Open Access

Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

  • Authors:
    • Yuqian Wu
    • Qin Liu
    • Jiaojiao Tong
    • Chunhui Hu
    • Dianshui Sun
  • View Affiliations

  • Published online on: February 3, 2023     https://doi.org/10.3892/mco.2023.2617
  • Article Number: 21
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Extensive neck lymph node metastasis (LNM) is an important clinical feature of hypopharyngeal squamous cell carcinoma (HSCC). Stathmin1 (STMN1) is closely associated with LNM in numerous human cancers. In the present study, the association between STMN1 and neck LNM in HSCC and the underlying molecular mechanisms were explored. First, postoperative samples of HSCC were screened and the association between STMN1 and neck LNM in HSCC was analyzed. Then, cell functional experiments were performed to assess the potential of STMN1 to promote invasion and migration. Subsequently, the potential target genes and pathways of STMN1 were predicted using bioinformatics analysis. Finally, the obtained target genes and pathways of STMN1 were validated by reverse transcription‑quantitative PCR (RT‑qPCR) and western blot analyses to confirm the potential mechanisms by which STMN1 promotes LNM in HSCC. As a result, a total of 117 postoperative samples of HSCC were screened, and STMN1 was proven to be associated with neck LNM in HSCC. Further, cell functional experiments established that high expression of STMN1 could actually promote FaDu cell invasion and metastasis. Bioinformatics analysis revealed that high expression of STMN1 was associated with the activation of hypoxia inducible factor‑1alpha (HIF‑1α) pathway and increased expression of metastasis‑associated protein 1 (MTA1). Finally, RT‑qPCR and western blot analyses confirmed that STMN1 promotes the expression levels of HIF‑1α/vascular endothelial growth factor (VEGF)‑A and MTA1 in FaDu cell lines. In conclusion, it was found that high expression of STMN1 promoted neck LNM in HSCC and the potential mechanisms may be via regulation of the HIF‑1α/VEGF‑A axis and MTA1 expression.
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March-2023
Volume 18 Issue 3

Print ISSN: 2049-9450
Online ISSN:2049-9469

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Spandidos Publications style
Wu Y, Liu Q, Tong J, Hu C and Sun D: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression. Mol Clin Oncol 18: 21, 2023
APA
Wu, Y., Liu, Q., Tong, J., Hu, C., & Sun, D. (2023). Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression. Molecular and Clinical Oncology, 18, 21. https://doi.org/10.3892/mco.2023.2617
MLA
Wu, Y., Liu, Q., Tong, J., Hu, C., Sun, D."Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression". Molecular and Clinical Oncology 18.3 (2023): 21.
Chicago
Wu, Y., Liu, Q., Tong, J., Hu, C., Sun, D."Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression". Molecular and Clinical Oncology 18, no. 3 (2023): 21. https://doi.org/10.3892/mco.2023.2617