Dose‑dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high‑dose‑rate ionising radiation

  • Authors:
    • Satoru Monzen
    • Tatsuya Ueno
    • Mitsuru Chiba
    • Yuki Morino
    • Yasushi Mariya
    • Andrzej Wojcik
    • Lovisa Lundholm
  • View Affiliations

  • Published online on: November 23, 2021     https://doi.org/10.3892/mco.2021.2452
  • Article Number: 19
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Abstract

Biomarkers of tumour response to radiotherapy may help optimise cancer treatment. The aim of the present study was to identify changes in extracellular microRNAs (miRNAs) as a biomarker of radiation‑induced damage to human colorectal cancer cells. HCT116 cells were exposed to increasing doses of X‑rays, and extracellular miRNAs were analysed by microarray. The results were correlated with the frequency of micronuclei. A total of 59 miRNAs with a positive correlation and 4 with a negative correlation between dose (up to 6 Gy) and extracellular miRNA expression were identified. In addition, for doses between 0 and 10 Gy, 12 miRNAs among those 59 miRNAs with a positive correlation were identified; for these extracellular miRNAs, a significantly positive correlation was observed between their expression and the frequency of micronuclei for doses up to 10 Gy. These results suggest that specific miRNAs may be considered as cell damage markers and may serve as secreted radiotherapy response biomarkers for colorectal cancer; however, the results must be further validated in serum samples collected from patients undergoing radiotherapy.

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Spandidos Publications style
Monzen S, Ueno T, Chiba M, Morino Y, Mariya Y, Wojcik A and Lundholm L: Dose‑dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high‑dose‑rate ionising radiation. Mol Clin Oncol 16: 19, 2022
APA
Monzen, S., Ueno, T., Chiba, M., Morino, Y., Mariya, Y., Wojcik, A., & Lundholm, L. (2022). Dose‑dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high‑dose‑rate ionising radiation. Molecular and Clinical Oncology, 16, 19. https://doi.org/10.3892/mco.2021.2452
MLA
Monzen, S., Ueno, T., Chiba, M., Morino, Y., Mariya, Y., Wojcik, A., Lundholm, L."Dose‑dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high‑dose‑rate ionising radiation". Molecular and Clinical Oncology 16.1 (2022): 19.
Chicago
Monzen, S., Ueno, T., Chiba, M., Morino, Y., Mariya, Y., Wojcik, A., Lundholm, L."Dose‑dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high‑dose‑rate ionising radiation". Molecular and Clinical Oncology 16, no. 1 (2022): 19. https://doi.org/10.3892/mco.2021.2452