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Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer

  • Authors:
    • Masaaki Ito
    • Yusuke Oji
    • Mayuko Adachi
    • Rin Imanishi
    • Shouq Alzaaqi
    • Takaki Hiwasa
    • Yoko Oshima
    • Satoshi Yajima
    • Takashi Suzuki
    • Tatsuki Nanami
    • Makoto Sumazaki
    • Fumiaki Shiratori
    • Kimihiko Funahashi
    • Haruo Sugiayama
    • Hideaki Shimada
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Oncology, Toho University Graduate School of Medicine, Tokyo 143‑8541, Japan, Department of Clinical Laboratory and Biomedical Sciences, Osaka University Graduate School of Medicine, Osaka 565‑0871, Japan, Department of Gastroenterological Surgery, Toho University School of Medicine, Tokyo 143‑8541, Japan, Department of Cancer Immunology, Osaka University Graduate School of Medicine, Osaka 565‑0871, Japan
    Copyright: © Ito et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 74
    |
    Published online on: February 1, 2022
       https://doi.org/10.3892/mco.2022.2507
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Abstract

The Wilms tumor 1 gene, WT1, is overexpressed in various types of cancer, including gastric cancer. The product of WT1 is highly immunogenic and is a promising target molecule for cancer immunotherapy. The current study aimed to examine the production of WT1‑specific IgG and IgM autoantibodies to identify biomarkers of diagnostic value in patients with gastric cancer. IgG antibodies that bind to WT1‑derived peptides were obtained, the serum levels of which correlate with those of IgG antibodies against the WT1 protein in patients with intestinal malignancies. The serum levels of IgG and IgM antibodies against the WT1‑271 peptide (271‑288 amino acids) were examined in 39 healthy individuals and 97 patients with gastric cancer. The positivity cutoff value was determined according to the receiver operating characteristic curve. The association between WT1‑271 IgM and the clinicopathological factors and prognosis of patients was additionally analyzed. The results revealed that serum WT1‑271 IgM antibody levels in patients with gastric cancer were significantly higher than those in healthy individuals. The sensitivity and specificity of this antibody for gastric cancer were 67.0 and 71.8%, respectively; this sensitivity was improved when compared with conventional tumor markers (P<0.001). There was no statistical difference in WT1‑271 IgG antibody levels between patients with gastric cancer and healthy individuals. Serum WT1‑271 IgM antibody levels were not significantly associated with clinicopathological factors but were associated with unfavorable prognosis. Serum WT1‑271 IgM antibody levels could serve as a diagnostic biomarker in patients with gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ito M, Oji Y, Adachi M, Imanishi R, Alzaaqi S, Hiwasa T, Oshima Y, Yajima S, Suzuki T, Nanami T, Nanami T, et al: Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer. Mol Clin Oncol 16: 74, 2022.
APA
Ito, M., Oji, Y., Adachi, M., Imanishi, R., Alzaaqi, S., Hiwasa, T. ... Shimada, H. (2022). Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer. Molecular and Clinical Oncology, 16, 74. https://doi.org/10.3892/mco.2022.2507
MLA
Ito, M., Oji, Y., Adachi, M., Imanishi, R., Alzaaqi, S., Hiwasa, T., Oshima, Y., Yajima, S., Suzuki, T., Nanami, T., Sumazaki, M., Shiratori, F., Funahashi, K., Sugiayama, H., Shimada, H."Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer". Molecular and Clinical Oncology 16.3 (2022): 74.
Chicago
Ito, M., Oji, Y., Adachi, M., Imanishi, R., Alzaaqi, S., Hiwasa, T., Oshima, Y., Yajima, S., Suzuki, T., Nanami, T., Sumazaki, M., Shiratori, F., Funahashi, K., Sugiayama, H., Shimada, H."Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer". Molecular and Clinical Oncology 16, no. 3 (2022): 74. https://doi.org/10.3892/mco.2022.2507
Copy and paste a formatted citation
x
Spandidos Publications style
Ito M, Oji Y, Adachi M, Imanishi R, Alzaaqi S, Hiwasa T, Oshima Y, Yajima S, Suzuki T, Nanami T, Nanami T, et al: Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer. Mol Clin Oncol 16: 74, 2022.
APA
Ito, M., Oji, Y., Adachi, M., Imanishi, R., Alzaaqi, S., Hiwasa, T. ... Shimada, H. (2022). Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer. Molecular and Clinical Oncology, 16, 74. https://doi.org/10.3892/mco.2022.2507
MLA
Ito, M., Oji, Y., Adachi, M., Imanishi, R., Alzaaqi, S., Hiwasa, T., Oshima, Y., Yajima, S., Suzuki, T., Nanami, T., Sumazaki, M., Shiratori, F., Funahashi, K., Sugiayama, H., Shimada, H."Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer". Molecular and Clinical Oncology 16.3 (2022): 74.
Chicago
Ito, M., Oji, Y., Adachi, M., Imanishi, R., Alzaaqi, S., Hiwasa, T., Oshima, Y., Yajima, S., Suzuki, T., Nanami, T., Sumazaki, M., Shiratori, F., Funahashi, K., Sugiayama, H., Shimada, H."Serum WT1‑271 IgM antibody as a novel diagnostic marker for Gastric Cancer". Molecular and Clinical Oncology 16, no. 3 (2022): 74. https://doi.org/10.3892/mco.2022.2507
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