Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular and Clinical Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 2049-9450 Online ISSN: 2049-9469
Journal Cover
August-2025 Volume 23 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2025 Volume 23 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Nuclear factor IA‑mediated transcriptional regulation of crystallin αB inhibits hepatocellular carcinoma progression

  • Authors:
    • Yun Jin
    • Pingping Hu
    • Yihe Dai
    • Wenchao Gu
    • Jiang Han
    • Haihan Song
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The First People's Hospital of Yunnan Province, Kunming, Yunnan 650032, P.R. China, Department of Hepatobiliary Surgery, The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan 650032, P.R. China, Department of Pulmonary and Critical Care Medicine, Shanghai Pudong New Area People's Hospital, Shanghai 201299, P.R. China, Central Laboratory, Shanghai Key Laboratory of Pathogenic Fungi Medical Testing, Shanghai Pudong New Area People's Hospital, Shanghai 201299, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 72
    |
    Published online on: June 20, 2025
       https://doi.org/10.3892/mco.2025.2867
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hepatocellular carcinoma (HCC) is a highly invasive malignant tumor with limited therapeutic options. In the present study, bioinformatics analysis, including differential expression analysis, functional enrichment, protein‑protein interaction network construction, survival analysis and risk model evaluation, identified CRYAB as a central prognostic gene in HCC. Additionally, motif analysis using JASPAR revealed nuclear factor IA (NFIA), as a potential transcriptional regulator of CRYAB. Further in vitro experiments were conducted to explore the roles of CRYAB and NFIA in HCC, suggesting that these molecules may serve as promising therapeutic targets for future research. Differentially expressed genes (DEGs) from the Cancer Genome Atlas‑liver hepatocellular carcinoma (LIHC) and GSE113996 datasets were identified using the ‘limma’ package, with Biological Process and Kyoto Encyclopedia of Genes and Genomes enrichment analysis conducted. Overlapping DEGs underwent Protein‑protein interaction and prognostic analysis. Key prognostic genes were selected through Kaplan‑Meier survival analysis and Least Absolute Shrinkage and Selection Operator regression before they were incorporated into a predictive risk model, which was evaluated by receiver operating characteristic analysis. JASPAR motif analysis identified NFIA as a potential transcriptional regulator of CRYAB, with the TIMER database used to further examine the NFIA expression profile among other cancers. In vitro assays using MHCC97H and Huh7 cells were used to examine the roles of CRYAB and NFIA in HCC. Cell counting kit‑8 (CCK‑8) assay was used to assess proliferation, whilst Transwell assay was used to measure migration and invasion. To investigate the reciprocal regulation, rescue experiments combining NFIA overexpression and CRYAB knockdown were performed to compare their effects on cell proliferation, migration and invasion. Additionally, dual‑luciferase assay was used to examine the regulatory effect of NFIA on the CRYAB promoter by comparing the wild‑type and mutant constructs. Bioinformatics analyses revealed CRYAB to be a hub gene. CRYAB upregulation was found to be associated with poor prognosis in patients with LIHC. In vitro, elevated CRYAB expression was observed in HCC cell lines compared with that in the huma liver immortalized cell line THLE‑2. CRYAB knockdown was found to significantly inhibit MHCC97H and Huh7 cell proliferation, migration and invasion. By contrast, NFIA expression was found to be downregulated in LIHC compared with that in normal liver tissues, where its expression showed an inverse association with that of CRYAB. Direct interaction between NFIA and the CRYAB promoter region was confirmed through dual‑luciferase assays. Furthermore, low NFIA expression markedly enhanced HCC cell proliferation, invasion and migration. This pro‑tumor effect was reversed in the si‑NFIA + si‑CRYAB group, where simultaneous downregulation of CRYAB significantly reduced cell proliferation, migration and invasion, suggesting that CRYAB downregulation can counteract the effects induced by low NFIA expression. To conclude, these results suggest that NFIA can inhibit the malignant proliferation of HCC cells by activating CRYAB expression, which further suggest that CRYAB and NFIA are promising avenues for the development of novel HCC treatment strategies.
View Figures

Figure 1

Comprehensive analysis of
differentially expressed genes and pathway enrichment. Volcano maps
of DEGs based on the (A) TCGA and (B) GSE113996 datasets. Gray
scatter points represent genes with insignificant differences in
expression, whilst orange and green scatter points represent genes
with significant differences in expression. X-axis shows
log2 fold-change and Y-axis shows -log10
P-value. (C) Venn diagram showing the overlapping DEGs between
TCGA-LIHC and GSE113996 datasets. Chord diagram showing enrichment
analysis of (D) BP and (E) KEGG. The right ring of the outermost
circle shows 10 BP terms, each term is identified by a color. The
left ring of the outermost circle shows the gene column, the
gradient color indicates the gene differential expression fold
change. Inner circles show associations between genes and terms.
BP, biological process; DEGs, differentially expressing genes;
TCGA, The Cancer Genome Atlas; up, upregulated; down,
downregulated.

Figure 2

PPI network analysis and prognostic
significance of overlapping genes in liver cancer. (A) PPI network
representation of 42 overlapping genes. Nodes represent proteins or
protein domains, while edges represent interactions between these
proteins, with edge thickness representing the confidence of the
interaction. Kaplan-Meier survival curves for the identified
prognostically significant genes: (B) AKR1B10, (C) AKR1B15, (D)
CRYAB, (E) DLAT, (F) HSPB8, (G) IFITM1, (H) NSDHL, (I) SQLE and (J)
STC2. The x-axis represents survival time in months and the y-axis
represents survival probability. HR represents the risk of an event
in the high-risk group compared to the low-risk group. Log-rank
P-value indicates the statistical significance of the difference in
survival between high-risk and low-risk groups. PPI,
protein-protein interaction; HR, hazard ratio; AKR1B10, aldo-keto
reductase family 1, member B10; AKR1B15, aldo-keto reductase 1B15;
CRYAB, Crystallin αB; DLAT, dihydrolipoamide S-acetyltransferase;
HSPB8, heat shock protein family B member 8; IFITM1, interferon
induced transmembrane protein 1; NSDHL, NAD(P)-dependent steroid
dehydrogenase-like; SQLE, squalene epoxidase; STC2, stanniocalcin
2.

Figure 3

Risk prediction model analysis and
characterization of prognostic genes. (A) LASSO regression analysis
highlighting the coefficients of the eight prognosis-associated
genes against the L1 Norm. (B) Cross-validation utilized for tuning
parameter determination in the LASSO regression model. The x-axis
represents the log(λ) value and the y-axis indicates partial
likelihood deviance. (C) Risk score distribution of patients with
liver cancer, segregated into low-risk and high-risk categories.
The upper scatterplot elucidates the association between risk
scores and patient survival status and duration. The lower plot
shows a heatmap of z-scores of normalized expression levels of the
prognostic genes across the risk stratifications. The x-axis in the
upper scatterplot shows ‘patient’ identifiers, each representing an
individual's risk score and survival data. In the lower heatmap
below, the x-axis shows the ‘genes’ involved in liver cancer
prognosis, with their expression levels depicted across the
different risk categories. (D) Kaplan-Meier survival analysis
contrasting the overall survival between low- and high-risk
cohorts. The x-axis chronicles the time in years, whereas the
y-axis plots the overall survival probability. (E) Receiver
operating characteristic curves demonstrating the predictive
performance of the prognostic signature at 1-year, 3-year, and
5-year intervals. HR, hazard ratio; AUC, area under the curve;
LASSO, Least Absolute Shrinkage and Selection Operator; NSDHL,
NAD(P)-dependent steroid dehydrogenase-like; IFITM1, interferon
induced transmembrane protein 1; DLAT, dihydrolipoamide
S-acetyltransferase; STC2, stanniocalcin 2; HSPB8, heat shock
protein family B member 8; AKR1B15, aldo-keto reductase 1B15;
CRYAB, crystallin αB.

Figure 4

Regulation of liver cancer cell
proliferation, migration and invasion by CRYAB knockdown. (A)
RT-qPCR and (B) WB analysis of CRYAB expression in THLE-2 cells and
4 liver cancer cell lines (HepG2, MHCC97H, Huh7 and Hep3B).
*P<0.05 vs. THLE-2. (C) RT-qPCR and (D) WB were used
to evaluate the efficiency of CRYAB knockdown in MHCC97H and Huh7
cells. *P<0.05 vs. THLE-2. Cell Counting Kit-8 assay
of the effect of CRYAB knockdown on (E) MHCC97H and (F) cell
proliferation. *P<0.05 vs. si-NC. Transwell assay to
assess the effect of CRYAB knockdown on migration and invasion of
(G) MHCC97H cells and (H) were semi-quantified (scale bars, 50 µm).
Transwell assay to assess the effect of CRYAB knockdown on
migration and invasion of (I) Huh7 cells and (J) were
semi-quantified. *P<0.05 vs. si-NC. CRYAB, Crystallin
αB; RT-qPCR, reverse transcription-quantitative PCR; WB, western
blotting; si, small interfering; NC, negative control; OD, optical
density.

Figure 5

NFIA activates CRYAB gene expression
by binding to its promoter region. (A) Expression level analysis of
NFIA in pan-cancer using TIMER database. *P<0.05,
**P<0.01 and ***P<0.001. (B) Western
blotting analysis of NFIA and CRYAB expression in liver cell lines
after NFIA overexpression (using NFIA overexpression plasmid,
labeled as ‘over-NFIA’) and knockdown. ‘Vector’ refers to the
control plasmid used in the overexpression experiments, whilst
‘si-NC’ represents the negative control for siRNA knockdown
experiments. (C) Upper figure shows JASPAR software detecting
potential NFIA binding sites in the upstream genome sequence of the
CRYAB coding region, lower figure shows the structure diagram of
reporter genes based on the pGL3-Basic vector in luciferase
detection. Dual-luciferase reporter assay in 293T cells measuring
changes in wild-type and mutant luciferase activities after NFIA
(D) overexpression or (E) knockdown. *P<0.05 vs.
over-NC or si-NC. TPM, transcript per million; CRYAB, Crystallin
αB; NFIA, nuclear factor IA; WT, wild-type; MUT, mutant; over-,
overexpression plasmid; si-small interfering; NC, negative
control.

Figure 6

Role of NFIA and CRYAB in liver
cancer cell proliferation, migration and invasion. Cell Counting
Kit-8 was used to measure (A) MHCC97H and (B) Huh7 cell
proliferation after the knockdown of NFIA and/or CRYAB.
*P<0.05 vs. si-NC. Transwell assay was used to
measure cell migration and invasion after combined knockdown of
NFIA and/or CRYAB in (C) MHCC97H cells, (D) which was
semi-quantified. Transwell assay was used to measure cell migration
and invasion after combined knockdown of NFIA and/or CRYAB in (E)
Huh7 cells, (F) which was semi-quantified. Scale bars, 50 µm.
*P<0.05 vs. si-NC. #P<0.05 vs. si-NFIA.
CRYAB, Crystallin αB; NFIA, nuclear factor IA; si, small
interferin; NC, negative control.
View References

1 

Qiu S, Cai J, Yang Z, He X, Xing Z, Zu J, Xie E, Henry L, Chong CR, John EM, et al: Trends in Hepatocellular Carcinoma Mortality Rates in the US and Projections Through 2040. JAMA Netw Open. 7(e2445525)2024.PubMed/NCBI View Article : Google Scholar

2 

Giri S and Singh A: Epidemiology of Hepatocellular Carcinoma in India-An Updated Review for 2024. J Clin Exp Hepatol. 14(101447)2024.PubMed/NCBI View Article : Google Scholar

3 

Hahn JW, Woo S, Park J, Lee H, Kim HJ, Ko JS, Moon JS, Rahmati M, Smith L, Jang J, et al: Global, Regional, and National Trends in Liver Disease-Related Mortality Across 112 Countries From 1990 to. 2021, With Projections to 2050: Comprehensive Analysis of the WHO Mortality Database. J Korean Med Sci. 39(e292)2024.PubMed/NCBI View Article : Google Scholar

4 

Sagnelli E, Macera M, Russo A, Coppola N and Sagnelli C: Epidemiological and etiological variations in hepatocellular carcinoma. Infection. 48:7–17. 2020.PubMed/NCBI View Article : Google Scholar

5 

McGlynn KA, Petrick JL and El-Serag HB: Epidemiology of hepatocellular carcinoma. Hepatology. 73:4–13. 2021.PubMed/NCBI View Article : Google Scholar

6 

Pandyarajan V, Govalan R and Yang JD: Risk factors and biomarkers for chronic hepatitis B associated hepatocellular carcinoma. Int J Mol Sci. 22(479)2021.PubMed/NCBI View Article : Google Scholar

7 

Li W and Ni CF: Current status of the combination therapy of transarterial chemoembolization and local ablation for hepatocellular carcinoma. Abdom Radiol (NY). 44:2268–2275. 2019.PubMed/NCBI View Article : Google Scholar

8 

Viveiros P, Riaz A, Lewandowski RJ and Mahalingam D: Current state of liver-directed therapies and combinatory approaches with systemic therapy in hepatocellular carcinoma (HCC). Cancers (Basel). 11(1085)2019.PubMed/NCBI View Article : Google Scholar

9 

Nazzal M, Gadani S, Said A, Rice M, Okoye O, Taha A and Lentine KL: Liver targeted therapies for hepatocellular carcinoma prior to transplant: Contemporary management strategies. Glob Surg Feb. 15:2018.PubMed/NCBI View Article : Google Scholar : (Epub ahead of print).

10 

Fuster-Anglada C, Mauro E, Ferrer-Fàbrega J, Caballol B, Sanduzzi-Zamparelli M, Bruix J, Fuster J, Reig M, Díaz A and Forner A: Histological predictors of aggressive recurrence of hepatocellular carcinoma after liver resection. J Hepatol. 81:995–1004. 2024.PubMed/NCBI View Article : Google Scholar

11 

Glavas D, Bao QR, Scarpa M, Ruffolo C, Brown ZJ, Pawlik TM and Spolverato G: Treatment and prognosis of fibrolamellar hepatocellular carcinoma: A systematic review of the recent literature and meta-analysis. J Gastrointest Surg. 27:705–715. 2023.PubMed/NCBI View Article : Google Scholar

12 

Xu Y, Zhang X, Zhang R, Sun Y, Liu J, Luo C, Yang J, Fang W, Guo Q and Wei L: AFP deletion leads to anti-tumorigenic but pro-metastatic roles in liver cancers with concomitant CTNNB1 mutations. Cancer Lett. 566(216240)2023.PubMed/NCBI View Article : Google Scholar

13 

Zheng Y, Zhu M and Li M: Effects of alpha-fetoprotein on the occurrence and progression of hepatocellular carcinoma. J Cancer Res Clin Oncol. 146:2439–2446. 2020.PubMed/NCBI View Article : Google Scholar

14 

Xu W, Guo Y, Huang Z, Zhao H, Zhou M, Huang Y, Wen D, Song J, Zhu Z, Sun M, et al: Small heat shock protein CRYAB inhibits intestinal mucosal inflammatory responses and protects barrier integrity through suppressing IKKβ activity. Mucosal Immunol. 12:1291–1303. 2019.PubMed/NCBI View Article : Google Scholar

15 

Yin B, Tang S, Xu J, Sun J, Zhang X, Li Y and Bao E: CRYAB protects cardiomyocytes against heat stress by preventing caspase-mediated apoptosis and reducing F-actin aggregation. Cell Stress Chaperones. 24:59–68. 2019.PubMed/NCBI View Article : Google Scholar

16 

Huang Z, Mou T, Luo Y, Pu X, Pu J, Wan L, Gong J, Yang H, Liu Y, Li Z, et al: Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB. Cell Death Dis. 11(455)2020.PubMed/NCBI View Article : Google Scholar

17 

Zhang J, Liu J, Wu J, Li W, Chen Z and Yang L: Progression of the role of CRYAB in signaling pathways and cancers. Onco Targets Ther. 12:4129–4139. 2019.PubMed/NCBI View Article : Google Scholar

18 

Fane M, Harris L, Smith AG and Piper M: Nuclear factor one transcription factors as epigenetic regulators in cancer. Int J Cancer. 140:2634–2641. 2017.PubMed/NCBI View Article : Google Scholar

19 

Wu YZ, Chan KYY, Leung KT, Lam HS, Tam YH, Lee KH, Li K and Ng PC: The miR-223/nuclear factor I-A axis regulates inflammation and cellular functions in intestinal tissues with necrotizing enterocolitis. FEBS Open Bio. 11:1907–1920. 2021.PubMed/NCBI View Article : Google Scholar

20 

Lee J, Hoxha E and Song HR: A novel NFIA-NFκB feed-forward loop contributes to glioblastoma cell survival. Neuro Oncol. 19:524–534. 2017.PubMed/NCBI View Article : Google Scholar

21 

Liu C, Duan P, Li B, Huang C, Jing Y and Yan W: miR-29a activates Hes1 by targeting Nfia in esophageal carcinoma cell line TE-1. Oncol Lett. 9:96–102. 2015.PubMed/NCBI View Article : Google Scholar

22 

Kaczorowski M, Matysiak J, Kielb P, Malkiewicz B and Halon A: Nuclear Factor IA Is Down-regulated in Muscle-invasive and High-grade Bladder Cancers. Anticancer Res. 42:493–500. 2022.PubMed/NCBI View Article : Google Scholar

23 

Chen M, Wu GB, Xie ZW, Shi DL and Luo M: A novel diagnostic four-gene signature for hepatocellular carcinoma based on artificial neural network: Development, validation, and drug screening. Front Genet. 13(942166)2022.PubMed/NCBI View Article : Google Scholar

24 

Jin Y, Dai Y, Qiao O, Hu P and Han J: miR-1972 inhibits hepatocellular carcinoma proliferation by targeting GZMH-mediated DNA replication in the cell cycle. J Pharm Pharmacol: Apr 18, 2024 (Epub ahead of print).

25 

Xie Z, Huang J, Li Y, Zhu Q, Huang X, Chen J, Wei C, Luo S, Yang S and Gao J: Single-cell RNA sequencing revealed potential targets for immunotherapy studies in hepatocellular carcinoma. Sci Rep. 13(18799)2023.PubMed/NCBI View Article : Google Scholar

26 

Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W and Smyth GK: limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43(7)2015.doi: 10.1093/nar/gkv007.

27 

Jia A, Xu L and Wang Y: Venn diagrams in bioinformatics. Brief Bioinform. 22(bbab108)2021.PubMed/NCBI View Article : Google Scholar

28 

Yu G, Wang LG, Han Y and He QY: clusterProfiler: An R package for comparing biological themes among gene clusters. OMICS. 16:284–287. 2012.PubMed/NCBI View Article : Google Scholar

29 

Zhang S, Shi YN, Gu J, He P, Ai QD, Zhou XD, Wang W and Qin L: Mechanisms of dihydromyricetin against hepatocellular carcinoma elucidated by network pharmacology combined with experimental validation. Pharm Biol. 61:1108–1119. 2023.PubMed/NCBI View Article : Google Scholar

30 

Wang H, Cheng W, Hu P, Ling T, Hu C, Chen Y, Zheng Y, Wang J, Zhao T and You Q: Integrative analysis identifies oxidative stress biomarkers in non-alcoholic fatty liver disease via machine learning and weighted gene co-expression network analysis. Front Immunol. 15(1335112)2024.PubMed/NCBI View Article : Google Scholar

31 

Jin AL, Zhang CY, Zheng WJ, Xian JR, Yang WJ, Liu T, Chen W, Li T, Wang BL, Pan BS, et al: CD155/SRC complex promotes hepatocellular carcinoma progression via inhibiting the p38 MAPK signalling pathway and correlates with poor prognosis. Clin Transl Med. 12(e794)2022.PubMed/NCBI View Article : Google Scholar

32 

Ballout N, Etievant L and Viallon V: On the use of cross-validation for the calibration of the adaptive lasso. Biom J. 65(2200047)2023.PubMed/NCBI View Article : Google Scholar

33 

Lee M, Teber ET, Holmes O, Nones K, Patch AM, Dagg RA, Lau LMS, Lee JH, Napier CE, Arthur JW, et al: Telomere sequence content can be used to determine ALT activity in tumours. Nucleic Acids Res. 46:4903–4918. 2018.PubMed/NCBI View Article : Google Scholar

34 

Yan C, Niu Y, Ma L, Tian L and Ma J: System analysis based on the cuproptosis-related genes identifies LIPT1 as a novel therapy target for liver hepatocellular carcinoma. J Transl Med. 20(452)2022.PubMed/NCBI View Article : Google Scholar

35 

Koch DT, Yu H, Beirith I, Schirren M, Drefs M, Liu Y, Knoblauch M, Koliogiannis D, Sheng W, De Toni EN, et al: Tigecycline causes loss of cell viability mediated by mitochondrial OXPHOS and RAC1 in hepatocellular carcinoma cells. J Transl Med. 21(876)2023.PubMed/NCBI View Article : Google Scholar

36 

Zhao S, Zhang Y, Lu X, Ding H, Han B, Song X, Miao H, Cui X, Wei S, Liu W, et al: CDC20 regulates the cell proliferation and radiosensitivity of P53 mutant HCC cells through the Bcl-2/Bax pathway. Int J Biol Sci. 17:3608–3621. 2021.PubMed/NCBI View Article : Google Scholar

37 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

38 

Kong J, Wang X, Wang J and Yu G: Silencing of RAB42 down-regulated PD-L1 expression to inhibit the immune escape of hepatocellular carcinoma cells through inhibiting the E2F signaling pathway. Cell Signal. 108(110692)2023.PubMed/NCBI View Article : Google Scholar

39 

Fornes O, Castro-Mondragon JA, Khan A, Van der Lee R, Zhang X, Richmond PA, Modi BP, Correard S, Gheorghe M, Baranašić D, et al: JASPAR 2020: Update of the open-access database of transcription factor binding profiles. Nucleic Acids Res. 48(D1):D87–D92. 2020.PubMed/NCBI View Article : Google Scholar

40 

Zhong H, Shi Q, Wen Q, Chen J, Li X, Ruan R, Zeng S, Dai X, Xiong J, Li L, et al: Pan-cancer analysis reveals potential of FAM110A as a prognostic and immunological biomarker in human cancer. Front Immunol. 14(1058627)2023.PubMed/NCBI View Article : Google Scholar

41 

Hsiao CY, Chen PD and Huang KW: A prospective assessment of the diagnostic value of contrast-enhanced ultrasound, dynamic computed tomography and magnetic resonance imaging for patients with small liver tumors. J Clin Med. 8(1353)2019.PubMed/NCBI View Article : Google Scholar

42 

Park HJ, Jang HY, Kim SY, Lee SJ, Won HJ, Byun JH, Choi SH, Lee SS, An J and Lim YS: Non-enhanced magnetic resonance imaging as a surveillance tool for hepatocellular carcinoma: Comparison with ultrasound. J Hepatol. 72:718–724. 2020.PubMed/NCBI View Article : Google Scholar

43 

Kong S, Yue X, Kong S and Ren Y: Application of contrast-enhanced ultrasound and enhanced CT in diagnosis of liver cancer and evaluation of radiofrequency ablation. Oncol Lett. 16:2434–2438. 2018.PubMed/NCBI View Article : Google Scholar

44 

Galle PR, Foerster F, Kudo M, Chan SL, Llovet JM, Qin S, Schelman WR, Chintharlapalli S, Abada PB, Sherman M and Zhu AX: Biology and significance of alpha-fetoprotein in hepatocellular carcinoma. Liver Int. 39:2214–2229. 2019.PubMed/NCBI View Article : Google Scholar

45 

Yang Y, Yang M, Pang H, Qiu Y, Sun T, Wang T, Shen S and Wang W: A macrophage differentiation-mediated gene: DDX20 as a molecular biomarker encompassing the tumor microenvironment, disease staging, and prognoses in hepatocellular carcinoma. Oxid Med Cell Longev. 2022(9971776)2022.PubMed/NCBI View Article : Google Scholar

46 

Krajnović M, Kožik B, Božović A and Jovanović-Ćupić S: Multiple roles of the RUNX gene family in hepatocellular carcinoma and their potential clinical implications. Cells. 12(2303)2023.PubMed/NCBI View Article : Google Scholar

47 

Wang T, Jin H, Hu J, Li X, Ruan H, Xu H, Wei L, Dong W, Teng F, Gu J, et al: COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling. J Exp Clin Cancer Res. 39(148)2020.PubMed/NCBI View Article : Google Scholar

48 

Lin TC: RUNX1 and cancer. Biochim Biophys Acta Rev Cancer. 1877(188715)2022.PubMed/NCBI View Article : Google Scholar

49 

Liu Y, Wu Z, Zhao Y, Zhen M, Wang Y and Liu Q: Apolipoprotein H-based prognostic risk correlates with liver lipid metabolism disorder in patients with HBV-related hepatocellular carcinoma. Heliyon. 10(e31412)2024.PubMed/NCBI View Article : Google Scholar

50 

Huang C and Freter C: Lipid metabolism, apoptosis and cancer therapy. Int J Mol Sci. 16:924–949. 2015.PubMed/NCBI View Article : Google Scholar

51 

Lv S, Wang W, Wang H, Zhu Y and Lei C: PPARγ activation serves as therapeutic strategy against bladder cancer via inhibiting PI3K-Akt signaling pathway. BMC Cancer. 19(204)2019.PubMed/NCBI View Article : Google Scholar

52 

Cai HB, Zhao MY, Li XH, Li YQ, Yu TH, Wang CZ, Wang LN, Xu WY, Liang B, Cai YP, et al: Single cell sequencing revealed the mechanism of CRYAB in glioma and its diagnostic and prognostic value. Front Immunol. 14(1336187)2024.PubMed/NCBI View Article : Google Scholar

53 

Dai A, Guo X, Yang X, Li M, Fu Y and Sun Q: Effects of the CRYAB gene on stem cell-like properties of colorectal cancer and its mechanism. J Cancer Res Ther. 18:1328–1337. 2022.PubMed/NCBI View Article : Google Scholar

54 

Deng J, Chen X, Zhan T, Chen M, Yan X and Huang X: CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer. PeerJ. 9(e12578)2021.PubMed/NCBI View Article : Google Scholar

55 

Ruan H, Li Y, Wang X, Sun B, Fang W, Jiang S and Liang C: CRYAB inhibits migration and invasion of bladder cancer cells through the PI3K/AKT and ERK pathways. Jpn J Clin Oncol. 50:254–260. 2020.PubMed/NCBI View Article : Google Scholar

56 

Su CH, Liu LC, Hsieh YH, Wang HC, Tsai CW, Chang WS, Ho CY, Wu CI, Lin CH, Lane HY and Bau DT: Association of Alpha B-Crystallin (CRYAB) genotypes with breast cancer susceptibility in Taiwan. Cancer Genomics Proteomics. 8:251–254. 2011.PubMed/NCBI

57 

Lu R, Tang P, Zhang D, Lin S, Li H, Feng X, Sun M and Zhang H: SOX9/NFIA promotes human ovarian cancer metastasis through the Wnt/β-catenin signaling pathway. Pathol Res Pract. 248(154602)2023.PubMed/NCBI View Article : Google Scholar

58 

Hu Y, Zhang Y, Ding M and Xu R: Lncrna linc00511 acts as an oncogene in colorectal cancer via sponging mir-29c-3p to upregulate nfia. Onco Targets Ther. 13:13413–13424. 2021.PubMed/NCBI View Article : Google Scholar

59 

Sun C, Li Y, Tan Y, Zhang H, Liang Y, Zeng J, Yu J and Zou H: A novel role for NFIA in restoring radiosensitivity in radioresistant NSCLC cells by downregulating the AKT and ERK pathways. Biochem Biophys Res Commun. 515:558–564. 2019.PubMed/NCBI View Article : Google Scholar

60 

Zhu Z, Luo L, Xiang Q, Wang J, Liu Y, Deng Y and Zhao Z: MiRNA-671-5p Promotes prostate cancer development and metastasis by targeting NFIA/CRYAB axis. Cell Death Dis. 11(949)2020.PubMed/NCBI View Article : Google Scholar

61 

Shi C, Yang X, Bu X, Hou N and Chen P: Alpha B-crystallin promotes the invasion and metastasis of colorectal cancer via epithelial-mesenchymal transition. Biochem Biophys Res Commun. 489:369–374. 2017.PubMed/NCBI View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jin Y, Hu P, Dai Y, Gu W, Han J and Song H: Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression. Mol Clin Oncol 23: 72, 2025.
APA
Jin, Y., Hu, P., Dai, Y., Gu, W., Han, J., & Song, H. (2025). Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression. Molecular and Clinical Oncology, 23, 72. https://doi.org/10.3892/mco.2025.2867
MLA
Jin, Y., Hu, P., Dai, Y., Gu, W., Han, J., Song, H."Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression". Molecular and Clinical Oncology 23.2 (2025): 72.
Chicago
Jin, Y., Hu, P., Dai, Y., Gu, W., Han, J., Song, H."Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression". Molecular and Clinical Oncology 23, no. 2 (2025): 72. https://doi.org/10.3892/mco.2025.2867
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Y, Hu P, Dai Y, Gu W, Han J and Song H: Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression. Mol Clin Oncol 23: 72, 2025.
APA
Jin, Y., Hu, P., Dai, Y., Gu, W., Han, J., & Song, H. (2025). Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression. Molecular and Clinical Oncology, 23, 72. https://doi.org/10.3892/mco.2025.2867
MLA
Jin, Y., Hu, P., Dai, Y., Gu, W., Han, J., Song, H."Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression". Molecular and Clinical Oncology 23.2 (2025): 72.
Chicago
Jin, Y., Hu, P., Dai, Y., Gu, W., Han, J., Song, H."Nuclear factor IA‑mediated transcriptional regulation of crystallin &alpha;B inhibits hepatocellular carcinoma progression". Molecular and Clinical Oncology 23, no. 2 (2025): 72. https://doi.org/10.3892/mco.2025.2867
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team