Open Access

Size‑based isolation and detection of renal carcinoma cells from whole blood

  • Authors:
    • Roger De Alwis
    • Jennifer Hansson
    • David Lindgren
    • Sarah Schoch
    • Alexander Tejera
    • Bianca Scholtz
    • Peter Elfving
    • Christina Möller
    • Helén Nilsson
    • Martin Johansson
    • Håkan Axelson
  • View Affiliations

  • Published online on: April 7, 2022     https://doi.org/10.3892/mco.2022.2534
  • Article Number: 101
  • Copyright: © De Alwis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the observation that RCC tumour cells are considerably larger than leucocytes, the present study employed a microfluidics‑based system for isolation of RCC CTCs from whole blood. Using this system, it was revealed that 66% of spiked‑in RCC tumour cells could be retrieved using this approach. Furthermore, it was demonstrated that these cells could be molecularly detected with digital PCR using RCC‑specific genes down to one tumour cell, whilst avoiding detection in samples lacking tumour cells. Finally, subtype specific transcripts were identified to distinguish the different subtypes of RCC, which were then validated in patient tumours. The present study established a novel workflow for the isolation of RCC CTCs from whole blood, with the potential to detect these cells irrespective of subtype.
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May-2022
Volume 16 Issue 5

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
De Alwis R, Hansson J, Lindgren D, Schoch S, Tejera A, Scholtz B, Elfving P, Möller C, Nilsson H, Johansson M, Johansson M, et al: Size‑based isolation and detection of renal carcinoma cells from whole blood. Mol Clin Oncol 16: 101, 2022
APA
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B. ... Axelson, H. (2022). Size‑based isolation and detection of renal carcinoma cells from whole blood. Molecular and Clinical Oncology, 16, 101. https://doi.org/10.3892/mco.2022.2534
MLA
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B., Elfving, P., Möller, C., Nilsson, H., Johansson, M., Axelson, H."Size‑based isolation and detection of renal carcinoma cells from whole blood". Molecular and Clinical Oncology 16.5 (2022): 101.
Chicago
De Alwis, R., Hansson, J., Lindgren, D., Schoch, S., Tejera, A., Scholtz, B., Elfving, P., Möller, C., Nilsson, H., Johansson, M., Axelson, H."Size‑based isolation and detection of renal carcinoma cells from whole blood". Molecular and Clinical Oncology 16, no. 5 (2022): 101. https://doi.org/10.3892/mco.2022.2534