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Klotho promoter methylation status and its prognostic value in ovarian cancer

  • Authors:
    • Maryam H. Al‑Zahrani
    • Fatimah M. Yahya
    • Mourad Assidi
    • Ashraf Dallol
    • Abdelbaset Buhmeida
  • View Affiliations / Copyright

    Affiliations: Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia, Center of Excellence in Genomic Medicine Research, King Abdulaziz University, Jeddah 21589, Saudi Arabia
    Copyright: © Al‑Zahrani et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 181
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    Published online on: July 3, 2021
       https://doi.org/10.3892/mco.2021.2343
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Abstract

Among all gynecological cancers, ovarian cancer (OC) is one of the deadliest types of cancer worldwide. Epigenetic silencing of some genes has been reported to be associated with OC. In this context, Klotho (KL) gene methylation is a promising biomarker for OC. The present study aimed to investigate the methylation profiles of KL and assess its prognostic value. A total of 63 formalin‑fixed paraffin‑embedded tissue samples from patients with primary OC were collected and analyzed in the present study. The methylation profiles of KL were assessed by performing DNA bisulfate treatment followed by DNA promoter methylation analysis using the MethyLight assay. The results revealed KL promoter hypermethylation in 62% of the OC cohort. Additionally, significant associations were observed between KL methylation profiles and tumor subtype (P<0.0001) and tumor site (P=0.039). Furthermore, Kaplan‑Meier analysis revealed that a worse disease‑specific survival was significantly associated with hypermethylated KL (P=0.03, log‑rank; hazard ration, 0.58; 95% confidence interval (CI), 0.26‑0.90). Cox regression multivariate analysis indicated that KL promoter methylation was an independent OC prognostic indicator (P=0.029). The current study suggested that KL may be a novel biomarker to predict prognosis in patients with OC, since patients with higher KL promoter methylation were more likely to have a poor prognosis and would therefore require frequent follow‑up and integrative personalized therapeutic approaches.
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Spandidos Publications style
Al‑Zahrani MH, Yahya FM, Assidi M, Dallol A and Buhmeida A: Klotho promoter methylation status and its prognostic value in ovarian cancer. Mol Clin Oncol 15: 181, 2021.
APA
Al‑Zahrani, M.H., Yahya, F.M., Assidi, M., Dallol, A., & Buhmeida, A. (2021). Klotho promoter methylation status and its prognostic value in ovarian cancer. Molecular and Clinical Oncology, 15, 181. https://doi.org/10.3892/mco.2021.2343
MLA
Al‑Zahrani, M. H., Yahya, F. M., Assidi, M., Dallol, A., Buhmeida, A."Klotho promoter methylation status and its prognostic value in ovarian cancer". Molecular and Clinical Oncology 15.3 (2021): 181.
Chicago
Al‑Zahrani, M. H., Yahya, F. M., Assidi, M., Dallol, A., Buhmeida, A."Klotho promoter methylation status and its prognostic value in ovarian cancer". Molecular and Clinical Oncology 15, no. 3 (2021): 181. https://doi.org/10.3892/mco.2021.2343
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Spandidos Publications style
Al‑Zahrani MH, Yahya FM, Assidi M, Dallol A and Buhmeida A: Klotho promoter methylation status and its prognostic value in ovarian cancer. Mol Clin Oncol 15: 181, 2021.
APA
Al‑Zahrani, M.H., Yahya, F.M., Assidi, M., Dallol, A., & Buhmeida, A. (2021). Klotho promoter methylation status and its prognostic value in ovarian cancer. Molecular and Clinical Oncology, 15, 181. https://doi.org/10.3892/mco.2021.2343
MLA
Al‑Zahrani, M. H., Yahya, F. M., Assidi, M., Dallol, A., Buhmeida, A."Klotho promoter methylation status and its prognostic value in ovarian cancer". Molecular and Clinical Oncology 15.3 (2021): 181.
Chicago
Al‑Zahrani, M. H., Yahya, F. M., Assidi, M., Dallol, A., Buhmeida, A."Klotho promoter methylation status and its prognostic value in ovarian cancer". Molecular and Clinical Oncology 15, no. 3 (2021): 181. https://doi.org/10.3892/mco.2021.2343
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