Methanol extraction revealed anticancer compounds Quinic Acid, 2(5H)‑Furanone and Phytol in <em>Andrographis paniculata</em>

Anoor,Pawan Kumar; Anoor,Pawan Kumar; Yadav,A Nichita; Yadav,A Nichita; Rajkumar,Karthik; Rajkumar,Karthik; Kande,Ramesh; Kande,Ramesh; Tripura,Chaturvedula; Tripura,Chaturvedula; Naik,K Srinivas; Naik,K Srinivas; Burgula,Sandeepta; Burgula,Sandeepta

doi:10.3892/mco.2022.2584

Methanol extraction revealed anticancer compounds Quinic Acid, 2(5H)‑Furanone and Phytol in Andrographis paniculata

Authors:
- Pawan Kumar Anoor
- A Nichita Yadav
- Karthik Rajkumar
- Ramesh Kande
- Chaturvedula Tripura
- K Srinivas Naik
- Sandeepta Burgula
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Affiliations: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana 500007, India, CSIR‑Centre for Cellular and Molecular Biology Annexe‑II, Medical Biotechnology Campus, Annexe‑II, Hyderabad, Telangana 500007, India, Centre for Plant Molecular Biology, Osmania University, Hyderabad, Telangana 500007, India
Published online on: September 13, 2022 https://doi.org/10.3892/mco.2022.2584
Article Number: 151
Copyright: © Anoor et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Andrographis paniculata (Ap) has been a part of traditional medicine for its anti‑inflammatory effects, treatment of snake bites and liver abnormalities. Several investigations have revealed its bioactive components to be andrographolides. The methanolic extracts of leaves from Ap were characterized, the non‑andrographolides were identified and screened for anti‑proliferative activity. The extracts showed significant toxicity against numerous cancer cells including HeLa, MCF7, BT549, 293 and A549 cells. Anti‑proliferative activity and effect on cancer cell invasion (metastatic potential) and cell migration were examined. The extracts revealed significant inhibition of the ability of HeLa cells in repairing the gap created by a vertical wound made on a confluent cell monolayer. Similarly, a 40% reduction in cell migration was observed in presence of the extracts. Significant anti‑angiogenic activity in terms of reduced blood capillary formation was observed on the Chorioallantoic membranes of embryonated hen eggs co‑inoculated with HeLa cells and the extracts. Analysis of HeLa cells treated with the extracts using flow cytometry indicated the arrest of cell cycle progression at the G2/M phase. Variation in the Bax/Bcl‑2 ratio and elevated caspase‑3 levels by immunoblotting confirmed cell death induction via the apoptotic pathway. Investigation of the extracts by gas chromatography‑mass spectrometry displayed the predominant components to be 2(5H)‑Furanone (14.73%), Quinic acid (17.32%), and Phytol (11.43%). These components have been previously known to have anticancer activity, while being studied individually in other plants. This is the first study, to the best of our knowledge, on the anti‑proliferative and anti‑angiogenic activity of the non‑andrographolide components from Ap.

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