Associations between matrix metalloproteinase, tissue inhibitor of metalloproteinase and collagen expression levels in the adjacent rectal tissue of colorectal carcinoma patients

Dibdiakova,Katarina; Svec,Adam; Majercikova,Zuzana; Adamik,Marek; Grendar,Marian; Vana,Juraj; Ferko,Alexander; Hatok,Jozef

doi:10.3892/mco.2021.2475

Associations between matrix metalloproteinase, tissue inhibitor of metalloproteinase and collagen expression levels in the adjacent rectal tissue of colorectal carcinoma patients

Authors:
- Katarina Dibdiakova
- Adam Svec
- Zuzana Majercikova
- Marek Adamik
- Marian Grendar
- Juraj Vana
- Alexander Ferko
- Jozef Hatok
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Affiliations: Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, SK‑03601 Martin, Slovakia, Department of Surgery and Transplant Centre, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, SK‑03601 Martin, Slovakia, Department of Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, SK‑03601 Martin, Slovakia, Department of Surgery, The Faculty Hospital, SK‑01207 Zilina, Slovakia
Published online on: December 21, 2021 https://doi.org/10.3892/mco.2021.2475
Article Number: 41
Copyright: © Dibdiakova et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As the commonest type of cancer in Europe and the third most common type of cancer worldwide, colorectal carcinoma (CRC) poses a challenge for numerous scientific studies. At present, the cause of this disease is remains to be elucidated, but early diagnosis is only one solution to prevent serious health complications. As a structural scaffold, the extracellular matrix (ECM) is in direct contact with tumour cells and significantly interferes with tumour progression. During the process of tumorigenesis, the ECM undergoes structural changes in which collagens serve an important role. Their life cycle is regulated by proteolytic enzymes called matrix metalloproteinases (MMPs), which are controlled by tissue inhibitors of metalloproteinases (TIMPs). The present study analysed the gene expression of MMPs (MMP1‑2‑8‑10‑13), TIMPs (TIMP1‑2‑4) and collagens (COL1A1 and COL3A1) and the correlation with biochemical parameters in the adjacent rectal tissue (ART) of patients with CRC. The patients who underwent standard neoadjuvant pre‑therapy showed increased concentrations of collagen in the normal ART. The mRNA levels of COL3A1, TIMP1 and TIMP2 were significantly higher in the ART of CRC patients (with or without pre‑therapy) when compared with the control group. This finding suggested that TIMPs served an important role in the regulation of MMPs and in the modification of collagen content in the ECM. Despite the small data set, the present study provided insights into the transcriptomic relationships between the individual genes that are an integral part of the ECM.

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