Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular and Clinical Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9450 Online ISSN: 2049-9469
Journal Cover
January-2021 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2021 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model

  • Authors:
    • Yuki Hanamatsu
    • Chiemi Saigo
    • Yusuke Kito
    • Tamotsu Takeuchi
  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Translational Research, Gifu University Graduate School of Medicine, Gifu 501‑1193, Japan
    Copyright: © Hanamatsu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 9
    |
    Published online on: November 12, 2020
       https://doi.org/10.3892/mco.2020.2171
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Clear cell sarcoma (CCS) affects the deep soft tissues in young adults and is known to have high rates of metastasis, including lymphatic metastasis. In our previous study an xenoplant model of CCS was established, which exhibited local tumor growth, lymphatic metastasis, and distant metastasis in SCID‑Beige mice. In the current study, the role of NK cells during metastasis in the same xenoplant murine model was investigated. Injection of murine or human NK cells significantly suppressed the metastasis of HS‑MM CCS cells in SCID‑Beige mice. Notably, reverse transcription‑quantitative PCR analysis demonstrated that injection of NK cells did not alter the mRNA expression levels of ERSR1‑ATF1, which is specifically transcribed in CCS, in the buffy coat of circulating blood cells of HS‑MM‑xenoplanted SCID‑Beige mice. BALB/c nude mice xenoplanted with HS‑MM cells exhibited local growth without evident metastasis, whereas inoculation with the anti‑asialo‑GM1 antibody, which has previously been found to abolish NK‑cell activity, resulted in metastasis of HS‑MM cells in BALB/c nude mice. The injection of the anti‑CD96 antibody, which increases the cytotoxicity of NK cells, significantly suppressed the metastasis of HS‑MM cells in SCID‑Beige mice. These results indicated that NK cells impaired the metastatic tumor microenvironments in the present mice xenoplant model.
View Figures

Figure 1

Figure 2

View References

1 

Zucman J, Delattre O, Desmaze C, Epstein AL, Stenman G, Speleman F, Fletchers CD, Aurias A and Thomas G: EWS and ATF-1 gene fusion induced by t(12;22) translocation in malignant melanoma of soft parts. Nat Genet. 4:341–345. 1993.PubMed/NCBI View Article : Google Scholar

2 

Thway K and Fisher C: Tumors with EWSR1-CREB1 and EWSR1-ATF1 fusions: The current status. Am J Surg Pathol. 36:e1–e11. 2012.PubMed/NCBI View Article : Google Scholar

3 

Enzinger FM: Clear-cell sarcoma of tendons and aponeuroses. An analysis of 21 cases. Cancer. 18:1163–1174. 1965.PubMed/NCBI View Article : Google Scholar

4 

Chung EB and Enzinger FM: Malignant melanoma of soft parts. A reassessment of clear cell sarcoma. Am J Surg Pathol. 7:405–413. 1983.PubMed/NCBI View Article : Google Scholar

5 

Ibrahim RM, Steenstrup Jensen S and Juel J: Clear cell sarcoma-A review. J Orthop. 15:963–966. 2018.PubMed/NCBI View Article : Google Scholar

6 

Andreou D and Tunn PU: Sentinel node biopsy in soft tissue sarcoma. Recent Results Cancer Res. 179:25–36. 2009.PubMed/NCBI View Article : Google Scholar

7 

Andreou D, Boldt H, Werner M, Hamann C, Pink D and Tunn PU: Sentinel node biopsy in soft tissue sarcoma subtypes with a high propensity for regional lymphatic spread-results of a large prospective trial. Ann Oncol. 24:1400–1405. 2013.PubMed/NCBI View Article : Google Scholar

8 

Mavrogenis A, Bianchi G, Stavropoulos N, Papagelopoulos P and Ruggieri P: Clinicopathological features, diagnosis and treatment of clear cell sarcoma/melanoma of soft parts. Hippokratia. 17:298–302. 2013.PubMed/NCBI

9 

Egawa Y, Saigo C, Kito Y, Moriki T and Takeuchi T: Therapeutic potential of CPI-613 for targeting tumorous mitochondrial energy metabolism and inhibiting autophagy in clear cell sarcoma. PLoS One. 13(e0198940)2018.PubMed/NCBI View Article : Google Scholar

10 

Kawashima K, Saigo C, Kito Y, Hanamatsu Y, Egawa Y and Takeuchi T: CD151 confers metastatic potential to clear cell sarcoma of the soft tissue in animal model. Oncol Lett. 17:4811–4818. 2019.PubMed/NCBI View Article : Google Scholar

11 

MacDougall JR, Croy BA, Chapeau C and Clark DA: Demonstration of a splenic cytotoxic effector cell in mice of genotype SCID/SCID.BG/BG. Cell Immunol. 130:106–117. 1990.PubMed/NCBI View Article : Google Scholar

12 

Budzynski W and Radzikowski C: Cytotoxic cells in immunodeficient athymic mice. Immunopharmacol Immunotoxicol. 16:319–346. 1994.PubMed/NCBI View Article : Google Scholar

13 

Sonobe H, Furihata M, Iwata J, Ohtsuki Y, Mizobuchi H, Yamamoto H and Kumano O: Establishment and characterization of a new human clear-cell sarcoma cell-line, HS-MM. J Pathol. 169:317–322. 1993.PubMed/NCBI View Article : Google Scholar

14 

Sonobe H, Takeuchi T, Taguchi T, Shimizu K, Iwata J, Furihata M and Ohtsuki Y: Further characterization of the human clear cell sarcoma cell line HS-MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWSR1/ATF-1 transcript. J Pahol. 187:594–597. 1999.PubMed/NCBI View Article : Google Scholar

15 

Takeuchi T, Adachi Y and Nagayama T: A WWOX-binding molecule, transmembrane protein 207, is related to the invasiveness of gastric signet-ring cell carcinoma. Carcinogenesis. 33:548–554. 2012.PubMed/NCBI View Article : Google Scholar

16 

Asano Y, Takeuchi T, Okubo H, Saigo C, Kito Y, Iwata Y, Futamura M and Yoshida K: Nuclear localization of LDL receptor-related protein 1B in mammary gland carcinogenesis. J Mol Med (Berl). 97:257–268. 2019.PubMed/NCBI View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

18 

Kanda Y: Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone Marrow Transplant. 48:452–458. 2013.PubMed/NCBI View Article : Google Scholar

19 

R Core Team: R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, 2014.

20 

Chan CJ, Martinet L, Gilfillan S, Souza-Fonseca-Guimaraes F, Chow MT, Town L, Ritchie DS, Colonna M, Andrews DM and Smyth MJ: The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions. Nat Immunol. 15:431–438. 2014.PubMed/NCBI View Article : Google Scholar

21 

Ochoa MC, Minute L, Rodriguez I, Garasa S, Perez-Ruiz E, Inogés S, Melero I and Berraondo P: Antibody-dependent cell cytotoxicity: Immunotherapy strategies enhancing effector NK cells. Immunol Cell Biol. 95:347–355. 2017.PubMed/NCBI View Article : Google Scholar

22 

Faltas B: Cornering metastases: Therapeutic targeting of circulating tumor cells and stem cells. Front Oncol. 2(68)2012.PubMed/NCBI View Article : Google Scholar

23 

Narasimhan PB, Eggert T, Zhu YP, Marcovecchio P, Meyer MA, Wu R and Hedrick CC: Patrolling monocytes control NK cell expression of activating and stimulatory receptors to curtail lung metastases. J Immunol. 204:192–198. 2020.PubMed/NCBI View Article : Google Scholar

24 

Bi J and Tian Z: NK cell dysfunction and checkpoint immunotherapy. Front Immunol. 10(1999)2019.PubMed/NCBI View Article : Google Scholar

25 

López-Soto A, Gonzalez S, Smyth MJ and Galluzzi L: Control of metastasis by NK cells. Cancer Cell. 32:135–154. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hanamatsu Y, Saigo C, Kito Y and Takeuchi T: An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model. Mol Clin Oncol 14: 9, 2021.
APA
Hanamatsu, Y., Saigo, C., Kito, Y., & Takeuchi, T. (2021). An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model. Molecular and Clinical Oncology, 14, 9. https://doi.org/10.3892/mco.2020.2171
MLA
Hanamatsu, Y., Saigo, C., Kito, Y., Takeuchi, T."An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model". Molecular and Clinical Oncology 14.1 (2021): 9.
Chicago
Hanamatsu, Y., Saigo, C., Kito, Y., Takeuchi, T."An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model". Molecular and Clinical Oncology 14, no. 1 (2021): 9. https://doi.org/10.3892/mco.2020.2171
Copy and paste a formatted citation
x
Spandidos Publications style
Hanamatsu Y, Saigo C, Kito Y and Takeuchi T: An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model. Mol Clin Oncol 14: 9, 2021.
APA
Hanamatsu, Y., Saigo, C., Kito, Y., & Takeuchi, T. (2021). An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model. Molecular and Clinical Oncology, 14, 9. https://doi.org/10.3892/mco.2020.2171
MLA
Hanamatsu, Y., Saigo, C., Kito, Y., Takeuchi, T."An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model". Molecular and Clinical Oncology 14.1 (2021): 9.
Chicago
Hanamatsu, Y., Saigo, C., Kito, Y., Takeuchi, T."An obstructive role of NK cells on metastatic growth of clear‑cell sarcoma cells in a xenoplant murine model". Molecular and Clinical Oncology 14, no. 1 (2021): 9. https://doi.org/10.3892/mco.2020.2171
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team