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Article Open Access

Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells

  • Authors:
    • Mitsuru Chiba
    • Yuhei Ohsugi
    • Kana Matsumoto
    • Chisa Tayama
  • View Affiliations / Copyright

    Affiliations: Department of Bioscience and Laboratory Medicine, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan, Department of Medical Technology, School of Health Sciences, Hirosaki University, Hirosaki, Aomori 036‑8564, Japan
    Copyright: © Chiba et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].
  • Article Number: 7
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    Published online on: January 5, 2024
       https://doi.org/10.3892/mi.2024.131
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Abstract

Fatty liver is a condition of excessive triglyceride accumulation in hepatocytes. Additionally, hepatocytes exhibit a high degree of fat droplet accumulation during excessive alcohol consumption and metabolic syndrome. However, the molecular mechanisms involved in fat droplet formation remain unknown. The present study used an in vitro fatty liver formation model of the human liver cancer cell line, HepG2, to comprehensively search for fat droplet formation‑related genes, and which exhibit changes in expression during fat droplet formation. Microarray analysis with extracted total RNA determined the genes that are involved in fat droplet formation and their expression was confirmed using quantitative polymerase chain reaction following the culture of the HepG2 cells in culture medium containing 0, 50, 200 and 500 µM of oleic acid for 24 h. The results revealed 142 genes demonstrating increased expression levels by >2.0‑fold with oleic acid treatment and 426 genes demonstrating decreased expression levels. Perilipin 2 (PLIN2) was estimated as the gene most closely associated with fatty liver. Lipid droplet formation in the HepG2 cells induced by oleic acid led to the upregulation of PLIN2 in a concentration‑dependent manner. On the whole, the findings of the present study indicate the involvement of genes in oleic acid‑induced lipid droplet formation in HepG2 cells; PLIN2 in particular may play a crucial role in this process.
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1 

Zhu B, Chan SL, Li J, Li K, Wu H, Cui K and Chen H: Non-alcoholic steatohepatitis pathogenesis, diagnosis, and treatment. Front Cardiovasc Med. 8(742382)2021.PubMed/NCBI View Article : Google Scholar

2 

Dufour JF, Anstee QM, Bugianesi E, Harrison S, Loomba R, Paradis V, Tilg H, Wong VW and Zelber-Sagi S: Current therapies and new developments in NASH. Gut. 71:2123–2134. 2022.PubMed/NCBI View Article : Google Scholar

3 

Shah PA, Patil R and Harrison SA: NAFLD-related hepatocellular carcinoma: The growing challenge. Hepatology. 77:323–338. 2023.PubMed/NCBI View Article : Google Scholar

4 

Santos JPMD, Maio MC, Lemes MA, Laurindo LF, Haber JFDS, Bechara MD, Prado PSD Jr, Rauen EC, Costa F, Pereira BCA, et al: Non-alcoholic steatohepatitis (NASH) and organokines: What is now and what will be in the future. Int J Mol Sci. 23(498)2022.PubMed/NCBI View Article : Google Scholar

5 

Zeigerer A, Rodeheffer MS, McGraw TE and Friedman JM: Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism. Exp Cell Res. 314:2249–2256. 2008.PubMed/NCBI View Article : Google Scholar

6 

Tie F, Ding J, Hu N, Dong Q, Chen Z and Wang H: Kaempferol and kaempferide attenuate oleic acid-induced lipid accumulation and oxidative stress in HepG2 cells. Int J Mol Sci. 22(8847)2021.PubMed/NCBI View Article : Google Scholar

7 

Scorletti E and Carr RM: A new perspective on NAFLD: Focusing on lipid droplets. J Hepatol. 76:934–945. 2022.PubMed/NCBI View Article : Google Scholar

8 

Nettebrock NT and Bohnert M: Born this way-Biogenesis of lipid droplets from specialized ER subdomains. Biochim Biophys Acta Mol Cell Biol Lipids. 1865(158448)2020.PubMed/NCBI View Article : Google Scholar

9 

Chiba M, Kubota S, Sato K and Monzen S: Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro. Sci Rep. 8(11972)2018.PubMed/NCBI View Article : Google Scholar

10 

Chiba M, Kimura M and Asari S: Exosomes secreted from human colorectal cancer cell lines contain mRNAs, microRNAs and natural antisense RNAs, that can transfer into the human hepatoma HepG2 and lung cancer A549 cell lines. Oncol Rep. 28:1551–1558. 2012.PubMed/NCBI View Article : Google Scholar

11 

Chiba M: Differential expression of natural antisense transcripts during liver development in embryonic mice. Biomed Rep. 2:918–922. 2014.PubMed/NCBI View Article : Google Scholar

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001.PubMed/NCBI View Article : Google Scholar

13 

Alonso-Peña M, Del Barrio M, Peleteiro-Vigil A, Jimenez-Gonzalez C, Santos-Laso A, Arias-Loste MT, Iruzubieta P and Crespo J: Innovative therapeutic approaches in non-alcoholic fatty liver disease: When knowing your patient is key. Int J Mol Sci. 24(10718)2023.PubMed/NCBI View Article : Google Scholar

14 

Gómez-Lechón MJ, Donato MT, Martínez-Romero A, Jiménez N, Castell JV and O'Connor JE: A human hepatocellular in vitro model to investigate steatosis. Chem Biol Interact. 165:106–116. 2007.PubMed/NCBI View Article : Google Scholar

15 

Campos-Espinosa A and Guzmán C: A model of experimental steatosis in vitro: Hepatocyte cell culture in lipid overload-conditioned medium. J Vis Exp. 171(e62543)2021.PubMed/NCBI View Article : Google Scholar

16 

Okumura T: Role of lipid droplet proteins in liver steatosis. J Physiol Biochem. 67:629–636. 2011.PubMed/NCBI View Article : Google Scholar

17 

Pereira-Dutra FS and Bozza PT: Lipid droplets diversity and functions in inflammation and immune response. Expert Rev Proteomics. 18:809–825. 2021.PubMed/NCBI View Article : Google Scholar

18 

Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D, Odeberg J, Habuka M, Tahmasebpoor S, Danielsson A, Edlund K, et al: Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics. 13:397–406. 2014.PubMed/NCBI View Article : Google Scholar

19 

Jin Y, Tan Y, Chen L, Liu Y and Ren Z: Reactive oxygen species induces lipid droplet accumulation in HepG2 cells by increasing perilipin 2 expression. Int J Mol Sci. 19(3445)2018.PubMed/NCBI View Article : Google Scholar

20 

Nocetti D, Espinosa A, Pino-De la Fuente F, Sacristán C, Bucarey JL, Ruiz P, Valenzuela R, Chouinard-Watkins R, Pepper I, Troncoso R and Puente L: Lipid droplets are both highly oxidized and Plin2-covered in hepatocytes of diet-induced obese mice. Appl Physiol Nutr Metab. 45:1368–1376. 2020.PubMed/NCBI View Article : Google Scholar

21 

Griffin JD, Bejarano E, Wang XD and Greenberg AS: Integrated action of autophagy and adipose tissue triglyceride lipase ameliorates diet-induced hepatic steatosis in liver-specific PLIN2 knockout mice. Cells. 10(1016)2021.PubMed/NCBI View Article : Google Scholar

22 

Mak KM, Ren C, Ponomarenko A, Cao Q and Lieber CS: Adipose differentiation-related protein is a reliable lipid droplet marker in alcoholic fatty liver of rats. Alcohol Clin Exp Res. 32:683–689. 2008.PubMed/NCBI View Article : Google Scholar

23 

Carr RM, Peralta G, Yin X and Ahima RS: Absence of perilipin 2 prevents hepatic steatosis, glucose intolerance and ceramide accumulation in alcohol-fed mice. PLoS One. 9(e97118)2014.PubMed/NCBI View Article : Google Scholar

24 

Faulkner CS, White CM, Shah VH and Jophlin LL: A single nucleotide polymorphism of PLIN2 is associated with nonalcoholic steatohepatitis and causes phenotypic changes in hepatocyte lipid droplets: A pilot study. Biochim Biophys Acta Mol Cell Biol Lipids. 1865(158637)2020.PubMed/NCBI View Article : Google Scholar

25 

Teixeira FS, Pimentel LL, Pintado ME and Rodríguez-Alcalá LM: Impaired hepatic lipid metabolism and biomarkers in fatty liver disease. Biochimie. 215:69–74. 2023.PubMed/NCBI View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Chiba M, Ohsugi Y, Matsumoto K and Tayama C: Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells. Med Int 4: 7, 2024.
APA
Chiba, M., Ohsugi, Y., Matsumoto, K., & Tayama, C. (2024). Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells. Medicine International, 4, 7. https://doi.org/10.3892/mi.2024.131
MLA
Chiba, M., Ohsugi, Y., Matsumoto, K., Tayama, C."Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells". Medicine International 4.1 (2024): 7.
Chicago
Chiba, M., Ohsugi, Y., Matsumoto, K., Tayama, C."Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells". Medicine International 4, no. 1 (2024): 7. https://doi.org/10.3892/mi.2024.131
Copy and paste a formatted citation
x
Spandidos Publications style
Chiba M, Ohsugi Y, Matsumoto K and Tayama C: Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells. Med Int 4: 7, 2024.
APA
Chiba, M., Ohsugi, Y., Matsumoto, K., & Tayama, C. (2024). Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells. Medicine International, 4, 7. https://doi.org/10.3892/mi.2024.131
MLA
Chiba, M., Ohsugi, Y., Matsumoto, K., Tayama, C."Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells". Medicine International 4.1 (2024): 7.
Chicago
Chiba, M., Ohsugi, Y., Matsumoto, K., Tayama, C."Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells". Medicine International 4, no. 1 (2024): 7. https://doi.org/10.3892/mi.2024.131
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