An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model
- Yuki Hanamatsu
- Chiemi Saigo
- Yusuke Kito
- Tamotsu Takeuchi
Affiliations: Department of Pathology and Translational Research, Gifu University Graduate School of Medicine, Gifu 501‑1193, Japan
- Published online on: November 12, 2020 https://doi.org/10.3892/mco.2020.2171
Copyright: © Hanamatsu
et al. This is an open access article distributed under the
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Clear cell sarcoma (CCS) affects the deep soft tissues in young adults and is known to have high rates of metastasis, including lymphatic metastasis. In our previous study an xenoplant model of CCS was established, which exhibited local tumor growth, lymphatic metastasis, and distant metastasis in SCID‑Beige mice. In the current study, the role of NK cells during metastasis in the same xenoplant murine model was investigated. Injection of murine or human NK cells significantly suppressed the metastasis of HS‑MM CCS cells in SCID‑Beige mice. Notably, reverse transcription‑quantitative PCR analysis demonstrated that injection of NK cells did not alter the mRNA expression levels of ERSR1‑ATF1, which is specifically transcribed in CCS, in the buffy coat of circulating blood cells of HS‑MM‑xenoplanted SCID‑Beige mice. BALB/c nude mice xenoplanted with HS‑MM cells exhibited local growth without evident metastasis, whereas inoculation with the anti‑asialo‑GM1 antibody, which has previously been found to abolish NK‑cell activity, resulted in metastasis of HS‑MM cells in BALB/c nude mice. The injection of the anti‑CD96 antibody, which increases the cytotoxicity of NK cells, significantly suppressed the metastasis of HS‑MM cells in SCID‑Beige mice. These results indicated that NK cells impaired the metastatic tumor microenvironments in the present mice xenoplant model.