Open Access

Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9‑mediated knockout of CDK2 in A375 melanocytes

  • Authors:
    • Houguang Liu
    • Zheng Li
    • Shanshan Huo
    • Qiongling Wei
    • Ling Ge
  • View Affiliations

  • Published online on: November 20, 2019     https://doi.org/10.3892/mco.2019.1952
  • Pages: 9-14
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cutaneous melanoma is one of the most common malignant skin tumors, with a continuously increasing incidence. Cyclin‑dependent kinase (CDK) 2 is a key regulator of G1‑S transition and modulation of G2 progression; however, its role in cancer is a matter of debate. In the present study, a lentivirus expressing single‑guide RNA (sgRNA) was constructed to knock out CDK2 using CRISP/Cas9 technology, in order to confirm the role of CDK2 in A375 human melanoma cells. The results demonstrated that CDK2 knockout induced G0/G1 phase arrest and early apoptosis by downregulating the expression of CDK4 and cyclin A2, and by upregulating the expression of cyclin D1. These results suggest that therapeutic strategies designed to target CDK2 using CRISP/Cas9 may improve the treatment outcome of cutaneous melanoma.
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January-2020
Volume 12 Issue 1

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Liu H, Li Z, Huo S, Wei Q and Ge L: Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9‑mediated knockout of CDK2 in A375 melanocytes. Mol Clin Oncol 12: 9-14, 2020
APA
Liu, H., Li, Z., Huo, S., Wei, Q., & Ge, L. (2020). Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9‑mediated knockout of CDK2 in A375 melanocytes. Molecular and Clinical Oncology, 12, 9-14. https://doi.org/10.3892/mco.2019.1952
MLA
Liu, H., Li, Z., Huo, S., Wei, Q., Ge, L."Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9‑mediated knockout of CDK2 in A375 melanocytes". Molecular and Clinical Oncology 12.1 (2020): 9-14.
Chicago
Liu, H., Li, Z., Huo, S., Wei, Q., Ge, L."Induction of G0/G1 phase arrest and apoptosis by CRISPR/Cas9‑mediated knockout of CDK2 in A375 melanocytes". Molecular and Clinical Oncology 12, no. 1 (2020): 9-14. https://doi.org/10.3892/mco.2019.1952