Integrated multi‑omics profiling of plasma extracellular vesicles reveals the hsa‑miR‑1‑3p‑LRP1 axis as a potential biomarker in cervical cancer

Bao,Tonghui; Li,Xiaoping; Li,Wufen; Yang,Rui; Ye,Youhong; Zhang,Wenjia; Qi,Lin

doi:10.3892/mco.2025.2922

February-2026 Volume 24 Issue 2

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February-2026 Volume 24 Issue 2

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Integrated multi‑omics profiling of plasma extracellular vesicles reveals the hsa‑miR‑1‑3p‑LRP1 axis as a potential biomarker in cervical cancer

Authors:
- Tonghui Bao
- Xiaoping Li
- Wufen Li
- Rui Yang
- Youhong Ye
- Wenjia Zhang
- Lin Qi
View Affiliations / Copyright

Affiliations: Department of Gynecology, Affiliated Hospital of Qinghai University, Xining, Qinghai 810000, P.R. China, Department of Geriatrics, Affiliated Hospital of Qinghai University, Xining, Qinghai 810000, P.R. China, Department of Hematology and Oncology, Qinghai Provincial Traditional Chinese Medicine Hospital, Xining, Qinghai 810000, P.R. China, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710000, P.R. China, Department of Gynecology, Affiliated Hospital of Qinghai University, Xining, Qinghai 810000, P.R. China, Department of Oncology and Gynecology, Affiliated Hospital of Qinghai University, Xining, Qinghai 810000, P.R. China

Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 13
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Published online on: December 31, 2025

https://doi.org/10.3892/mco.2025.2922
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Abstract

Cervical cancer (CC) is a malignancy characterized by persistent human papillomavirus (HPV) infection, immune evasion and tumor microenvironment remodeling. Due to its high invasiveness and drug resistance, effective diagnosis and treatment remain challenging. The present study employed multi‑omics analysis to explore the molecular characteristics of plasma extracellular vesicles (EVs) in CC. EVs were isolated from the plasma of 3 patients with CC and 3 healthy controls via ultracentrifugation, and validated by nanoparticle tracking analysis, transmission electron microscopy and western blotting. MicroRNA (miRNA or miR) sequencing identified 22 differentially expressed miRNAs, the target genes of which were enriched in HPV infection, p53, PI3K‑Akt and Wnt signaling pathways. Proteomic analysis revealed 49 differentially expressed proteins associated with complement activation, cholesterol metabolism and coagulation. Integrated analysis highlighted key miRNA‑protein interaction networks, identifying hsa‑miR‑1‑3p and LRP1 as potential diagnostic biomarkers, and their differential expression was further confirmed by reverse transcription‑quantitative PCR. These findings provide novel insights into the role of EVs in CC pathogenesis and offer promising potential targets for early diagnosis and therapeutic intervention.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Integrated multi‑omics profiling of plasma extracellular vesicles reveals the hsa‑miR‑1‑3p‑LRP1 axis as a potential biomarker in cervical cancer

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