HBV integrated genomic characterization revealed hepatocyte genomic alterations in HBV‑related hepatocellular carcinomas

Yang,Ming; Yang,Guiqi; Li,Fengyan; Ou,Minglin; Li,Chunhong; Chen,Jiejing; Lin,Hua; Zhang,Yue; Xue,Wen; Wu,Yan; Xu,Yong; Sui,Weiguo; Dai,Yong

doi:10.3892/mco.2020.2149

HBV integrated genomic characterization revealed hepatocyte genomic alterations in HBV‑related hepatocellular carcinomas

Authors:
- Ming Yang
- Guiqi Yang
- Fengyan Li
- Minglin Ou
- Chunhong Li
- Jiejing Chen
- Hua Lin
- Yue Zhang
- Wen Xue
- Yan Wu
- Yong Xu
- Weiguo Sui
- Yong Dai
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Affiliations: Guangxi Key Laboratory of Metabolic Disease Research, Central Laboratory, Nephrology Department of Guilin No. 924 Hospital, Guilin, Guangxi 541002, P.R. China, Clinical Medical Research Center of The Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China
Published online on: October 1, 2020 https://doi.org/10.3892/mco.2020.2149
Article Number: 79
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is one of the most lethal malignancies that is closely associated with the Hepatitis B virus (HBV). HBV integration into host genomes can induce instability and the aberrant expression of human genomic DNA. To directly assess HBV integration breakpoints at whole genome level, four small sequencing libraries were constructed and the HBV integration profiles of four patients with HCC were characterized. In total, the current study identified 11,800,974, 11,216,998, 11,026,546 and 11,607,842 clean reads for patients 1‑3 and 4, respectively, of which 92.82, 95.95, 97.21 and 97.29% were properly aligned to the hybrid reference genome. In addition, 220 HBV integration events were detected from the tumor tissues of four patients with HCC and an average of 55 breakpoints per sample was calculated. The results indicated that HBV integration events may be implicated in HCC physiologies and diseases. The results acquired may also provide insight into the pathogenesis of HCC, which may be valuable for future HCC therapy.

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