Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids

Sievers,Denise; Bunzendahl,Jens; Frosch,Alice; Perske,Christina; Hemmerlein,Bernhard; Schliephake,Henning; Brockmeyer,Phillipp

doi:10.3892/mco.2017.1514

February-2018 Volume 8 Issue 2

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February-2018 Volume 8 Issue 2

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Article

Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids

Authors:
- Denise Sievers
- Jens Bunzendahl
- Alice Frosch
- Christina Perske
- Bernhard Hemmerlein
- Henning Schliephake
- Phillipp Brockmeyer
View Affiliations / Copyright

Affiliations: Department of Oral and Maxillofacial Surgery, University Medical Centre Goettingen, D‑37075 Goettingen, Germany, Department of Pathology, University Medical Centre Goettingen, D‑37075 Goettingen, Germany
Pages: 323-325
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Published online on: November 27, 2017

https://doi.org/10.3892/mco.2017.1514
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Abstract

Three‑dimensional (3D) multicellular spheroids (MCS) are considered suitable models in cancer research and anticancer drug development. Although studying the complex tumour characteristics from all different degrees of malignancy is vital, MCS generation has only been described in a few moderately‑ and poorly differentiated oral squamous cell carcinoma (OSCC) cell lines. No previous study has demonstrated the MCS formation in a highly differentiated OSCC cell line. For the first time, the present study aimed to generate MCS from the highly differentiated OSCC cell line BHY. BHY spheroids were grown in three independent experiments in 96‑well plates through the use of the liquid overlay technique. Although BHY cells are grow slowly and are difficult to culture, they formed compact MCS within 24 h. After 3 days of incubation, no further increase in spheroid size was observed. MCS were harvested, paraffin‑embedded and 2 µm tissue sections were prepared for further analysis. The diameter and volume of each spheroid were determined. BHY MCS diameter ranged between 46.76 and 233.26 µm, with a volume range from 5.35x104‑6.65x106 µm³. In conclusion, using the liquid overlay technique, the highly differentiated OSCC cell line BHY forms different sized spheroids, which may be used for further investigations.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids

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Abstract

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