were evaluated. The utility of fine granular structure (FGS) observed via EC for the prediction of the presence of DR in the most superficial tumor layers was assessed. Of the 72 lesions, 26 were positive for FGS, and the majority of these lesions (23/26, 88.5%) exhibited a DR, indicating a significant association. The overall accuracy of the identification of FGS via EC that was predictive of a DR was 87.3%. The presence of FGS detected by EC was significantly associated with the presence of a DR, suggesting the clinical usefulness of EC in planning treatment for colon cancer with submucosal invasion.

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1	Kudo S, Hirota S, Nakajima T, Hosobe S, Kusaka H, Kobayashi T, Himori M and Yagyuu A: Colorectal tumors and pit pattern. J Clin Pathol. 47:880–885. 1994. View Article : Google Scholar : PubMed/NCBI
2	Kudo S, Rubio CA, Teixeira CR, Kashida H and Kogure E: Pit pattern in colorectal neoplasia: Endoscopic magnifying view. Endoscopy. 33:367–373. 2001. View Article : Google Scholar : PubMed/NCBI
3	Inoue H, Kazawa T, Sato Y, Satodate H, Sasajima K, Kudo SE and Shiokawa A: In vivo observation of living cancer cells in the esophagus, stomach, and colon using catheter-type contact endoscope, ‘Endo-Cytoscopy system’. Gastrointest Endosc Clin N Am. 14:589–594, x-xi. 2004. View Article : Google Scholar : PubMed/NCBI
4	Sasajima K, Kudo SE, Inoue H, Takeuchi T, Kashida H, Hidaka E, Kawachi H, Sakashita M, Tanaka J and Shiokawa A: Real-time in vivo virtual histology of colorectal lesions when using the endocytoscopy system. Gastrointest Endosc. 63:1010–1017. 2006. View Article : Google Scholar : PubMed/NCBI
5	Kudo SE, Misawa M, Wada Y, Nakamura H, Kataoka S, Maeda Y, Toyoshima N, Hayashi S, Kutsukawa M, Oikawa H, et al: Endocytoscopic microvasculature evaluation is a reliable new diagnostic method for colorectal lesions (with video). Gastrointest Endosc. 82:912–923. 2015. View Article : Google Scholar : PubMed/NCBI
6	Mori Y, Kudo S, Ikehara N, Wakamura K, Wada Y, Kutsukawa M, Misawa M, Kudo T, Kobayashi Y, Miyachi H, et al: Comprehensive diagnostic ability of endocytoscopy compared with biopsy for colorectal neoplasms: A prospective randomized noninferiority trial. Endoscopy. 45:98–105. 2013. View Article : Google Scholar : PubMed/NCBI
7	Kudo SE, Wakamura K, Ikehara N, Mori Y, Inoue H and Hamatani S: Diagnosis of colorectal lesions with a novel endocytoscopic classification-a pilot study. Endoscopy. 43:869–875. 2011. View Article : Google Scholar : PubMed/NCBI
8	Martin M, Pujuguet P and Martin F: Role of stromal myofibroblasts infiltrating colon cancer in tumor invasion. Pathol Res Pract. 192:712–717. 1996. View Article : Google Scholar : PubMed/NCBI
9	Hirose M, Fukui H, Igarashi Y, Fujimori Y, Katake Y, Sekikawa A, Ichikawa K, Tomita S, Imura J, Ajioka Y, et al: Detection of desmoplastic reaction in biopsy specimens is useful for predicting the depth of invasion of early colorectal cancer: A Japanese collaborative study. J Gastroenterol. 45:1212–1218. 2010. View Article : Google Scholar : PubMed/NCBI
10	Okamoto Y, Fujimori T, Ohkura Y, Sugai T, Arai T, Watanabe G, Wada R, Ueno H, Togashi K, Yao T, et al: Histological assessment of intra- and inter-institutional reliabilities in detection of desmoplastic reaction in biopsy specimens of early colorectal carcinomas. Pathol Int. 63:539–545. 2013. View Article : Google Scholar : PubMed/NCBI
11	Kunz LA and Knuechel R: Tumor-associated fibroblasts (part I): Active stromal participants in tumor development and progression? Histol Histopathol. 17:599–621. 2002.PubMed/NCBI
12	Angeli F, Koumakis G, Chen MC, Kumar S and Delinassios JG: Role of stromal fibroblasts in cancer: Promoting or impeding? Tumour Biol. 30:109–120. 2009. View Article : Google Scholar : PubMed/NCBI
13	Kudo SE, Sugihara Y, Kida H, Ishida F, Miyachi H, Mori Y, Misawa M, Hisayuki T, Kodama K, Wakamura K, et al: Depressed-type colonic lesions and ‘de novo’ cancer in familial adenomatous polyposis: A colonoscopist's viewpoint. ISRN Gastroenterol. 2013:8381342013. View Article : Google Scholar : PubMed/NCBI
14	Kimura R, Fujimori T, Ichikawa K, Ajioka Y, Ueno H, Ohkura Y, Kashida H, Togashi K, Yao T, Wada R, et al: Desmoplastic reaction in biopsy specimens of early colorectal cancer: A Japanese prospective multicenter study. Pathol Int. 62:525–531. 2012. View Article : Google Scholar : PubMed/NCBI
15	Landis JR and Koch GG: The measurement of observer agreement for categorical data. Biometrics. 33:159–174. 1977. View Article : Google Scholar : PubMed/NCBI
16	Worthley DL, Giraud AS and Wang TC: Stromal fibroblasts in digestive cancer. Cancer Microenviron. 3:117–125. 2010. View Article : Google Scholar : PubMed/NCBI