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Article Open Access

Size‑based isolation and detection of renal carcinoma cells from whole blood

  • Authors:
    • Roger De Alwis
    • Jennifer Hansson
    • David Lindgren
    • Sarah Schoch
    • Alexander Tejera
    • Bianca Scholtz
    • Peter Elfving
    • Christina Möller
    • Helén Nilsson
    • Martin Johansson
    • Håkan Axelson
  • View Affiliations / Copyright

    Affiliations: Division of Translational Cancer Research, Department of Laboratory Medicine, Lund University, 223 81 Lund, Sweden, Department of Urology, Skåne University Hospital, 214 28 Malmö, Sweden, Center for Molecular Pathology, Department of Translational Medicine, Skåne University Hospital, 214 28 Malmö, Sweden
    Copyright: © De Alwis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 101
    |
    Published online on: April 7, 2022
       https://doi.org/10.3892/mco.2022.2534
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Abstract

Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics‑based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked‑in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC‑specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.
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Copy and paste a formatted citation
Spandidos Publications style
De Alwis R, Hansson J, Lindgren D, Schoch S, Tejera A, Scholtz B, Elfving P, Möller C, Nilsson H, Johansson M, Johansson M, et al: Size‑based isolation and detection of renal carcinoma cells from whole blood. Mol Clin Oncol 16: 101, 2022.
APA
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B. ... Axelson, H. (2022). Size‑based isolation and detection of renal carcinoma cells from whole blood. Molecular and Clinical Oncology, 16, 101. https://doi.org/10.3892/mco.2022.2534
MLA
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B., Elfving, P., Möller, C., Nilsson, H., Johansson, M., Axelson, H."Size‑based isolation and detection of renal carcinoma cells from whole blood". Molecular and Clinical Oncology 16.5 (2022): 101.
Chicago
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B., Elfving, P., Möller, C., Nilsson, H., Johansson, M., Axelson, H."Size‑based isolation and detection of renal carcinoma cells from whole blood". Molecular and Clinical Oncology 16, no. 5 (2022): 101. https://doi.org/10.3892/mco.2022.2534
Copy and paste a formatted citation
x
Spandidos Publications style
De Alwis R, Hansson J, Lindgren D, Schoch S, Tejera A, Scholtz B, Elfving P, Möller C, Nilsson H, Johansson M, Johansson M, et al: Size‑based isolation and detection of renal carcinoma cells from whole blood. Mol Clin Oncol 16: 101, 2022.
APA
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B. ... Axelson, H. (2022). Size‑based isolation and detection of renal carcinoma cells from whole blood. Molecular and Clinical Oncology, 16, 101. https://doi.org/10.3892/mco.2022.2534
MLA
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B., Elfving, P., Möller, C., Nilsson, H., Johansson, M., Axelson, H."Size‑based isolation and detection of renal carcinoma cells from whole blood". Molecular and Clinical Oncology 16.5 (2022): 101.
Chicago
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B., Elfving, P., Möller, C., Nilsson, H., Johansson, M., Axelson, H."Size‑based isolation and detection of renal carcinoma cells from whole blood". Molecular and Clinical Oncology 16, no. 5 (2022): 101. https://doi.org/10.3892/mco.2022.2534
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